牛病毒性腹泻病毒蛋白的免疫学特性以及相关疫苗研究进展

doi:10.11843/j.issn.0366-6964.2023.04.004

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (4): 1381-1391.doi: 10.11843/j.issn.0366-6964.2023.04.004

牛病毒性腹泻病毒蛋白的免疫学特性以及相关疫苗研究进展

李易聪^1,2,3, 蒲飞洋^1,2,3, 冯茜莉^1,2,3, 汪梦竹^1,2,3, 赵泽阳^1,2,3, 张德荣^1,2, 马忠仁^1,2, 周建华^1,2,4*

1. 西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室, 兰州 730030;
2. 西北民族大学生物医学研究中心甘肃省动物细胞技术创新中心, 兰州 730030;
3. 西北民族大学生命科学与工程学院, 兰州 730010;
4. 中国农业科学院兰州兽医研究所, 兰州 730046

收稿日期:2022-04-26 出版日期:2023-04-23 发布日期:2023-04-27
通讯作者: 周建华,主要从事病毒基因工程研究,E-mail:zhoujianhuazjh@163.com
作者简介:李易聪(1998-),女,山西霍州人,硕士生,主要从事病毒基因工程研究,E-mail:lyicongcong@163.com
基金资助:
优秀青年团队培育项目（31920220069）；西北民族大学引进人才科研项目（xbmuyjrc202225）；国家自然科学基金（31302100）

Research Progress on Immunological Characteristics of Bovine Viral Diarrhea Virus Protein and Vaccines

LI Yicong^1,2,3, PU Feiyang^1,2,3, FENG Xili^1,2,3, WANG Mengzhu^1,2,3, ZHAO Zeyang^1,2,3, ZHANG Derong^1,2, MA Zhongren^1,2, ZHOU Jianhua^1,2,4*

1. Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China;
2. Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China;
3. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730010, China;
4. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:2022-04-26 Online:2023-04-23 Published:2023-04-27

摘要/Abstract

摘要： 牛病毒性腹泻病毒（bovine viral diarrhea virus，BVDV）众多基因亚型毒株的流行及其宿主谱的扩大干扰着BVDV防控工作。欧洲国家采用全面检测和扑杀BVDV阳性牛的方法达到一定防控效果，但结合我国实际生产情况，使用疫苗防控BVDV仍是最优策略。深入了解病毒感染过程中BVDV蛋白与宿主免疫系统之间的“博弈”，将有助于研究人员在疫苗研发过程中有效规避抗原候选蛋白可能对免疫效果造成的干扰。此外，大力开发低成本、高效力的新型疫苗（如表位肽疫苗和病毒样颗粒疫苗）可以在整合病毒免疫优势抗原表位的基础上加强BVDV相关疫苗的生物安全性，满足用疫苗防控BVDV的要求。鉴于此，本文对BVDV蛋白免疫学功能与特性的研究以及BVDV疫苗开发的现状进行综述，以期为BVDV疫苗研发以及BVDV防控提供一些理论指导。

关键词: 牛病毒性腹泻病毒(BVDV), BVDV蛋白, 疫苗, 免疫

Abstract: The prevalence of numerous genetic subtypes strains of bovine viral diarrhea virus (BVDV) and the expansion of its host spectrum interfere with the prevention and control of BVDV. European countries have adopted the measure of comprehensive detection and culling of BVDV-positive cattle and achieved a certain prevention and control effect, but considering the actual production situation in China, vaccination is still the optimal strategy. An in-depth understanding of the ‘game’ between BVDV proteins and the host immune system during virus infection will help researchers to effectively avoid the potential interference of antigen candidate proteins on immune effects in the term of vaccine development. In addition, vigorously developing low-cost, high-efficiency new-type vaccines (such as epitope peptide vaccine and virus-like particle vaccine) can enhance the biosafety of BVDV vaccines based on integrating viral immunodominant antigenic epitopes, and meet the requirements for the prevention and control of BVDV with vaccines. Thus, in order to provide proof-of-concept towards the development of BVDV vaccine and BVDV prevention and control, this paper reviews the researches on the immunological function and characteristics of BVDV proteins and the current situation of BVDV vaccine development.

