母牛安静发情鉴定技术概况及影响因素分析

doi:10.11843/j.issn.0366-6964.2021.04.002

摘要/Abstract

摘要： 安静发情母牛无明显发情行为，但具有正常卵泡发育、排卵过程，尚无理想的发情鉴定方法，常常错过人工输精最佳时机，导致产犊间隔延长，饲养成本增加。为此，本文综述了安静发情的鉴定方法概况及母牛安静发情的可能诱因。并指出，科学的管理及合理的营养可尽量减少母牛安静发情。此外，深入挖掘粪便、尿液、乳汁等发情标志物，研究其化合物在发情周期中的变化规律，并借助物联网、生物信息等技术，研发更精准、高效的发情鉴定技术，就有可能彻底突破安静发情鉴定技术瓶颈。

关键词: 安静发情, 发情检测, 卵泡发育, 母牛

Abstract: Although cows in silent estrus don’t show overt estrus behaviors, the normal follicular development and ovulation process can also be detected. The cows in silent estrus often miss the best opportunity for artificial insemination since no ideal oestrous identification method, which leads to a longer calving interval and the increase of feeding cost. Therefore, the identification method and the possible causes of silent estrus in cows were reviewed. It was pointed out that scientific management and reasonable nutrition could decrease the occurrence of silent estrus in cows. In addition, the estrus markers in feces, urine and milk should be given an deep study to understand the various physiological changes during the oestrous cycle, combining these physiological parameters and the internet, bioinformatics and other technologies, which will enable us to develop more accurate and efficient estrus detection technology, and thoroughly overcome the technical bottleneck of silent estrus identification.

Key words: silent estrus, estrus detection, follicular development, cow

中图分类号:

S814.1

周正义, 田莉, 田宏志, 李茹意, 段晨莹, OBIANWUNA Uchechukwu Edna, 吕丽华, 王栋. 母牛安静发情鉴定技术概况及影响因素分析[J]. 畜牧兽医学报, 2021, 52(4): 862-871.

ZHOU Zhengyi, TIAN Li, TIAN Hongzhi, LI Ruyi, DUAN Chenying, OBIANWUNA Uchechukwu Edna, Lü Lihua, WANG Dong. Analysis of Identification Technology and Influence Factors on Silent Estrus of Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 862-871.

