褪黑素对镉致鸭大脑皮质毒性损伤的保护作用

doi:10.11843/j.issn.0366-6964.2021.010.026

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (10): 2953-2959.doi: 10.11843/j.issn.0366-6964.2021.010.026

褪黑素对镉致鸭大脑皮质毒性损伤的保护作用

张文华^1,2, 闻双全^1,2, 王莉^1,2, 徐明畅^1,2, 马勇刚^1,2, 邹辉^1,2, 顾建红^1,2, 刘学忠^1,2, 卞建春^1,2, 刘宗平^1,2, 袁燕^1,2*

1. 扬州大学兽医学院, 扬州 225009;
2. 江苏高校动物重要疫病与人畜共患病防控协同创新中心, 扬州 225009

收稿日期:2021-03-26 出版日期:2021-10-23 发布日期:2021-10-27
通讯作者: 袁燕,主要从事动物营养代谢病与中毒病研究,E-mail:yuanyan@yzu.edu.cn,Tel:0514-87979042
作者简介:张文华(1996-),男,河南漯河人,硕士生,主要从事动物营养代谢病与中毒病研究,E-mail:whz704@163.com
基金资助:
国家自然科学基金（31772808）；江苏高校优势学科建设工程资助项目（PAPD）；扬州大学优秀青年骨干教师资助；扬州大学校教改课题（YZUJX2019—51C）

Protective Effect of Melatonin on Toxic Damage Caused by Cadmium in Cerebral Cortex of Duck

ZHANG Wenhua^1,2, WEN Shuangquan^1,2, WANG Li^1,2, XU Mingchang^1,2, MA Yonggang^1,2, ZOU Hui^1,2, GU Jianhong^1,2, LIU Xuezhong^1,2, BIAN Jianchun^1,2, LIU Zongping^1,2, YUAN Yan^1,2*

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China

Received:2021-03-26 Online:2021-10-23 Published:2021-10-27

摘要/Abstract

摘要： 为探究褪黑素对镉致鸭大脑皮质毒性损伤的保护作用，本试验将16只20日龄高邮鸭随机分为4组，分别为对照组、褪黑素组、镉组、镉与褪黑素共处理组。对照组鸭自由采食饮水；褪黑素组鸭自由饮用含有0.2 mg·L^-1褪黑素的水；镉组鸭自由采食拌有2 mg·kg^-1氯化镉的饲料；镉与褪黑素共处理组鸭自由饮用含有0.2 mg·L^-1褪黑素水的同时自由采食拌有2 mg·kg^-1氯化镉的饲料。60 d后，剖检并采集鸭大脑皮质。比色法检测大脑皮质中丙二醛（MDA）和总抗氧化能力（T-AOC）的水平，ELISA法检测肿瘤坏死因子（TNF-α）、白介素-1β（IL-1β）的含量，免疫组化染色观察Nrf2核转位，免疫印迹法检测Nrf2、HO-1的蛋白表达。结果显示，与对照组相比，镉组鸭大脑皮质发生明显Nrf2核转位，T-AOC水平极显著降低（P<0.01），MDA、TNF-α、IL-1β含量和Nrf2、HO-1蛋白表达量极显著升高（P<0.01）；与镉组相比，镉与褪黑素共处理组鸭大脑皮质Nrf2核转位减少，T-AOC水平显著升高（P<0.05），MDA、TNF-α、IL-1β含量和Nrf2、HO-1蛋白表达量显著或极显著降低（P<0.05或P<0.01）。综上，褪黑素对镉所致的鸭大脑皮质毒性损伤具有一定的保护作用。

