犬子宫蓄脓组织的转录组学分析

doi:10.11843/j.issn.0366-6964.2023.08.023

摘要/Abstract

摘要： 子宫蓄脓是一种在子宫腔内积聚脓液的常见的犬类疾病，是全世界犬类死亡的主要原因。然而，子宫蓄脓的潜在机制仍知之甚少。本研究旨在探究犬子宫蓄脓的转录组水平变化，筛选差异表达基因、调控通路以及中枢基因，以进一步了解子宫蓄脓的分子机制。本研究通过RNA-Seq比较子宫蓄脓犬与健康犬转录组水平变化。结果显示共有11 812个基因表达存在差异，其中7 086个基因在患犬的子宫中上调，而4 726个基因下调。GO功能分析显示，差异表达基因主要涉及免疫系统过程、细胞因子活性、趋化因子活性、细胞因子受体结合等生物过程。KEGG-Pathway分析发现，差异表达基因参与炎症与免疫反应激活的信号通路，例如NF-κB信号通路、PI3K-Akt信号通路、B细胞受体信号通路、吞噬体和自然杀伤细胞介导的细胞毒性，说明炎症和免疫反应激活是犬子宫蓄脓的主要病理阶段。通过使用STRING数据库网络，确定了11个关键中枢基因（如CDK1、ESR1和SHC1等），其产物可能作为诊断子宫蓄脓的分子标记出现。综上，本研究展示了犬子宫蓄脓的基因表达的综合概况，有助于更好地了解子宫蓄脓的分子机制。

关键词: 犬, 子宫蓄脓, RNA-seq, 炎症, 免疫系统

Abstract: Pyometra, a common canine disease accompanied by bacterial contamination in the uterus, is a major cause of canine death worldwide. However, the underlying mechanism of pyometra remains poorly understood. The purpose of this study is to identify the gene expression changes associated with canine pyometra, and to screen differentially expressed genes, regulatory pathways and hub genes to further understand the molecular mechanism of pyometra. RNA sequencing (RNA-seq) was uesd to study the gene expression profile of canines with pyometra compared with those with benign uterus. The results showed a discrepancy of 11 812 gene expressions, among which 7 086 genes were up-regulated while 4 726 genes were down-regulated in the uteri of diseased animals. GO analysis showed that differentially expressed genes were mainly involved in biological processes such as immune system processes, cytokine activity, chemokine activity, and cytokine receptor binding. KEGG-Pathway analysis found that differentially expressed genes were involved in the signaling pathways of inflammation and immune response activation, such as NF-κB signaling pathway, PI3K-Akt signaling pathway, B cell receptor signaling pathway, phagosome and natural killer cell-mediated cytotoxicity, revealing that inflammation and immune response activation are the main pathological stages of canine pyometra. Eleven key hub genes were identified by STRING database network, such as CDK1, ESR1 and SHC1, which can be used as biomarkers for early diagnosis of pyometra. In conclusion, the current study presents a comprehensive landscape of the gene expression in pyometra canines and contribute to a better understanding of the molecular mechanisms of canine pyometra.

Key words: canine, pyometra, RNA-seq, inflammation, immune system

中图分类号:

S858.292.724

钟华, 宋杉杉, 邵浣婷, 赵瑜, 康劲文, 吴瑶, 苏仁伟. 犬子宫蓄脓组织的转录组学分析[J]. 畜牧兽医学报, 2023, 54(8): 3383-3392.

ZHONG Hua, SONG Shanshan, SHAO Huanting, ZHAO Yu, KANG Jinwen, WU Yao, SU Renwei. Transcriptome Sequencing Analysis on Canine Pyometra Uterine Tissue[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3383-3392.

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