干扰素刺激基因对羊口疮病毒在OFTu细胞中增殖的抑制效应

doi:10.11843/j.issn.0366-6964.2022.02.025

摘要/Abstract

摘要： 羊口疮病毒（ORFV）是重要的人兽共患病病原，不仅严重危害养羊业，而且威胁人类健康。干扰素刺激基因（stimulator of interferon genes，STING）作为细胞的DNA感受器，在机体天然免疫中起重要作用。为探索STING在ORFV感染中的作用及其对病毒复制的影响，本研究构建了ORFV感染羊胚胎鼻甲细胞（OFTu）的模型，分析了ORFV感染细胞后对STING及其相关基因的动态表达，探索了STING基因在干扰表达和过表达状态下对ORFV在细胞上增殖的影响。结果表明，ORFV感染OFTu细胞后，STING、cGAS、TBK1、IRF3、IRF7、IL-6、IFN-β、IL-1β和TNF-α的转录明显升高。OFTu细胞过表达STING可导致RIG-1、DDX41、IFI16、IRF3、IRF7、IL-6、TNF-α、IFN-α和IFN-β等基因转录上调。OFTu细胞在STING过表达状态下感染ORFV可介导TBK-1、IRF3、IFN-β和TNF-α的转录升高，抑制ORFV的复制；在STING表达干扰的状态下，ORFV感染OFTu细胞降低了TBK-1、IRF3、IFN-β和TNF-α的转录，增加了ORFV的复制。这表明STING蛋白能够增强抗病毒细胞因子的表达，抑制ORFV在OFTu细胞中的增殖，研究结果为深入理解STING在羊口疮病毒感染和复制中的作用提供了科学的理论依据，也为深入探索ORFV感染和致病的分子机制提供了基础数据。

关键词: 羊口疮病毒, STING, 感染, 复制

Abstract: As an important zoonotic virus, orf virus (ORFV) not only seriously affects the sheep breeding, but also threatens human health. Stimulator of interferon genes (STING), as DNA sensor of the cell, plays an important role in innate immunity of the host. In order to explore the role of STING in infection and replication of ORFV, cell model of ORFV infection of ovine fetal turbinate cells (OFTu) was constructed and dynamic expression of STING and related genes after ORFV infection in cells were analyzed. The effects of STING on ORFV proliferation were investigated under the state of interference expression and overexpression in OFTu cells. The results showed that the transcription of STING, cGAS, TBK1, IRF3, IRF7, IL-6, IFN-β, IL-1β and TNF-α were significantly increased after OFTu cells infected with ORFV. OFTu cells with STING overexpression resulted in up-regulated transcription of RIG-Ⅰ,DDX41, IFI16, IRF3, IRF7, IL-6, TNF-α, IFN-α and IFN-β.Infection of OFTu cells with STING overexpression can increase the expression of TBK-1, IRF3, IFN-β and TNF-α genes and inhibit the replication of ORFV. While, infection of OFTu cells with the interference of STING expression can decrease the transcription of TBK-1, IRF3, IFN-β and TNF-α and increase the replication of ORFV. These results indicated that STING protein can enhance the expression of antiviral cytokines and inhibit the proliferation of ORFV in OFTu cells. The results provide scientific theoretical basis for further understanding the role of STING in infection and replication of ORFV, and provide basic data for further exploration of the molecular mechanism of ORFV infection and pathogenicity.

Key words: orf virus, STING, infection, replication

中图分类号:

S852.659.1

陈永杰, 刘健新, 李慧子, 钟文霞, 李保建, 皮墨霖, 宁章勇. 干扰素刺激基因对羊口疮病毒在OFTu细胞中增殖的抑制效应[J]. 畜牧兽医学报, 2022, 53(2): 588-596.

CHEN Yongjie, LIU Jianxin, LI Huizi, ZHONG Wenxia, LI Baojian, PI Molin, NING Zhangyong. Inhibitory Effect of Stimulator of Interferon Genes on the Proliferation of Orf Virus in OFTu Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 588-596.

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