参考文献 72

[1]	韩志强,王海军,赵家平,等.动物发情鉴定技术的研究进展[J].畜牧兽医学报,2018,49(10):2086-2091.HAN Z Q,WANG H J,ZHAO J P,et al.Research progress of estrus identification technology in animals[J].Acta Veterinaria et Zootechnica Sinica,2018,49(10):2086-2091.(in Chinese)
[2]	李鸿斌.浅谈对发情母牛症状的观察[J].畜禽业,2019,30(5):24.LI H B.Analysis observation on symptoms of oestrus cows without depth[J].Livestock and Poultry Industry,2019,30(5):24.(in Chinese)
[3]	王锋.动物繁殖学[M].北京:中国农业大学出版社,2012.WANG F.Animal reproduction[M].Beijing:China Agricultural University Press,2012.(in Chinese)
[4]	YÁNIZ J L,LÓPEZ-GATIUS F,BECH-SÀBAT G,et al.Relationships between milk production,ovarian function and fertility in high-producing dairy herds in north-eastern Spain[J].Reprod Domest Anim,2008,43(S4):38-43.
[5]	ESSLEMONT R J,KOSSAIBATI M A,ALLCOCK J,et al.Economics of fertility in dairy cows[J].BSAP Occas Publ,2001,26(1):19-29.
[6]	RORIE R W,BILBY T R,LESTER T D.Application of electronic estrus detection technologies to repro-ductive management of cattle[J].Theriogenology,2002, 57(1):137-148.
[7]	MAYO L M,SILVIA W J,RAY D L,et al.Automated estrous detection using multiple commercial precision dairy monitoring technologies in synchronized dairy cows[J].J Dairy Sci,2019,102(3):2645-2656.
[8]	李蓝祁.奶牛繁殖周期体温与活动量自动检测及变化规律研究[D].长沙:湖南农业大学,2018.LI L Q.Study on automatic detection and variation of body temperature and activity in dairy cows during reproductive cycle[D].Changsha:Hunan Agricultural University,2018.(in Chinese)
[9]	CHOUDHARY S,KAMBOJ M L.Effect of bull biostimulation on the oestrous behaviour of pubertal Sahiwal (Bos indicus) heifers[J].Anim Reprod Sci,2019,209:106149.
[10]	REITH S,HOY S.Review:Behavioral signs of estrus and the potential of fully automated systems for detection of estrus in dairy cattle[J].Animal,2018,12(2):398-407.
[11]	SCHAMS D,SCHALLENBERGER E,HOFFMANN B,et al.The oestrous cycle of the cow:hormonal parameters and time relationships concerning oestrus,ovulation,and electrical resistance of the vaginal mucus[J].Acta Endocrinol (Copenh),1977, 86(1):180-192.
[12]	HOJO T,SAKATANI M,TAKENOUCHI N,et al.Efficiency of a pedometer device for detecting estrus in standing heat and silent heat in Japanese Black cattle[J].Anim Sci J,2018,89(8):1067-1072.
[13]	GRIMM K,HAIDN B,ERHARD M,et al.New insights into the association between lameness,behavior,and performance in Simmental cows[J].J Dairy Sci,2019,102(3):2453-2468.
[14]	张春梅,席丽,李志强,等.奶牛的发情鉴定方法比较[J].当代畜禽养殖业,2019(1):4-7.ZHANG C M,XI L,LI Z Q,et al.Comparison of methods for identification of oestrus in dairy cows[J].Modern Animal Husbandry,2019(1):4-7.(in Chinese)
[15]	SAKATANI M,TAKAHASHI M,TAKENOUCHI N,et al.The efficiency of vaginal temperature measurement for detection of estrus in Japanese Black cows[J].J Reprod Dev,2016,62(2):201-207.
[16]	GIL Z,SZAREK J,KURAL J,et al.Detection of silent oestrus in dairy cows by milk temperature measurement[J].Anim Sci,1997, 65(1):25-29.
[17]	马吉锋,王建东,李艳艳,等.UCOWS奶牛发情监测系统检测奶牛发情效果的研究[J].黑龙江畜牧兽医,2014(16):35-36.MA J F,WANG J D,LI Y Y,et al.Study on oestrus detection effect of UCOWS cow estrus monitoring system[J].Heilongjiang Animal Science and Veterinary Medicine,2014(16):35-36.(in Chinese)
[18]	GARCIA-ISPIERTO I,LÓPEZ-GATIUS F.A three-day PGF2α plus eCG-based fixed-time AI protocol improves fertility compared with spontaneous estrus in dairy cows with silent ovulation[J].J Reprod Dev,2013,59(4):393-397.
[19]	雪雅妮.B超检查奶牛同期发情和超数排卵期卵巢发育研究[J].畜牧兽医科技信息,2016(7):36-37.XUE Y N.Study on ovarian development during estrus synchronization and superovulation by B-ultrasound in dairy cows[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2016(7):36-37.(in Chinese)
