基于UHPLC-QE-Orbitrap-MS技术的深加工槟榔籽的成分分析

doi:10.11843/j.issn.0366-6964.2023.12.036

摘要/Abstract

摘要： 本研究以槟榔深加工过程中产生的副产物槟榔籽为研究对象,采用超高效液相色谱-四极杆-静电场轨道肼高分辨质谱法(UHPLC-QE-Orbitrap-MS)对槟榔籽的成分进行分析鉴定。采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8 μm),以0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B)为流动相进行梯度洗脱,流速0.3 mL·min^-1,柱温35 ℃,进样体积10 μL,使用电喷雾离子源(ESI),扫描范围m/z为100~1 200,Full MS-ddMS²检测模式,正负离子同时采集。利用Compound Discoverer 3.3软件对原始数据进行分析,通过本地数据库和mzCloud在线数据库对化合物进行结构鉴定。试验鉴定出85种存在于槟榔籽中的成分,分别为生物碱类化合物3种、黄酮类化合物24种、有机酸类化合物15种、酚类化合物7种、萜类化合物7种、氨基酸类化合物6种、糖类化合物4种、酯类化合物4种、酰胺类化合物2种、鞘脂类化合物2种、类固醇衍生物1种、苯丙素类化合物1种、香豆素类化合物1种、其它8种。UHPLC-QE-Orbitrap-MS检测技术可以对槟榔深加工副产物槟榔籽中的成分进行快速识别,本研究为槟榔资源进一步综合利用,开发高附加值产品提供科学依据。

关键词: 槟榔籽, 副产物, 超高效液相色谱-四极杆-静电场轨道肼高分辨质谱法, 成分

Abstract: To study the chemical composition of Areca catechu L. seed in deep processing, UHPLC-QE-Orbitrap-MS technology was used to analyze and identify the composition of Areca catechu L. seed. Gradient elution was performed on ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm) with 0.1% formic acid aqueous solution (A) to 0.1% formic acid acetonitrile solution (B) as mobile phases. The flow rate was 0.3 mL·min^-1, the column temperature was 35 ℃, and the sample volume was 10 μL. An electric spray ion source (ESI) was used with a scanning range of 100-1 200 m/z and positive and negative ions were collected simultaneously, and the detection mode was Full MS-ddMS². The original data were analyzed using Compound Discoverer 3.3 software, and the structures of the compounds were identified using the local database and mzCloud online database. Eighty-five compounds of Areca catechu L. seed in deep processing were detected, including 3 alkaloids, 24 flavonoids, 15 organic acids, 7 phenols, 7 terpenoids, 6 amino acids, 4 carbohydrates, 4 esters, 2 amides, 2 sphingolipids, 1 steroid, 1 phenylpropanoid, 1 coumarin and 8 other species. The UHPLC-QE-Orbitrap-MS detection technology quickly identified the compound of Areca catechu L. seed in deep processing, and the research results provided scientific basis for exploring the application prospects and developing high value-added products of Areca catechu L.

Key words: the seed of Areca catechu L., by-product, UHPLC-QE-Orbitrap-MS, composition

中图分类号:

S853.7

马丹阳, 高雪嫣, 彭凌峰, 伍少峰, 王庆涛, 郝智慧. 基于UHPLC-QE-Orbitrap-MS技术的深加工槟榔籽的成分分析[J]. 畜牧兽医学报, 2023, 54(12): 5275-5292.

MA Danyang, GAO Xueyan, PENG Lingfeng, WU Shaofeng, WANG Qingtao, HAO Zhihui. Composition Analysis of Seed of Areca catechu L. in Deep Processing based on UHPLC-QE-Orbitrap-MS Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5275-5292.

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