HMGB1与日本血吸虫病肝炎症和纤维化的相关性分析

doi:10.11843/j.issn.0366-6964.2022.10.032

摘要/Abstract

摘要： 旨在探究高迁移率族蛋白B1（high mobility group box 1，HMGB1）与日本血吸虫病肝炎症和纤维化的相关性，本研究将BALB/c小鼠随机分为空白对照组（n＝10），感染后4周组、6周组和HMGB1抑制剂组（n＝5），进行血常规及生化检测，观察肝病理学及Masson染色情况、荧光定量PCR检测HMGB1、IL-1β、TNF-α、IL-6、IFN-γ、α-SMA、Col1α1、TGF-β1、Smad2和Smad3的转录水平。结果显示：感染后4周，小鼠谷丙转氨酶（alanine aminotransferase，ALT）、肝组织HMGB1、α-SMA、TGF-β1、IL-6和 TNF-α的转录水平均显著或极显著升高（P<0.05或P < 0.01）；感染后6周，小鼠WBC、Neu、Mon、Eos、谷草转氨酶（aspartate aminotransferase，AST）和ALT极显著升高（P<0.01），肝组织HMGB1、IL-1β、TNF-α、IL-6、IFN-γ、α-SMA、Col1α1、TGF-β1、Smad2和Smad3的转录水平均显著或极显著升高（P<0.05或P<0.01）；感染后6周小鼠肝虫卵聚积处伴有炎性细胞浸润和胶原纤维增生；HMGB1抑制剂可显著降低小鼠血清ALT值和肝组织HMGB1、α-SMA、TGF-β1、IL-6和TNF-α的转录水平（P<0.05）。综上表明，HMGB1与日本血吸虫病肝炎症和纤维化具有一定相关性。

关键词: 日本血吸虫, 肝纤维化, 炎症, 高迁移率族蛋白B1

Abstract: To investigate the correlation of high mobility group box 1 (HMGB1) with liver inflammatory fibrosis induced by Schistosoma japonicum infection, in the present study, BALB/c mice were randomly divided into control group (n=10), 4 weeks' infection group, 6 weeks' infection group and HMGB1 inhibited group (n=5). Blood routine tests and biochemical tests were performed as well as liver pathology observation and Masson staining. qRT-PCR was used to detect the transcription levels of HMGB1,IL-1β,TNF-α,IL-6,IFN-γ,α-SMA,Col1α1,TGF-β1,Smad2 and Smad3 in the liver. Results showed that 4 weeks after infection, serum alanine aminotransferase (ALT) and the transcription levels of HMGB1, α-SMA, TGF-β1, IL-6 and TNF-α were increased significantly (P<0.05 or P<0.01); 6 weeks after infection, WBC, Neu, Mon, Eos, aspartate aminotransferase and ALT were significantly increased (P<0.01), the transcription levels of HMGB1, IL-1β, TNF-α, IL-6, IFN-γ, α-SMA, Col1α1, TGF-β1, Smad2 and Smad3 were significantly increased as well (P<0.05 or P<0.01); 6 weeks after infection, large number of eggs were deposited in the liver accompanied with neutrophils infiltration and collagen fiber accumulation. The HMGB1 inhibitor could significantly reduce the ALT level and transcription levels of HMGB1, α-SMA, TGF-β1, IL-6 and TNF-α in the liver (P<0.05). In conclusion, there was a correlation between HMGB1 and liver inflammatory fibrosis induced by S. japonicum infection.

Key words: Schistosoma japonicum, liver fibrosis, inflammation, high mobility group box 1

中图分类号:

钟昊然, 侯玲, 桂祥, 吕荣雪, 刘金明, 古少鹏, 金亚美. HMGB1与日本血吸虫病肝炎症和纤维化的相关性分析[J]. 畜牧兽医学报, 2022, 53(10): 3609-3620.

ZHONG Haoran, HOU Ling, GUI Xiang, LÜ Rongxue, LIU Jinming, GU Shaopeng, JIN Yamei. The Correlation of HMGB1 with Liver Inflammatory Fibrosis Induced by Schistosoma japonicum[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3609-3620.

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