Key words: bovine viral diarrhea virus (BVDV), BVDV proteins, vaccines, immunity

中图分类号:

S852.653

李易聪, 蒲飞洋, 冯茜莉, 汪梦竹, 赵泽阳, 张德荣, 马忠仁, 周建华. 牛病毒性腹泻病毒蛋白的免疫学特性以及相关疫苗研究进展[J]. 畜牧兽医学报, 2023, 54(4): 1381-1391.

LI Yicong, PU Feiyang, FENG Xili, WANG Mengzhu, ZHAO Zeyang, ZHANG Derong, MA Zhongren, ZHOU Jianhua. Research Progress on Immunological Characteristics of Bovine Viral Diarrhea Virus Protein and Vaccines[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1381-1391.

参考文献 67

[1]	BENAVIDES B, CASAL J, DIÉGUEZ J F, et al.Development of a quantitative risk assessment of bovine viral diarrhea virus and bovine herpesvirus-1 introduction in dairy cattle herds to improve biosecurity[J].J Dairy Sci, 2020, 103(7):6454-6472.
[2]	YŞILBAǦ K, ALPAY G, BECHER P.Variability and global distribution of subgenotypes of bovine viral diarrhea virus[J].Viruses, 2017, 9(6):128.
[3]	BAUERMANN F V, RIDPATH J F.HoBi-like viruses-the typical ‘atypical bovine pestivirus’[J].Anim Health Res Rev, 2015, 16(1):64-69.
[4]	KING A M Q, LEFKOWITZ E J, MUSHEGIAN A R, et al.Changes to taxonomy and the international code of virus classification and nomenclature ratified by the international committee on taxonomy of viruses (2018)[J].Arch Virol, 2018, 163(9):2601-2631.
[5]	OǦUZOǦLU T Ç, KOÇ B T, COŞKUN N, et al.Endless variety for bovine virus diarrhea viruses:new members of a novel subgroup into Pestivirus A from Turkey[J].Trop Anim Health Prod, 2019, 51(5):1083-1087.
[6]	AL-KUBATI A A G, HUSSEN J, KANDEEL M, et al.Recent advances on the bovine viral diarrhea virus molecular pathogenesis, immune response, and vaccines development[J].Front Vet Sci, 2021, 8:665128.
[7]	ZHOU J H, GAO Z L, ZHANG J, et al.Comparative the codon usage between the three main viruses in pestivirus genus and their natural susceptible livestock[J].Virus Genes, 2012, 44(3):475-481.
[8]	杞艳萍.西部四省(区)BVDV的流行病学调查及分离鉴定[D].杨凌:西北农林科技大学, 2021.QI Y P.Epidemiological investigation and identification of BVDV in four western provinces (regions)[D].Yangling:Northwest A&F University, 2021.(in Chinese)
[9]	薛飞, 朱远茂, 马磊.我国牛病毒性腹泻/黏膜病研究进展及防控策略[J].中国奶牛, 2016(11):25-29.XUE F, ZHU Y M, MA L.Research progress and prevention and control strategies of bovine viral diarrhea/mucosal disease in China[J].China Dairy Cattle, 2016(11):25-29.(in Chinese)
[10]	MA P, MA X X, CHANG Q Y, et al.The effects of nucleotide usage in key nucleotide positions +4 and -3 flanking start codon on translation levels mediated by IRES of hepatitis C virus[J].Acta Virol, 2018, 62(4):441-446.
[11]	KOKKONOS K G, FOSSAT N, NIELSEN L, et al.Evolutionary selection of pestivirus variants with altered or no microRNA dependency[J].Nucleic Acids Res, 2020, 48(10):5555-5571.
[12]	SANGEWAR N, WAGHELA S D, YAO J, et al.Novel potent IFN-γ-inducing CD8+T cell epitopes conserved among diverse bovine viral diarrhea virus strains[J].J Immunol, 2021, 206(8):1709-1718.
[13]	常秋燕, 郭富城, 冶昡青, 等.牛病毒性腹泻病毒Npro蛋白的原核表达及裂解效率[J].江苏农业学报, 2019, 35(5):1161-1166.CHANG Q Y, GUO F C, YE X Q, et al.Prokaryotic expression and splitting decomposition rate of Npro protein of bovine viral diarrhea virus[J].Jiangsu Journal of Agricultural Sciences, 2019, 35(5):1161-1166.(in Chinese)
[14]	DARWEESH M F, RAJPUT M K S, BRAUN L J, et al.BVDV Npro protein mediates the BVDV induced immunosuppression through interaction with cellular S100A9 protein[J].Microb Pathog, 2018, 121:341-349.
