新型生物组织工程角膜移植治疗犬猫角膜疾病的回顾性分析

doi:10.11843/j.issn.0366-6964.2023.10.035

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (10): 4389-4402.doi: 10.11843/j.issn.0366-6964.2023.10.035

新型生物组织工程角膜移植治疗犬猫角膜疾病的回顾性分析

胥辉豪^1,2*, 焦弋恩¹, 郑小波¹, 张志鹏³, 杨丽辉⁴, 林金洲⁵, 王力波⁶, 唐静¹, 李启卷¹, 刘江渝¹, 陈子洋¹, 胡小艳¹, 朴雪玲¹, 张慧¹, 甘玲^1*

1. 西南大学动物医学院, 重庆 402460;
2. 西南大学发光分析与分子传感教育部重点实验室, 重庆 400700;
3. 杭州瑞派虹泰宠物医院, 杭州 310004;
4. 维特(深圳)动物医院, 深圳 518000;
5. 厦门御宝贝宠物医院, 厦门 361001;
6. 宠颐生沈阳中心医院, 沈阳 110023

收稿日期:2023-02-17 出版日期:2023-10-23 发布日期:2023-10-26
通讯作者: 胥辉豪,E-mail:xuhuihao2dai@163.com;甘玲,主要从事动物应激损伤与营养调节研究,E-mail:260994348@qq.com
作者简介:胥辉豪(1983-),男,重庆人,高级实验师,博士,主要从事兽医外、产科学、兽医眼科学与肿瘤学研究,Tel:023-68251603
基金资助:
西南大学实验技术研究项目（SYJ2020013）；深圳市瑞鹏公益基金会高校青年教师（小动物临床）科研基金（RPJJ2020001）

A Large Retrospective Study on the Treatment of Corneal Diseases in Dogs and Cats with a New Type of Bioengineered Cornea Transplantation

XU Huihao^1,2*, JIAO Yien¹, ZHENG Xiaobo¹, ZHANG Zhipeng³, YANG Lihui⁴, LIN Jinzhou⁵, WANG Libo⁶, TANG Jing¹, LI Qijuan¹, LIU Jiangyu¹, CHEN Ziyang¹, HU Xiaoyan¹, PIAO Xueling¹, ZHANG Hui¹, GAN Ling^1*

1. College of Veterinary Medicine, Southwest University, Chongqing 402460, China;
2. Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing 400700, China;
3. Hong Tai Pet Hospital, Hangzhou 310004, China;
4. IVC Shenzhen Animal Hospital, Shenzhen 518000, China;
5. PRO Pet Hospital, Xiamen 361001, China;
6. Chongyisheng Shenyang Central Hospital, Shenyang 110023, China

Received:2023-02-17 Online:2023-10-23 Published:2023-10-26

摘要/Abstract

摘要： 本研究旨在大型回顾性分析总结新型生物组织工程角膜（biotissue-engineered cornea，BEC）治疗犬猫角膜疾病的手术方法与长期临床效果。本研究回顾了2018—2022年使用BEC进行眼表移植手术并至少跟踪随访3个月及以上的犬猫的医疗记录。包括坏死性角膜炎、深度角膜溃疡、后弹力层膨出以及角膜穿孔在内的犬猫患眼被纳入本研究。术后每周定期检查，并在术后0、3、6和12个月对部分患眼进行了光学相干断层扫描检查，根据最后一次检查结果对术眼角膜进行评级。本研究共纳入139只患眼（猫112例，犬27例），其中板层角膜移植103只眼，全层角膜移植36只眼。使用4种不同厚度的BEC植片（200、300、400和450 μm），植片的平均直径为7.74 mm （范围为3~13 mm）。术后影响相对较大的并发症包括植片部分松脱或裂开（5例，3.60%）、植片溶解或穿孔（8例，5.76%）、角膜植床坏死（1例，0.72%）和眼球萎缩（1例，0.72%）。术后随访时间为3~50个月（中位数18个月），最终139只眼均保持了角膜和眼球完整性，其中133只眼（95.68%）保留了较敏锐视力。统计学分析显示，角膜植片透明度与动物年龄（P=0.021）、物种（P=0.000）、植片直径（P=0.032）、是否全层移植（P=0.000）以及角膜再上皮时间（P=0.000）显著正相关。BEC是一种安全且有效的角膜植片，可用于犬猫板层或全层角膜移植术，具有广泛的临床应用价值。

关键词: 犬, 猫, 角膜移植, 生物组织工程角膜

Abstract: This study was conducted to retrospectively analyze and summarize the surgical methods and long-term clinical effects of the new biotissue-engineered cornea (BEC) in the treatment of corneal diseases in canine and feline. This study reviewed the medical records of canine and feline useing BEC for ocular surface transplantation from 2018 to 2022 and followed up for at least 3 months. The eyes of animals including sequestrum, deep corneal ulcer, descemetocele and corneal perforation were included in the study. The patients were examined regularly every week after surgery, and selected eyes were examined with optical coherence tomography at 0, 3, 6 and 12 months after surgery. The corneas of the patients were graded according to the results of the last examination. A total of 139 eyes (112 of feline and 27 of canine) were included in this study, including 103 eyes with lamellar keratoplasty and 36 eyes with full-thickness keratoplasty. Four different thicknesses of BEC implants (200, 300, 400 and 450 μm) were used. The average diameter of the graft was 7.74 mm (range:3-13 mm). Postoperative complications with relatively impact included partial graft loosening or dehiscence (5 cases, 3.60%), graft dissolution or perforation (8 cases, 5.76%), corneal graft bed sequestrum (1 case, 0.72%) and atrophia bulbi (1 case, 0.72%). The follow-up time spanned 3-50 months (median 18 months). Finally, 139 eyes maintained the integrity of cornea and eyeball, and 133 eyes (95.68%) retained relatively sharp vision. Statistical analysis showed that corneal graft transparency was significantly and positively correlated with animal age (P=0.021), species (P=0.000), Graft diameter (P=0.032), full thickness transplantation or not (P=0.000), and corneal re-epithelialization time (P=0.000). BEC is a safe and effective corneal graft, which can be used for lamellar or full-thickness keratoplasty in canine and feline, and has extensive clinical application value.

Key words: canine, feline, corneal transplant, biotissue-engineered cornea

中图分类号:

S857.6

胥辉豪, 焦弋恩, 郑小波, 张志鹏, 杨丽辉, 林金洲, 王力波, 唐静, 李启卷, 刘江渝, 陈子洋, 胡小艳, 朴雪玲, 张慧, 甘玲. 新型生物组织工程角膜移植治疗犬猫角膜疾病的回顾性分析[J]. 畜牧兽医学报, 2023, 54(10): 4389-4402.

XU Huihao, JIAO Yien, ZHENG Xiaobo, ZHANG Zhipeng, YANG Lihui, LIN Jinzhou, WANG Libo, TANG Jing, LI Qijuan, LIU Jiangyu, CHEN Ziyang, HU Xiaoyan, PIAO Xueling, ZHANG Hui, GAN Ling. A Large Retrospective Study on the Treatment of Corneal Diseases in Dogs and Cats with a New Type of Bioengineered Cornea Transplantation[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4389-4402.

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新型生物组织工程角膜移植治疗犬猫角膜疾病的回顾性分析

A Large Retrospective Study on the Treatment of Corneal Diseases in Dogs and Cats with a New Type of Bioengineered Cornea Transplantation

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