关键词: 褪黑素, 镉, 鸭, 大脑皮质, 毒性损伤

Abstract: In order to investigate the protective effect of melatonin on toxic damage caused by cadmium in cerebral cortex of duck, sixteen GaoYou ducks were randomly allocated into 4 groups:control group, melatonin-treated group, cadmium-treated group, cadmium and melatonin co-treated group. The ducks in the control group were free to eat and drink. The ducks in the melatonin group received drinking water containing 0.2 mg·L^-1 melatonin. The ducks in the cadmium group received feeding mixed with 2 mg·kg^-1 cadmium chloride. The ducks in the cadmium and melatonin co-treated group received drinking water containing 0.2 mg·L^-1 melatonin plus feeding mixed with 2 mg·kg^-1 cadmium chloride. After 60 d, the cerebral cortices of ducks were dissected and collected. The levels of malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in cerebral cortex of duck were measured by colorimetry, the contents of tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were detected by ELISA, the nuclear translocation of Nrf2 was observed by immunohistochemical staining, the protein expression of Nrf2 and HO-1 was detected by Western blot. The results showed that compared with the control group, the Nrf2 nuclear translocation was obvious, the level of T-AOC showed extremely significant decrease (P<0.01), the contents of MDA, TNF-α, IL-1β, the protein expression of Nrf2 and HO-1 showed extremely significant increase (P<0.01) in the cerebral cortex of duck in cadmium-treated group. Compared with the cadmium-treated group, the Nrf2 nuclear translocation was decreased, the level of T-AOC showed significant increase (P<0.05), the contents of MDA, TNF-α, and IL-1β, the protein expression of Nrf2 and HO-1 showed significant or extremely significant decrease (P<0.05 or P<0.01) in the cerebral cortex of duck in cadmium and melatonin co-treated group. The results indicate that melatonin has a protective effect on toxic damage caused by cadmium in cerebral cortex of duck.

Key words: melatonin, cadmium, duck, cerebral cortex, toxic damage

中图分类号:

S858.326.9

张文华, 闻双全, 王莉, 徐明畅, 马勇刚, 邹辉, 顾建红, 刘学忠, 卞建春, 刘宗平, 袁燕. 褪黑素对镉致鸭大脑皮质毒性损伤的保护作用[J]. 畜牧兽医学报, 2021, 52(10): 2953-2959.

ZHANG Wenhua, WEN Shuangquan, WANG Li, XU Mingchang, MA Yonggang, ZOU Hui, GU Jianhong, LIU Xuezhong, BIAN Jianchun, LIU Zongping, YUAN Yan. Protective Effect of Melatonin on Toxic Damage Caused by Cadmium in Cerebral Cortex of Duck[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2953-2959.