[15]	陈鑫烨.牛病毒性腹泻病毒抗原表位的筛选及其单克隆抗体的制备[D].扬州:扬州大学, 2020.CHEN X Y.Screening of a BVDV epitope and preparation of monoclonal antibodies[D].Yangzhou:Yangzhou University, 2020.(in Chinese)
[16]	GONG X W, CHEN Q W, ZHENG F Y.Identification of protein inhibitor of activated STAT 4, a novel host interacting partner that involved in bovine viral diarrhea virus growth[J].Virol J, 2020, 17(1):59.
[17]	ELAHI S M, SHEN S H, TALBOT B G, et al.Induction of humoral and cellular immune responses against the nucleocapsid of bovine viral diarrhea virus by an adenovirus vector with an inducible promoter[J].Virology, 1999, 261(1):1-7.
[18]	CHEN X Y, DING X Y, ZHU L Q, et al.The identification of a B-cell epitope in bovine viral diarrhea virus (BVDV) core protein based on a mimotope obtained from a phage-displayed peptide library[J].Int J Biol Macromol, 2021, 183:2376-2386.
[19]	GIL L H V G, ANSARI I H, VASSILEV V, et al.The amino-terminal domain of bovine viral diarrhea virus Npro protein is necessary for alpha/beta interferon antagonism[J].J Virol, 2006, 80(2):900-911.
[20]	ZVRCHER C, SAUTER K S, SCHWEIZER M.Pestiviral Erns blocks TLR-3-dependent IFN synthesis by LL37 complexed RNA[J].Vet Microbiol, 2014, 174(3-4):399-408.
[21]	REN X, ZHANG S, GAO X T, et al.Experimental immunization of mice with a recombinant bovine enterovirus vaccine expressing BVDV E0 protein elicits a long-lasting serologic response[J].Virol J, 2020, 17(1):88.
[22]	GAO Y G, ZHAO X L, ZANG P, et al.Generation of the bovine viral diarrhea virus e0 protein in transgenic astragalus and its immunogenicity in sika deer[J].Evid Based Complement Alternat Med, 2014, 2014:372503.
[23]	MU Y, TEWS B A, LUTTERMANN C, et al.Interaction of pestiviral E1 and E2 sequences in dimer formation and intracellular retention[J].Int J Mol Sci, 2021, 22(14):7285.
[24]	RONECKER S, ZIMMER G, HERRLER G, et al.Formation of bovine viral diarrhea virus E1-E2 heterodimers is essential for virus entry and depends on charged residues in the transmembrane domains[J].J Gen Virol, 2008, 89(Pt 9):2114-2121.
[25]	EL OMARI K, IOURIN O, HARLOS K, et al.Structure of a pestivirus envelope glycoprotein E2 clarifies its role in cell entry[J].Cell Rep, 2013, 3(1):30-35.
[26]	LI Y, WANG J M, KANAI R, et al.Crystal structure of glycoprotein E2 from bovine viral diarrhea virus[J].Proc Natl Acad Sci U S A, 2013, 110(17):6805-6810.
[27]	WANG J M, LI Y, MODIS Y.Structural models of the membrane anchors of envelope glycoproteins E1 and E2 from pestiviruses[J].Virology, 2014, 454-455:93-101.
[28]	MU Y, RADTKE C, TEWS B A, et al.Characterization of membrane topology and retention signal of pestiviral glycoprotein E1[J].J Virol, 2021, 95(15):e00521-21.
[29]	WANG Z H, LIU M Y, ZHAO H R, et al.Induction of robust and specific humoral and cellular immune responses by bovine viral diarrhea virus virus-like particles (BVDV-VLPs) engineered with baculovirus expression vector system[J].Vaccines (Basel), 2021, 9(4):350.
[30]	MA X X, MA P, CHANG Q Y, et al.The analyses of relationships among nucleotide, synonymous codon and amino acid usages for E2 gene of bovine viral diarrhea virus[J].Gene, 2018, 660:62-67.
[31]	DONOFRIO G, BOTTARELLI E, SANDRO C, et al.Expression of bovine viral diarrhea virus glycoprotein E2 as a soluble secreted form in a Mammalian cell line[J].Clin Vaccine Immunol, 2006, 13(6):698-701.