[20]	WHITE I R,RUSSEL A J,WRIGHT I A,et al.Real-time ultrasonic scanning in the diagnosis of pregnancy and the estimation of gestational age in cattle[J].Vet Rec,1985,117(1):5-8.
[21]	PIETERSE M C,SZENCI O,WILLEMSE A H,et al.Early pregnancy diagnosis in cattle by means of linear-array real-time ultrasound scanning of the uterus and a qualitative and quantitative milk progesterone test[J].Theriogenology,1990,33(3):697-707.
[22]	RICCETTI L,SPERDUTI S,LAZZARETTI C,et al.The cAMP/PKA pathway:steroidogenesis of the antral follicular stage[J].Minerva Ginecol,2018,70(5):516-524.
[23]	许钦彬.奶牛繁殖生理的重要性[J].畜牧兽医科技信息,2018(7):73.XU Q B.The importance of reproductive physiology in dairy cows[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2018(7):73.(in Chinese)
[24]	田宏志,刘江静,陈晓丽,等.奶牛生殖激素和体温的性周期变化及调控机制研究进展[J].中国畜牧杂志,2019,55(2):27-32.TIAN H Z,LIU J J,CHEN X L,et al.Research progress on sexual cycle changes and regulatory mechanisms of reproduction hormone and body temperature in dairy cows[J].Chinese Journal of Animal Science,2019,55(2):27-32.(in Chinese)
[25]	邓立新,贾翔征,贺丛,等.B超检查奶牛同期发情和超数排卵期卵巢发育研究[J].畜牧兽医杂志,2004,23(1):1-4.DENG L X,JIA X Z,HE C,et al.A primary study on the ovary checking by B-ultrascanerin cow's synchroestrus and superovulation[J].Journal of Animal Science and Veterinary Medicine,2004,23(1):1-4.(in Chinese)
[26]	赵善江,童伟,王现军,等.定时人工授精技术及其对奶牛繁殖性能的影响[J].黑龙江动物繁殖,2020,28(2):29-38.ZHAO S J,TONG W,WANG X J,et al.Effects of timed artificial insemination on the reproductive performances of dairy cattle[J].Heilongjiang Journal of Animal Reproduction,2020,28(2):29-38.(in Chinese)
[27]	REESE S T,PEREIRA M H C,EDWARDS J L,et al.Pregnancy diagnosis in cattle using pregnancy associated glycoprotein concentration in circulation at day 24 of gestation[J].Theriogenology,2018,106:178-185.
[28]	BLACHE D,BATAILLER M,FABRE-NYS C,et al.Oestrogen receptors in the preoptico-hypothalamic continuum:immunohistochemical study of the distribution and cell density during induced oestrous cycle in ovariectomized ewe[J].J Neuroendocrinol, 1994,6(3):329-339.
[29]	INOUE S,YANG R Z,TANTRY A,et al.Periodic remodeling in a neural circuit governs timing of female sexual behavior[J].Cell, 2019,179(6):1393-1408.
[30]	RANASINGHE R M S B K,NAKAO T,YAMADA K,et al.Silent ovulation,based on walking activity and milk progesterone concentrations,in Holstein cows housed in a free-stall barn[J].Theriogenology,2010,73(7):942-949.
[31]	张仁森,刘秋月,胡文萍,等.家畜生殖激素测定方法研究进展[J].家畜生态学报,2020,41(11):1-5.ZHANG R S,LIU Q Y,HU W P,et al.Research progress on reproductive hormones determination in livestock[J].Acta Ecologiae Animalis Domastici,2020,41(11):1-5.(in Chinese)
[32]	BENABDELAZIZ A,BOUDJEMAI S,KHELILI R,et al.Development of in-house RIA kit for pro-gesterone in cow's skim milk[J].J Immunoassay Immunochem,2020,41(2):195-207.
[33]	KÄPPEL N D,PRÖLL F,GAUGLITZ G,et al.Development of a TIRF-based biosensor for sensitive detection of progesterone in bovine milk[J].Biosens Bioelectron,2007,22(9-10):2295-2300.
[34]	MAATJE K,ROSSING W.Detecting oestrus by measuring milk temperatures of dairy cows during milking[J].Liv Produc Sci,1976,3(1):85-89.
[35]	FORDHAM D P,ROWLINSON P,MCCARTHY T T,et al.Oestrus detection in dairy cows by milk temperature measurement[J]. Res Vet Sci,1988,44(3):366-374.
[36]	MCARTHUR A J,EASDON M P,GREGSON K.Milk temperature and detection of oestrus in dairy cattle[J].J Agric Eng Res, 1992,51:29-46.