参考文献 22

[1]	GENCHI G,SINICROPI M S,LAURIA G,et al.The effects of cadmium toxicity[J].Int J Environ Res Public Health,2020,17(11):3782.
[2]	MÉNDEZ-ARMENTA M,RÍOS C.Cadmium neurotoxicity[J].Environ Toxicol Pharmacol,2007,23(3):350-358.
[3]	OSAMA A,ZHANG J M,YAO J,et al.Nrf2:a dark horse in Alzheimer's disease treatment[J].Ageing Res Rev,2020,64:101206.
[4]	HOSSEIN-KHANNAZER N,AZIZI G,ESLAMI S,et al.The effects of cadmium exposure in the induction of inflammation[J].Immunopharmacol Immunotoxicol,2020,42(1):1-8.
[5]	REITER R J,MAYO J C,TAN D X,et al.Melatonin as an antioxidant:under promises but over delivers[J].J Pineal Res,2016,61(3):253-278.
[6]	CAO Z W,FANG Y L,LU Y H,et al.Melatonin alleviates cadmium-induced liver injury by inhibiting the TXNIP-NLRP3 inflammasome[J].J Pineal Res,2017,62(3):e12389.
[7]	KNANI L,BARTOLINI D,KECHICHE S,et al.Melatonin prevents cadmium-induced bone damage:First evidence on an improved osteogenic/adipogenic differentiation balance of mesenchymal stem cells as underlying mechanism[J].J Pineal Res,2019,67(3):e12597.
[8]	YANG Q L,ZHU J,LUO X Y,et al.Melatonin attenuates cadmium-induced ovulatory dysfunction by suppressing endoplasmic reticulum stress and cell apoptosis[J].Reprod Biol Endocrinol,2019,17(1):61.
[9]	TSIKAS D.Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples:Analytical and biological challenges[J].Anal Biochem,2017,524:13-30.
[10]	闻双全,赵世文,陈洁,等.葛根素对镉暴露致大鼠大脑皮质氧化损伤的保护效应[J].畜牧与兽医,2018,50(12):104-107.WEN S Q,ZHAO S W,CHEN J,et al.Protective effects of puerarin on oxidative damage caused by cadmium exposure in cerebral cortex of rats[J].Animal Husbandry & Veterinary Medicine,2018,50(12):104-107.(in Chinese)
[11]	张萌萌.钼镉联合诱导对鸭脾脏氧化损伤及其相关基因表达的影响[D].南昌:江西农业大学,2016.ZHANG M M.The co-induced effects of molybdenum and cadmium on oxidative damage and related genes expression in duck spleen[D].Nanchang:Jiangxi Agricultural University,2016.(in Chinese)
[12]	TAGUCHI K,MOTOHASHI H,YAMAMOTO M.Molecular mechanisms of the Keap1-Nrf2 pathway in stress response and cancer evolution[J].Genes Cells,2011,16(2):123-140.
[13]	MOTOHASHI H,O'CONNOR T,KATSUOKA F,et al.Integration and diversity of the regulatory network composed of Maf and CNC families of transcription factors[J].Gene,2002,294(1-2):1-12.
[14]	SUZUKI T,YAMAMOTO M.Molecular basis of the Keap1-Nrf2 system[J].Free Radic Biol Med,2015,88:93-100.
[15]	LOBODA A,DAMULEWICZ M,PYZA E,et al.Role of Nrf2/HO-1 system in development,oxidative stress response and diseases:an evolutionarily conserved mechanism[J].Cell Mol Life Sci,2016,73(17):3221-3247.
[16]	TANG K K,LIU X Y,WANG Z Y,et al.Trehalose alleviates cadmium-induced brain damage by ameliorating oxidative stress,autophagy inhibition,and apoptosis[J].Metallomics,2019,11(12):2043-2051.
[17]	何刘,李慧,周厚继,等.槲皮素通过影响Nrf2-Keap1信号通路减缓镉中毒大鼠的生殖机能损伤[J].畜牧兽医学报,2018,49(5):1037-1046.HE L,LI H,ZHOU H J,et al.Protective effects of quercetin on the reproductive injury induced by cadmium through the Nrf2-Keap1 signaling pathway in rats[J].Acta Veterinaria et Zootechnica Sinica,2018,49(5):1037-1046.(in Chinese)
[18]	ALMEER R S,KASSAB R B,ALBASHER G I,et al.Royal jelly mitigates cadmium-induced neuronal damage in mouse cortex[J].Mol Biol Rep,2019,46(1):119-131.
[19]	LI T Q,YU H R,SONG Y Y,et al.Protective effects of Ganoderma triterpenoids on cadmium-induced oxidative stress and inflammatory injury in chicken livers[J].J Trace Elem Med Biol,2019,52:118-125.
[20]	SHAGIRTHA K,MUTHUMANI M,PRABU S M.Melatonin abrogates cadmium induced oxidative stress related neurotoxicity in rats[J].Eur Rev Med Pharmacol Sci,2011,15(9):1039-1050.
[21]	EYBL V,KOTYZOVA D,KOUTENSKY J.Comparative study of natural antioxidants-curcumin,resveratrol and melatonin-in cadmium-induced oxidative damage in mice[J].Toxicology,2006,225(2-3):150-156.
[22]	LI R Y,LUO X,LI L B,et al.The protective effects of melatonin against oxidative stress and inflammation induced by acute cadmium exposure in mice testis[J].Biol Trace Elem Res,2016,170(1):152-164.

褪黑素对镉致鸭大脑皮质毒性损伤的保护作用

Protective Effect of Melatonin on Toxic Damage Caused by Cadmium in Cerebral Cortex of Duck