[32]	MERWAISS F, PASCUAL M J, POMILIO M T, et al.A β-hairpin motif in the envelope protein E2 mediates receptor binding of bovine viral diarrhea virus[J].Viruses, 2021, 13(6):1157.
[33]	TAUTZ N, TEWS B A, MEYERS G.The molecular biology of pestiviruses[J].Adv Virus Res, 2015, 93:47-160.
[34]	郭妍婷.牛病毒性腹泻病毒离子通道蛋白p7的互作蛋白筛选及功能研究[D].乌鲁木齐:新疆农业大学, 2021.GUO Y T.Screening and functional research of interacting proteins of bovine viral diarrhea virus ion channel protein p7[D].Urumqi:Xinjiang Agricultural University, 2021.(in Chinese)
[35]	WALTHER T, FELLENBERG J, KLEMENS O, et al.Membrane topology of pestiviral nonstructural protein 2 and determination of the minimal autoprotease domain[J].J Virol, 2021, 95(11):e00154-21.
[36]	WALTHER T, BRUHN B, ISKEN O, et al.A novel NS3/4A protease dependent cleavage site within pestiviral NS2[J].J Gen Virol, 2021, 102(10).doi:10.1099/jgv.0.001666.
[37]	DUBRAU D, SCHWINDT S, KLEMENS O, et al.Determination of critical requirements for classical swine fever virus NS2-3-independent virion formation[J].J Virol, 2019, 93(18):e00679-19.
[38]	RIDPATH J F.Immunology of BVDV vaccines[J].Biologicals, 2013, 41(1):14-19.
[39]	LI Y, JIA Y, WEN K, et al.Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus[J].Vet Immunol Immunopathol, 2013, 151(3-4):331-336.
[40]	YUE S, ZHAO T, MA J Z, et al.Bovine viral diarrhea virus NS4B protein interacts with 2CARD of MDA5 domain and negatively regulates the RLR-mediated IFN-β production[J].Virus Res, 2021, 302:198471.
[41]	SUDA Y, MURAKAMI S, HORIMOTO T.Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells[J].Arch Virol, 2019, 164(1):255-260.
[42]	LI G Y, ADAM A, LUO H L, et al.An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses[J].npj Vaccines, 2019, 4:48.
[43]	BASHIR S, KOSSAREV A, MARTIN V C, et al.Deciphering the role of bovine viral diarrhea virus non-structural NS4B protein in viral pathogenesis[J].Vet Sci, 2020, 7(4):169.
[44]	XU J M, XIE X P, YE N, et al.Design, synthesis, and biological evaluation of substituted 4, 6-dihydrospiro[1, 2, 3] triazolo[4, 5-b] pyridine-7, 3'-indoline] -2', 5(3H)-dione analogues as potent NS4B inhibitors for the treatment of dengue virus infection[J].J Med Chem, 2019, 62(17):7941-7960.
[45]	ROTH C, CANTAERT T, COLAS C, et al.A modified mRNA vaccine targeting immunodominant NS epitopes protects against dengue virus infection in HLA class I transgenic mice[J].Front Immunol, 2019, 10:1424.
[46]	ZAHOOR M A, YAMANE D, MOHAMED Y M, et al.Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKβ-binding protein[J].J Gen Virol, 2010, 91(Pt 8):1939-1948.
[47]	CURTI E, JAEGER J.Residues Arg283, Arg285, and Ile287 in the nucleotide binding pocket of bovine viral diarrhea virus NS5B RNA polymerase affect catalysis and fidelity[J].J Virol, 2013, 87(1):199-207.
[48]	DUAN H, MA Z Q, XU L L, et al.A novel intracellularly expressed NS5B-specific nanobody suppresses bovine viral diarrhea virus replication[J].Vet Microbiol, 2020, 240:108449.
[49]	SCHWEIZER M, STALDER H, HASLEBACHER A, et al.Eradication of bovine viral diarrhoea (BVD) in cattle in switzerland:lessons taught by the complex biology of the virus[J].Front Vet Sci, 2021, 8:702730.
[50]	HE Y Q, RACZ R, SAYERS S, et al.Updates on the web-based VIOLIN vaccine database and analysis system[J].Nucleic Acids Res, 2014, 42(D1):D1124-D1132.