[37]	FORDHAM D P,MCCARTHY T T,ROWLINSON P,et al.An evaluation of milk temperature measurement for detecting oestrus in dairy cattle.I factors affecting measurement of milk temperature[J].Vet Res Commun,1987,11(4):367-379.
[38]	寇红祥,赵福平,任康,等.奶牛体温与活动量检测及变化规律研究进展[J].畜牧兽医学报,2016,47(7):1306-1315.KOU H X,ZHAO F P,REN K,et al.The progress on detection method and the regularities of body temperature and activities in dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2016,47(7):1306-1315.(in Chinese)
[39]	ALI H E S,KITAHARA G,TAMURA Y,et al.Presence of a temperature gradient among genital tract portions and the thermal changes within these portions over the estrous cycle in beef cows[J].J Reprod Dev,2012,59(1):59-65.
[40]	WALTON J S,KING G J.Indicators of estrus in Holstein cows housed in tie stalls[J].J Dairy Sci,1986,69(11):2966-2973.
[41]	FISHER A D,MORTON R,DEMPSEY J M A,et al.Evaluation of a new approach for the estimation of the time of the LH surge in dairy cows using vaginal temperature and electrodeless conductivity measure-ments[J].Theriogenology,2008,70(7):1065-1074.
[42]	SUTHAR V S,BURFEIND O,BONK S,et al.Endogenous and exogenous progesterone influence body temperature in dairy cows[J].J Dairy Sci,2012,95(5):2381-2389.
[43]	李小俊,王振玲,陈晓丽,等.奶牛体温变化规律及繁殖应用研究进展[J].畜牧兽医学报,2016,47(12):2331-2341.LI X J,WANG Z L,CHEN X L,et al.Study progress on the rule of body temperature and its application in reproduction of dairy cattle[J].Acta Veterinaria et Zootechnica Sinica,2016,47(12):2331-2341.(in Chinese)
[44]	KUMAR K R,ARCHUNAN G,JEYARAMAN R,et al.Chemical characterization of bovine urine with special reference to oestrus[J].Vet Res Commun,2000,24(7):445-454.
[45]	SANKAR R,ARCHUNAN G.Identification of putative pheromones in bovine (Bos taurus) faeces in relation to estrus detection[J]. Anim Reprod Sci,2008,103(1-2):149-153.
[46]	BŪDA V,MOZ Ū RAITIS R,KUTRA J,et al.p-Cresol:a sex pheromone component identified from the estrous urine of mares[J].J Chem Ecol,2012,38(7):811-813.
[47]	GUILLAUME D,MOUSSU C,DE GEOFFROY F,et al.Olfactory stimulation or inhibition of sexual behavior of stallions in non-breeding season[J].Physiol Behav,2018,186:1-9.
[48]	KEKAN P M,INGOLE S D,SIRSAT S D,et al.The role of pheromones in farm animals-A review[J].Agric Rev,2017,38(2):83-93.
[49]	SELVAM R M,ONTERU S K,NAYAN V,et al.Exploration of Luteinizing hormone in murrah buffalo (Bubalus bubalis) urine:Extended surge window opens door for estrus prediction[J].Gen Comp Endocrinol,2017,251:121-126.
[50]	SRINIVASAN M,MUTHUKUMAR S,SAIBABA G,et al.Salivary luteinizing hormone:An open window to detect oestrous period in buffalo[J].Reprod Domest Anim,2020,55(5):647-651.
[51]	FISCHER-TENHAGEN C,WETTERHOLM L,TENHAGEN B A,et al.Training dogs on a scent platform for oestrus detection in cows[J].Appl Anim Behav Sci,2011,131(1-2):63-70.
[52]	FISCHER-TENHAGEN C,TENHAGEN B A,HEUWIESER W,et al.Short communication:Ability of dogs to detect cows in estrus from sniffing saliva samples[J].J Dairy Sci,2013,96(2):1081-1084.
[53]	SANGSRITAVONG S,COMBS D K,SARTORI R,et al.High feed intake increases liver blood flow and metabolism of progesterone and estradiol-17β in dairy cattle[J].J Dairy Sci,2002,85(11):2831-2842.
[54]	赵明礼,张珅,郝海生,等.同期发情和同期排卵-定时输精技术在奶牛繁殖中的应用[J].中国畜牧杂志,2015,51(17):80-86.ZHAO M L,ZHANG K,HAO H S,et al.Application of Estrus synchronization and ovsynch-timed artificial insemination in dairy cattle[J].Chinese Journal of Animal Science,2015,51(17):80-86.(in Chinese)
