硫酸化党参多糖对H2O2致RAW264.7细胞损伤和小鼠急性酒精损伤的保护作用

doi:10.11843/j.issn.0366-6964.2022.06.028

摘要/Abstract

摘要： 本试验旨在比较党参多糖(CP)与硫酸化党参多糖(SCP)对H₂O₂诱导RAW264.7细胞急性损伤及小鼠急性酒精损伤的保护作用。采用H₂O₂诱导RAW264.7细胞急性损伤，检测不同浓度CP与SCP对RAW264.7细胞生长、吞噬功能及IL-2、IL-4、IL-10和IFN-γ水平的影响。随后体内建立小鼠急性酒精损伤模型，将90只4周龄雄性昆明小鼠随机均分为9组：正常对照组(B)、模型对照组(MO)、维生素C对照组(VC)、3个剂量的CP(CPL、CPM、CPH)与SCP(SCPL、SCPM、SCPH)组。检测各组对急性酒精损伤小鼠生长情况、血液生理指标、脏器指数、血清IL-2、IL-4、IL-10及IFN-γ水平的影响。结果显示，H₂O₂诱导RAW264.7细胞急性损伤后，与MO组相比，CP和SCP组细胞贴壁数量增加，细胞密度增大，37 ℃作用30、60、90 min后均能显著提高细胞吞噬能力(P < 0.05)，且SCP各组促进细胞增殖及吞噬作用优于CP各组。CP和SCP均能显著促进细胞分泌IL-2、IFN-γ(P < 0.05)，抑制IL-4的分泌(P < 0.05)，SCP各组对IL-4的抑制作用优于CP，其中SCPL组对IL-4的抑制效果最佳。小鼠急性酒精损伤模型中，与MO组相比，CP和SCP能显著改善小鼠血液生理指标(P < 0.05)，使血液中WBC和HGB恢复至正常水平，其中SCPL组效果最佳。CP和SCP还能改善小鼠血生化指标，显著提高血清中TC、LDH含量(P < 0.05)，降低TG含量(P < 0.05)。CP和SCP组较MO组小鼠血清中IL-2、IFN-γ水平显著升高(P < 0.05)，IL-4水平显著降低(P < 0.05)，其中SCP各组对IL-4的抑制作用最佳。综上所述，SCP可显著提高H₂O₂诱导RAW264.7细胞急性损伤后细胞吞噬能力，促进细胞分泌IL-2、IFN-γ水平，抑制IL-4分泌；改善酒精诱导的急性损伤小鼠的血液生理指标、血清生化指标和免疫因子，具有一定的保护作用，且SCP在一定条件下效果优于CP。

关键词: 党参多糖, 硫酸化, 急性损伤, 免疫, 细胞因子

Abstract: The aim of this study was to compare the immunoprotective effects of Codonopsis pilosula polysaccharide (CP) and sulphated Codonopsis pilosula polysaccharide (SCP) on acute injury induced by alcohol in mice and H₂O₂ in RAW264.7 cells. RAW264.7 cells was induced by H₂O₂ to build a cellular model of acute injury, and the effects of different concentrations of CP and SCP on the growth, phagocytosis, and IL-2, IL-4, IL-10, IFN-γ levels of RAW264.7 cells were detected. In addition, a mouse model of acute injury induced by alcohol was established in vivo. A total of 90 4-week-old Kunming male mice were randomly divided into 9 groups: blank group (B), model control group (MO), Vitamin C control group (VC), 3 doses of CP (CPL, CPM, CPH) and SCP (SCPL, SCPM, SCPH) groups. The effects of each group on the growth, blood physiological indexes, organ index, serum IL-2, IL-4, IL-10 and IFN-γ levels of acute injury mice were detected. The results showed that in the H₂O₂-induced cell inflammation model, compared with the model group, the number of adherent cells and the cell density in CP and SCP groups remarkably increased, and the phagocytosis ability of cells was significantly improved after 30, 60 and 90 min treatment at 37 ℃ (P < 0.05). Moreover, SCP groups showed better effects of promoting cell proliferation and phagocytosis. Both CP and SCP could significantly promote the secretion of IL-2 and IFN-γ (P < 0.05), and inhibit the secretion of IL-4 (P < 0.05). SCP groups had better inhibition effect on IL-4 than CP groups, and SCPL group had the best inhibition effect on IL-4. In acute injury model induced by alcohol, compared with model group, both CP and SCP could significantly improve the blood physiological indexes of mice (P < 0.05), and restore the WBC and HGB to normal levels, among which SCPL group had the best effect. CP and SCP could also improve serum biochemical indexes, significantly increase the contents of TC and LDH in serum (P < 0.05), and reduce the content of TG (P < 0.05). The serum levels of IL-2 and IFN-γ in CP and SCP groups were significantly increased (P < 0.05) compared with MO group, and the level of IL-4 was significantly decreased (P < 0.05), in which SCP groups had the best inhibition effect on IL-4. In conclusion, SCP significantly increased the phagocytosis ability of RAW264.7 cells after acute injury induced by H₂O₂, promoted the secretion of IL-2 and IFN-γ, and inhibited the secretion of IL-4. SCP, which had protection effect, can improve blood physiological indexes, serum biochemical indices and immune factors of acute injury mice induced by alcohol. Under certain conditions, the protective effect of SCP was better than CP.

Key words: Codonopsis pilosula polysaccharide, sulfate, acute injury, immune, cytokines

中图分类号:

S853.74

孙嘉琪, 杨诗靖, 谭盈, 白霖, 郭世宁, 石达友, 刘翠. 硫酸化党参多糖对H₂O₂致RAW264.7细胞损伤和小鼠急性酒精损伤的保护作用[J]. 畜牧兽医学报, 2022, 53(6): 1945-1957.

SUN Jiaqi, YANG Shijing, TAN Ying, BAI Lin, GUO Shining, SHI Dayou, LIU Cui. Protective Effect of Sulfated Codonopsis pilosula Polysaccharide on RAW264.7 Cell Damage Induced by H₂O₂ and Acute Alcohol Injury in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1945-1957.

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硫酸化党参多糖对H₂O₂致RAW264.7细胞损伤和小鼠急性酒精损伤的保护作用

Protective Effect of Sulfated Codonopsis pilosula Polysaccharide on RAW264.7 Cell Damage Induced by H₂O₂ and Acute Alcohol Injury in Mice

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