[51]	MOENNIG V, BECHER P.Pestivirus control programs:how far have we come and where are we going[J].Anim Health Res Rev, 2015, 16(1):83-87.
[52]	THOMAS C, YOUNG N J, HEANEY J, et al.Evaluation of efficacy of mammalian and baculovirus expressed E2 subunit vaccine candidates to bovine viral diarrhoea virus[J].Vaccine, 2009, 27(17):2387-2393.
[53]	MODY K T, MAHONY D, ZHANG J, et al.Silica vesicles as nanocarriers and adjuvants for generating both antibody and T-cell mediated immune resposes to bovine viral diarrhoea virus E2 protein[J].Biomaterials, 2014, 35(37):9972-9983.
[54]	SADAT S M A, SNIDER M, GARG R, et al.Local innate responses and protective immunity after intradermal immunization with bovine viral diarrhea virus E2 protein formulated with a combination adjuvant in cattle[J].Vaccine, 2017, 35(27):3466-3473.
[55]	DONOFRIO G, SARTORI C, FRANCESCHI V, et al.Double immunization strategy with a BoHV-4-vectorialized secreted chimeric peptide BVDV-E2/BoHV-1-gD[J].Vaccine, 2008, 26(48):6031-6042.
[56]	DONOFRIO G, FRANCESCHI V, CAPOCEFALO A, et al.Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4-based vaccine vector development[J].Clin Vaccine Immunol, 2009, 16(11):1675-1686.
[57]	CHOWDHURY S I, PANNHORST K, SANGEWAR N, et al.BoHV-1-vectored BVDV-2 subunit vaccine induces BVDV cross-reactive cellular immune responses and protects against BVDV-2 challenge[J].Vaccines (Basel), 2021, 9(1):46.
[58]	BHUYAN A A, MEMON A M, BHUIYAN A A, et al.The construction of recombinant Lactobacillus casei expressing BVDV E2 protein and its immune response in mice[J].J Biotechnol, 2018, 270:51-60.
[59]	JIA S, HUANG X N, LI H, et al.Immunogenicity evaluation of recombinant Lactobacillus casei W56 expressing bovine viral diarrhea virus E2 protein in conjunction with cholera toxin B subunit as an adjuvant[J].Microb Cell Fact, 2020, 19(1):186.
[60]	CAI D J, SONG Q J, DUAN C, et al.Enhanced immune responses to E2 protein and DNA formulated with ISA 61 VG administered as a DNA prime-protein boost regimen against bovine viral diarrhea virus[J].Vaccine, 2018, 36(37):5591-5599.
[61]	BELLIDO D, BAZTARRICA J, ROCHA L, et al.A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle[J].Transbound Emerg Dis, 2021, 68(6):3474-3481.
[62]	WANG S H, YANG G H, NIE J W, et al.Immunization with recombinant Erns-LTB fusion protein elicits protective immune responses against bovine viral diarrhea virus[J].Vet Microbiol, 2021, 259:109084.
[63]	WANG Y X, YANG G H, ZHANG L L, et al.Melatonin as immune potentiator for enhancing subunit vaccine efficacy against bovine viral diarrhea virus[J].Vaccines (Basel), 2021, 9(9):1039.
[64]	WANG S H, YANG G H, NIE J W, et al.Recombinant Erns-E2 protein vaccine formulated with MF59 and CPG-ODN promotes T cell immunity against bovine viral diarrhea virus infection[J].Vaccine, 2020, 38(22):3881-3891.
[65]	SANGEWAR N, HASSAN W, LOKHANDWALA S, et al.Mosaic bovine viral diarrhea virus antigens elicit cross-protective immunity in calves[J].Front Immunol, 2020, 11:589537.
[66]	WETZEL D, BARBIAN A, JENZELEWSKI V, et al.Bioprocess optimization for purification of chimeric VLP displaying BVDV E2 antigens produced in yeast Hansenula polymorpha[J].J Biotechnol, 2019, 306:203-212.
[67]	RIITHO V, WALTERS A A, SOMAVARAPU S, et al.Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens[J].Sci Rep, 2017, 7(1):13743.