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 22

相关文章 15

编辑推荐

Metrics

本文评价

[1]	林燕, 黄敏, 李秀金, 张续勐, 黄运茂, 田允波, 伍仲平. 利用全基因组重测序数据检测8个鸭品种基因组拷贝数变异[J]. 畜牧兽医学报, 2023, 54(9): 3700-3709.
[2]	李倩倩, 林馨, 韦依琳, 崔昊宇, 邹荣华, 吴晓妮, 葛家振, 黄国梁, 张丽娟, 郑福英, 蔺国珍. RaeR对鸭疫里默氏杆菌外排泵RaeC-RaeA-RaeB的调控作用研究[J]. 畜牧兽医学报, 2023, 54(9): 3793-3802.
[3]	贺名扬, 马钰静, 王泳, 杨若晨, 刘月琴, 张英杰, 段春辉. 褪黑激素对绵羊卵巢颗粒细胞增殖、凋亡、类固醇激素分泌的影响[J]. 畜牧兽医学报, 2023, 54(8): 3313-3324.
[4]	曾成容, 王娜, 毕文文, 梅世慧, 何广霞, 张峻杰, 陈泽, 文明, 周碧君. A型产气荚膜梭菌感染鸭回肠代谢组学分析[J]. 畜牧兽医学报, 2023, 54(6): 2555-2569.
[5]	赵栋皓, 原梦, 马凯腾, 段卓, 祝一鑫, 唐芳, 韩克光, 霍乃蕊. 羊骨胶原肽对镉的螯合作用及对镉致鸡肝损伤的干预作用[J]. 畜牧兽医学报, 2023, 54(6): 2641-2652.
[6]	孙俊峰, 潘建秋, 张焯深, 江丹莉, 沈栩, 陈蓉, 许丹宁, 田允波, 黄运茂. OPN5-TSH-DIO2/DIO3和VIP-PRL在短光照抑制公山麻鸭睾丸活性中的表达规律[J]. 畜牧兽医学报, 2023, 54(5): 2001-2012.
[7]	曹秀芸, 刘吉文, 汤智辉, 郑紫怡, 闫丽萍, 宋素泉. 一株鸭腺病毒3型的分离、鉴定及致病性分析[J]. 畜牧兽医学报, 2023, 54(5): 2050-2061.
[8]	常颖艳, 赵鸿雁, 胡茂志, 刘宗平. 枸杞酰胺体外对镉致成骨细胞凋亡的保护作用[J]. 畜牧兽医学报, 2023, 54(3): 1273-1280.
[9]	陈敬宜, 于淼, 张金洋, 樊堃, 杨桂君, 葛铭, 张瑞莉. 铁死亡参与镉暴露鸡肝损伤的研究[J]. 畜牧兽医学报, 2023, 54(2): 787-802.
[10]	李春艳, 张彦, 吕春荣, 邓卫东, 权国波. 褪黑素的抗氧化机制及其在哺乳动物精子冷冻保存中的应用研究进展[J]. 畜牧兽医学报, 2023, 54(11): 4468-4476.
[11]	张倩, 崔燕, 余四九, 何俊峰, 潘阳阳, 王萌. 初生及成年牦牛大脑皮质的转录组分析[J]. 畜牧兽医学报, 2023, 54(11): 4605-4614.
[12]	郭艳红, 唐静, 张博, 曹俊婷, 郭占宝, 谢明, 周正奎, 吴永保, 闻治国. 饲粮中代谢能和蛋氨酸水平对育肥期北京鸭生长性能、屠宰性能和血浆生化指标的影响[J]. 畜牧兽医学报, 2023, 54(10): 4278-4288.
[13]	仝锡帅, 余耿生, 李雅文, 冉迪, 顾建红, 邹辉, 袁燕, 卞建春, 刘宗平. NAC通过抑制大鼠肺细胞凋亡缓解镉致肺组织损伤[J]. 畜牧兽医学报, 2022, 53(9): 3241-3250.
[14]	杨伏春, 刘芮, 李晓涵, 高立, 刘长军, 祁小乐, 崔红玉, 王笑梅, 高玉龙, 李凯. 鸭肠炎病毒UL41基因缺失株的构建及其体外增殖能力分析[J]. 畜牧兽医学报, 2022, 53(8): 2689-2696.
[15]	张羽晨, 雷亚萍, 农碧丹, 庞晓敏, 张苗苗, 刘晓丽, 谷长勤, 张万坡, 程国富, 胡薛英. 自然感染鸭坦布苏病毒蛋鸭卵巢的病理学观察[J]. 畜牧兽医学报, 2022, 53(7): 2326-2332.