手掌参多糖提取物对氧化应激舍饲肉羊生产性能、抗氧化机能及肉品质的影响

doi:10.11843/j.issn.0366-6964.2020.09.016

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (9): 2187-2196.doi: 10.11843/j.issn.0366-6964.2020.09.016

手掌参多糖提取物对氧化应激舍饲肉羊生产性能、抗氧化机能及肉品质的影响

萨茹丽¹, 杨斌^2*, 格根哈斯³, 钱琳娜², 赵世华^2*

1. 内蒙古农业大学动物科学学院, 内蒙古自治区高校动物营养与饲料科学重点实验室, 呼和浩特 010018;
2. 内蒙古自治区农牧业科学院兽医研究所, 呼和浩特 010031;
3. 内蒙古自治区锡林郭勒盟正蓝旗旗委办公室, 锡林郭勒盟 027200

收稿日期:2020-02-17 出版日期:2020-09-25 发布日期:2020-09-25
通讯作者: 杨斌,主要从事家畜营养代谢病及应激性疾病防控研究,E-mail:yangbin@imaaahs.ac.cn;赵世华,主要从事家畜营养代谢病防控研究,E-mail:zhaoshihua8@163.com
作者简介:萨茹丽(1986-),女,蒙古族,内蒙古自治区兴安盟人,讲师,博士,主要从事反刍动物营养研究,E-mail:qisaruli@126.com
基金资助:
国家肉羊产业技术体系（CARS-38）；内蒙古农牧业创新基金项目（2019CXJJM10）

Effects of Gymnadenia Conopsea Polysaccharide Extract on Growth Performance, Antioxidant Function and Meat Quality of House-fed Mutton Sheep under Oxidative Stress

SA Ruli¹, YANG Bin^2*, GE Genhasi³, QIAN Linna², ZHAO Shihua^2*

1. Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Institute of Veterinary Medicine, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China;
3. Chinese Communist Party ZhengLan Banner Committee, Xilingol 027200, China

Received:2020-02-17 Online:2020-09-25 Published:2020-09-25

摘要/Abstract

摘要： 本试验旨在研究手掌参多糖提取物对氧化应激舍饲肉羊生长性能、肉品质及抗氧化应激作用的影响，为手掌参及其提取物的深入研究与开发提供依据。试验选用20只6月龄体重为（35.0±4.0）kg的健康雄性小尾寒羊，随机分为两组，每组10只羊，分别为试验组和对照组。整个试验期42 d，一共分为3个阶段，前15 d为空白期，中间15 d为处理期，后12 d为造模后观测期。处理期第1天开始，对照组饲喂基础日粮，试验组饲喂在基础日粮内添加30 mg·kg^-1手掌参粗多糖提取物的颗粒饲料。在处理期第15天时，对各组试验羊只按体重（BW）一次性腹腔注射Diquat（敌草快）溶液10 mg·kg^-1，制造氧化应激模型，进入造模后观测期。在整个试验期第1、15、30、33、36、39和42天晨饲前空腹称重并采血，分离血清，测定血清中的抗氧化指标（SOD、GSH-Px、T-AOC、MDA）。在试验期第42天，每组随机选取5只羊屠宰后采集肉样，检测肉品质相关指标。试验结果显示，舍饲肉羊日粮中添加手掌参多糖提取物可显著提高肉羊平均日采食量（P<0.05），且当机体遭受氧化应激时，日粮中添加手掌参多糖提取物的试验处理组平均日采食量、平均日增重以及抗氧化酶含量等指标恢复较快，羊肉滴水损失显著降低，羊肉剪切力显著提高（P<0.05）。试验结果证明，手掌参多糖提取物可提高肉羊生产性能，且能缓解氧化应激造成的生产性能和抗氧化性能下降的问题，具有一定的抗氧化应激作用。

关键词: 手掌参, 氧化应激, 生产性能, 肉品质

Abstract: The purpose of this experiment was to study the effects of Gymnadenia conopsea polysaccharide (GCP) extract on growth performance, meat quality and antioxidant function house-fed mutton sheep under oxidative stress, so as to provide experimental basis for extensive research and development of Gymnadenia conopsea and its extracts. Twenty 6-month-old healthy male Small-Tailed Han sheep with body weight of (35.0 ±4.0) kg were randomly divided into two groups. The experimental group (n=10) and control group(n=10). The whole experiment lasted for 42 d and was divided into 3 periods: the first 15 days were the blank period; the middle 15 days were the treatment period; the last 12 days were the observation period ofter modeling. From the first day of the treatment period, the control group was fed with basal diet, and the experimental group was fed pellet basal diet containing 30 mg·kg^-1 GCP extract. On the 15th day of the treatment period, the sheep in each group were intraperitoneally injected with 10 mg·kg^-1 Diquat solution according to sheep body weight(BW), and then fed for another 12 days. On the 1 st, 15th, 30th, 33rd, 36th, 39th and 42nd morning of the whole experimental period, fasting weight and blood samples were collected before feeding. The serum was separated and the antioxidant indexes (SOD, GSH-Px, T-AOC, MDA) in serum were determined. On the 42nd day of the whole experimental period, 5 sheep randomly selected from each group were slaughtered and meat samples were collected to determine the meat quality. The results showed that the addition of GCP extract to the diet of the sheep could increase the average daily feed intake (P<0.05). When the body was subjected to oxidative stress, the indexes of daily gain and antioxidant enzymes recovered rapidly on experimental group, the mutton drip loss decreased significantly, and the mutton sheer force increased significantly(P<0.05). The results indicated that GCP extract could improve the growth performance of mutton sheep, and alleviated the decline of growth performance and antioxidant performance caused by oxidative stress, and had a certain effect of antioxidant stress.

Key words: Gymnadenia conopsea, oxidative stress, growth performance, meat quality

中图分类号:

S826.92.1.5

萨茹丽, 杨斌, 格根哈斯, 钱琳娜, 赵世华. 手掌参多糖提取物对氧化应激舍饲肉羊生产性能、抗氧化机能及肉品质的影响[J]. 畜牧兽医学报, 2020, 51(9): 2187-2196.

SA Ruli, YANG Bin, GE Genhasi, QIAN Linna, ZHAO Shihua. Effects of Gymnadenia Conopsea Polysaccharide Extract on Growth Performance, Antioxidant Function and Meat Quality of House-fed Mutton Sheep under Oxidative Stress[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2187-2196.

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手掌参多糖提取物对氧化应激舍饲肉羊生产性能、抗氧化机能及肉品质的影响

Effects of Gymnadenia Conopsea Polysaccharide Extract on Growth Performance, Antioxidant Function and Meat Quality of House-fed Mutton Sheep under Oxidative Stress

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