[55]	李富宽,杨燕,吕慎金.影响母畜发情因素及其内分泌机理的研究进展[J].中国畜牧杂志,2017,53(8):10-14.LI F K,YANG Y,LV S J.Research progress on influencing factors of estrous behavior and endocrine mechanism in female Livestocks[J].Chinese Journal of Animal Science,2017,53(8):10-14.(in Chinese)
[56]	张军,祝伟,矫正.浅谈母牛的安静发情[J].黑龙江动物繁殖,2009,17(2):27-28.ZHANG J,ZHU W,JIAO Z.Discuss the silent estrus of cows without depth[J].Heilongjiang Journal of Animal Reproduction,2009, 17(2):27-28.(in Chinese)
[57]	李艳萍.提高奶牛繁殖成活率的技术要点[J].吉林畜牧兽医,2018,39(5):52,54.LI Y P.Technical points of improving the survival rate of cow reproduction[J].Jilin Animal Husbandry and Veterinary Medicine, 2018,39(5):52,54.(in Chinese)
[58]	DOBSON H,FERGANI C,ROUTLY J E,et al.Effects of stress on reproduction in ewes[J].Anim Reprod Sci,2012,130(3-4):135-140.
[59]	WHISNANT C S,KISER T E,THOMPSON F N,et al.Effect of nutrition on the LH response to calf removal and GnRH[J]. Theriogenology, 1985,24(5):565-573.
[60]	张泰彪,朱新荣.荷斯坦牛血液中矿物质含量与常见疾病发病关系的研究[J].中国奶牛,2020(3):30-33.ZHANG T B,ZHU X R.Study on the relationship between blood mineral content and common diseases in Holstein cattle[J].China Dairy Cattle,2020(3):30-33.(in Chinese)
[61]	王胜强.母牛产后瘫痪的原因及综合防治措施[J].甘肃畜牧兽医,2018,48(1):59-60.WANG S Q.Causes of postpartum paralysis of cows and comprehensive control measures[J].Gansu Animal and Veterinary Sciences, 2018,48(1):59-60.(in Chinese)
[62]	吕咸坤.家畜缺乏微量元素铜、锌、锰的症状及防治[J].饲料博览,2017(5):58.LV X K.Symptoms and control of livestock lacking trace elements Cu,Zn and Mn[J].Feed Review,2017(5):58.(in Chinese)
[63]	张锋,张江,白云龙,等.卵巢静止奶牛血液矿物质及生化指标变化研究[J].黑龙江畜牧兽医,2020(11):54-56,62.ZHANG F,ZHANG J,BAI Y L,et al.Study on the changes of blood mineral and biochemical indexes in inactive ovarian dairy cows[J].Heilongjiang Animal Science and Veterinary Medicine,2020(11):54-56,62.(in Chinese)
[64]	胡文敏,张岭,李林子,等.雌激素及其受体在脂肪代谢中的作用[J].中国新药杂志,2016,25(11):1253-1257.HU W M,ZHANG L,LI L Z,et al.Effect of estrogen and its receptor in fat metabolism[J].Chinese Journal of New Drugs,2016,25(11):1253-1257.(in Chinese)
[65]	王懿,刘彩玲.雌激素和雌激素受体在脂肪组织中的作用[J].国外医学:妇幼保健分册,2003,14(2):94-95.WANG Y,LIU C L.The role of estrogen and estrogen receptor in adipose tissue[J].Foreign Medical Sciences:Section of Maternal and Child Health,2003,14(2):94-95.(in Chinese)
[66]	姬凯东.犊牛腹泻的病因及治疗措施[J].畜牧兽医科技信息,2019(9):87.JI K D.Etiology and treatment of calf diarrhea[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2019(9):87.(in Chinese)
[67]	KYLE S D,CALLAHAN C J,ALLRICH R D,et al.Effect of progesterone on the expression of estrus at the first postpartum ovulation in dairy cattle[J].J Dairy Sci,1992,75(6):1456-1460.
[68]	宋恩亮,谭秀文,刘晓牧,等.鲁西黄牛妊娠周期血清生殖及相关激素变化规律研究[J].中国牛业科学,2010,36(3):5-8.SONG E L,TAN X W,LIU X M,et al.Serum concentrations of procreation and correlative hormone during the gestation cycle in Luxi-yellow cattle[J].China Cattle Science,2010,36(3):5-8.(in Chinese)
[69]	LÓPEZ-GATIUS F,MIRZAEI A,SANTOLARIA P,et al.Factors affecting the response to the specific treatment of several forms of clinical anestrus in high producing dairy cows[J].Theriogenology,2008,69(9):1095-1103.
[70]	POLSKY L,MADUREIRA A M L,FILHO E L D,et al.Association between ambient temperature and humidity,vaginal temperature,and automatic activity monitoring on induced estrus in lactating cows[J].J Dairy Sci,2017,100(10):8590-8601.
[71]	ISMAEL A,STRANDBERG E,BERGLUND B,et al.Seasonality of fertility measured by physical activity traits in Holstein cows.[J].J Dairy Sci,2016,99(4):2837-2848.
[72]	GELEZ H,FABRE-NYS C.The "male effect" in sheep and goats:a review of the respective roles of the two olfactory systems[J].Horm Behav,2004,46(3):257-271.