牛病毒性腹泻病毒蛋白的免疫学特性以及相关疫苗研究进展

Research Progress on Immunological Characteristics of Bovine Viral Diarrhea Virus Protein and Vaccines

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 67

相关文章 15

编辑推荐

Metrics

本文评价

[1]	牟湘钰, 徐云若, 胡静怡, 周欣妍, 朱勇文. 家禽支链氨基酸营养需要研究进展[J]. 畜牧兽医学报, 2024, 55(1): 31-38.
[2]	郑先瑞, 卓明雪, 纪金丽, 蒋维虎, 邓在双, 张吉成, 田雅莉, 丁月云, 张晓东, 殷宗俊. 皖南黑猪不同生长阶段血清免疫指标及肠道菌群的特征分析[J]. 畜牧兽医学报, 2023, 54(9): 3770-3783.
[3]	龙美贞, 李浩运, 董玉慧, 南岳, 周向梅. 一例安德鲁貂尾尖软骨样脊索瘤的组织病理学观察及分析[J]. 畜牧兽医学报, 2023, 54(9): 3998-4002.
[4]	王姿月, 张子卉, 吴文学, 彭辰. 猴痘的诊断、预防和治疗[J]. 畜牧兽医学报, 2023, 54(8): 3195-3205.
[5]	钟华, 宋杉杉, 邵浣婷, 赵瑜, 康劲文, 吴瑶, 苏仁伟. 犬子宫蓄脓组织的转录组学分析[J]. 畜牧兽医学报, 2023, 54(8): 3383-3392.
[6]	王建东, 唐玉林, 王敏, 张宝锁, 杨富强, 高海慧, 于洋, 郭延生. 基于RNA-Seq技术研究枸杞多糖对环磷酰胺致雏鸡免疫抑制的拮抗机制[J]. 畜牧兽医学报, 2023, 54(8): 3519-3532.
[7]	焦广明, 吕英光, 桑金芳, 寇志鹏, 刘涛, 王月, 陆翔宇, 朴晨曦, 马亚军, 张建涛, 王洪斌. 脂肪间充质干细胞与甲泼尼龙联合用药对小型猪异体皮肤移植的影响[J]. 畜牧兽医学报, 2023, 54(8): 3533-3545.
[8]	陈鑫, 秦彤. mRNA疫苗及其在动物传染病中的研究展望[J]. 畜牧兽医学报, 2023, 54(7): 2732-2742.
[9]	赵婉莉, 曹棋棋, 杨悦, 邓昭举, 徐闯. 胃肠道菌群与黏膜免疫在围产期奶牛健康中的作用[J]. 畜牧兽医学报, 2023, 54(7): 2751-2760.
[10]	米慧, 彭灿, 贺志雄, 谭支良. 基于流式细胞术分选绵羊分泌型IgA包裹的消化道细菌[J]. 畜牧兽医学报, 2023, 54(7): 2924-2931.
[11]	程天印, 吴聪颖, 刘雨珂, 段德勇. 褐黄血蜱HSP70-b2及其类14-Mer肽的作用研究[J]. 畜牧兽医学报, 2023, 54(6): 2570-2580.
[12]	郑若愚, 任永军, 肖洁, 白鑫, 蒲家艳, 陈浩, 杨光友. 斯氏艾美耳球虫3-磷酸甘油醛脱氢酶重组蛋白对兔的免疫保护效果评价[J]. 畜牧兽医学报, 2023, 54(6): 2581-2595.
[13]	李雯乔, 李发弟, 王新基, 乐祥鹏. 家畜早期妊娠诊断的研究进展[J]. 畜牧兽医学报, 2023, 54(5): 1782-1791.
[14]	陈芳芳, 栗中华, 朱志伟, 李锦春, 刘翠艳. 恒定链的多功能研究新进展[J]. 畜牧兽医学报, 2023, 54(5): 1824-1833.
[15]	龙琴琴, 魏敏, 王雨婷, 文明, 庞峰. 羊口疮病毒与宿主的博弈:免疫应答与病毒免疫逃逸机制[J]. 畜牧兽医学报, 2023, 54(5): 1845-1853.