马齿苋水提物对热应激小鼠空肠结构及吸收功能的影响

doi:10.11843/j.issn.0366-6964.2020.04.024

摘要/Abstract

摘要： 旨在探讨马齿苋水提物（aqueous extract of Portulaca oleracea L.）对热应激小鼠空肠结构及吸收功能的影响。将40只昆明种小鼠随机分为4组（n=10）：空白对照组（C）、热应激组（HS）、马齿苋水提物组（AEP）、维生素C组（Vc），HS、AEP和Vc组每天于（40±1）℃环境下处理0.5 h，连续热应激6 d后将小鼠转置于室温下给药治疗。给药7 d后眶窦采血，并采集小鼠空肠；测定小鼠血清中D-木糖、葡萄糖含量；HE染色观察空肠组织病理学变化；qRT-PCR法检测空肠黏膜ZO-1、SGLT1及GLUT2 mRNA的相对转录水平。结果显示，与C组比较，HS组小鼠空肠黏膜绒毛高度极显著降低（P<0.01）；血清中D-木糖的含量极显著降低（P<0.01）；空肠黏膜中ZO-1、SGLT1和GLUT2 mRNA的相对转录水平均极显著降低（P<0.01）；与HS组相比，AEP组小鼠空肠绒毛高度极显著提高（P<0.01）；血清中D-木糖及葡萄糖的含量均显著升高（P<0.01或P<0.05）；空肠黏膜中ZO-1、SGLT1和GLUT2 mRNA的相对转录水平均显著升高（P<0.05或P<0.01）。高温处理可导致小鼠空肠黏膜脱落及吸收功能下降，而在给予马齿苋水提物治疗后可通过提高空肠ZO-1 mRNA表达量修复空肠黏膜结构，通过促进空肠黏膜SGLT1和GLUT2 mRNA的转录量改善吸收功能。马齿苋水提物能在一定程度上修复热应激导致小鼠空肠黏膜结构损伤，改善热应激小鼠肠道的吸收能力。

关键词: 热应激, 小鼠, 马齿苋水提物, 空肠结构, 空肠吸收

Abstract: The present study was conducted to investigate the effects of aqueous extract of Portulaca oleracea L. (AEP) on the structure and function of jejunum in mouse under the heat stress conditions. A total of 40 Kunming mice were randomly allocated to 4 groups:control group (C), heat stress group (HS), aqueous extract of Portulaca oleracea L. (AEP), and vitamin C group (Vc), heat stress (in groupHS, AEP &Vc) was conducted by exposing mice to (40±1)℃ for 0.5 h, and after 6 days of continuous heat stress, the mice were transferred to room temperature for treatment. Seven days after the administration, blood samples were collected from the orbital sinus and the jejunum of the mice was collected. The contents of D-xylose and glucose in the serum of mice were measured; the pathological changes of jejunum were observed by HE staining; qRT-PCR was used to detect the relative transcription levels of ZO-1, SGLT1 and GLUT2 mRNA in the jejunal mucosa. The results showed that compared with group C, the jejunal mucosal villi height of the HS group mice was significantly reduced (P<0.01); the level of D-xylose in the serum were significantly reduced (P<0.01); the relative transcription levels of ZO-1, SGLT1 and GLUT2 mRNA in the jejunal mucosa were significantly reduced (P<0.01); compared with the HS group mice, the jejunum villi of the AEP group mice were significantly increased (P<0.01); the levels of D-xylose and glucose in serum were significantly increased (P <0.01 or P<0.05); the relative transcription levels of ZO-1, SGLT1 and GLUT2 mRNA in the jejunal mucosa were significantly increased (P<0.05 or P<0.01). High temperature treatment can induce the jejunum mucosa to fall off and decrease the absorption function. After giving purslane extract treatment, the jejunum mucosa structure can be repaired by increasing the jejunum ZO-1 mRNA transcription, and absorption functioncan be improved by promoting the jejunum mucosa SGLT1 and GLUT2 mRNA transcription. AEP can repair the jejunum mucosa damage caused by heat stress to a certain level, and improve the intestinal absorption capacity of heat stress mice.

Key words: heat stress, mouse, aqueous extract of Portulaca oleracea L., jejunal structure, jejunal absorption

中图分类号:

S853.7

郭月, 刘小宝, 李健, 何欣, 赵兴华. 马齿苋水提物对热应激小鼠空肠结构及吸收功能的影响[J]. 畜牧兽医学报, 2020, 51(4): 873-880.

GUO Yue, LIU Xiaobao, LI Jian, HE Xin, ZHAO Xinghua. The Effects of Aqueous Extract of Portulaca oleracea L.on the Structure and Function of Jejunum in Mice with Heat Stress[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 873-880.

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