引起犊牛腹泻的Nebovirus

doi:10.11843/j.issn.0366-6964.2020.03.004

摘要/Abstract

摘要： Newbury-1 virus目前是杯状病毒科（Caliciviridae）纽布病毒属（Nebovirus）的唯一成员，常用Nebovirus（NeV）表述。NeV为单股正链RNA病毒，基因组全长为7 453~7 460 bp，是致犊牛腹泻的重要病毒。NeV的病原检测主要依靠RT-PCR和Real-time RT-PCR方法。迄今为止，NeV已经在美国、英国、巴西、土耳其等13个国家检出，具有广泛的地域分布。最近，本实验室的研究表明该病毒在我国奶牛和牦牛中广泛流行，具有独特的进化趋势，并且有新基因型毒株的出现。本文就NeV的生物学特性、流行概况、所致疾病的临床症状与病理变化、检测方法和防控措施等研究进展进行综述，以期为NeV的研究提供参考。

关键词: nebovirus, 犊牛腹泻, 病原学, 流行病学, 检测

Abstract: Newbury-1 virus, usually designated by Nebovirus (NeV), is the only member of the genus Nebovirus in the family Caliciviridae at present. NeV is a single-stranded positive-sense RNA virus with a genome length of 7 453-7 460 bp and is an important diarrhea-causing virus in calves. The detection of NeV mainly relies on RT-PCR and Real-time RT-PCR assays. To date, NeV has been detected in 13 countries including the USA, the UK, Brazil, Turkey, etc., suggesting that NeV has been widely distributed around the world. Recently, our studies suggested that the virus has been widely circulating among dairy cows and yak in China, presenting a unique evolution trend, and new genotypes have been determined. To provide a reference for NeV research, this review describes the latest research progress on NeV, including the biological characteristics, epidemiology, clinical symptoms and pathology, detection methods, prevention and control.

Key words: Nebovirus, calves diarrhea, etiology, epidemiology, detection

中图分类号:

S852.659.6

郭紫晶, 何琪富, 岳华, 汤承. 引起犊牛腹泻的Nebovirus[J]. 畜牧兽医学报, 2020, 51(3): 433-442.

GUO Zijing, HE Qifu, YUE Hua, TANG Cheng. Nebovirus: a Diarrhea-causing Virus in Calves[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 433-442.

参考文献 53

[1]	CHO E H, SOLIMAN M, ALFAJARO M M, et al. Bovine nebovirus interacts with a wide spectrum of histo-blood group antigens[J]. J Virol, 2018, 92(9):e02160-17.
[2]	KRONEMAN A, VEGA E, VENNEMA H, et al. Proposal for a unified norovirus nomenclature and genotyping[J]. Arch Virol, 2013, 159(10):2059-2068.
[3]	LE PENDU J, ABRANTES J, BERTAGNOLI S, et al. Proposal for a unified classification system and nomenclature of lagoviruses[J]. J Gen Virol, 2017, 98(7):1658-1666.
[4]	GUO Z J, HE Q F, ZHANG B, et al. First detection of neboviruses in Yak (Bos grunniens) and identification of a novel neboviruses based on complete genome[J]. Vet Microbiol, 2019, 236:108388, doi:10. 1016/j. vetmic. 2019. 108388.
[5]	D'MELLO F, JERVIS S M, EDWARDS P M, et al. Heterogeneity in the capsid protein of bovine enteric caliciviruses belonging to a new genus[J]. Virology, 2009, 387(1):109-116.
[6]	SMILEY J R, CHANG K O, HAYES J, et al. Characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus[J]. J Virol, 2002, 76(20):10089-10098.
[7]	BRIDGER J C, HALL G A, BROWN J F. Characterization of a calici-like virus (Newbury agent) found in association with astrovirus in bovine diarrhea[J]. Infect Immun, 1984, 43(1):133-138.
[8]	GUO Z J, HE Q F, YUE H, et al. First detection of nebovirus and norovirus from cattle in China[J]. Arch Virol, 2018, 163(2):475-478.
[9]	GUO Z J, HE Q F, ZHANG B, et al. Detection and molecular characteristics of neboviruses in dairy cows in China[J]. J Gen Virol, 2019, 100(1):35-45.
[10]	GUO Z J, HE Q F, YUE H, et al. Genomic characterization of a RdRp-recombinat nebovirus strain with a novel VP1 genotype[J]. Virus Res, 2018, 251:6-13.
[11]	CANDIDO M, ALENCAR A L F, ALMEIDA-QUEIROZ S R, et al. First detection and molecular characterization of Nebovirus in Brazil[J]. Epidemiol Infect, 2016, 144(9):1876-1878.
[12]	DI MARTINO B, DI PROFIO F, MARTELLA V, et al. Evidence for recombination in neboviruses[J]. Vet Microbiol, 2011, 153(3-4):367-372.
[13]	HASSINE-ZAAFRANE M, KAPLON J, SDIRI-LOULIZI K, et al. Molecular prevalence of bovine noroviruses and neboviruses detected in central-eastern Tunisia[J]. Arch Virol, 2012, 157(8):1599-1604.
[14]	KAPLON J, GUENAU E, ASDRUBAL P, et al. Possible novel nebovirus genotype in cattle, France[J]. Emerg Infect Dis, 2011, 17(6):1120-1123.
[15]	KARAYEL-HACIOGLU I, ALKAN F. Molecular characterization of bovine noroviruses and neboviruses in Turkey:detection of recombinant strains[J]. Arch Virol, 2019, 164(5):1411-1417.
[16]	TURAN T, IŞIDAN H, ATASOY M O, et al. Detection and molecular analysis of bovine enteric norovirus and nebovirus in Turkey[J]. J Vet Res, 2018, 62(2):129-135.
[17]	CHO Y I, HAN J I, WANG C, et al. Case-control study of microbiological etiology associated with calf diarrhea[J]. Vet Microbiol, 2013, 166(3-4):375-385.
[18]	PARK S I, JEONG C, PARK S J, et al. Molecular detection and characterization of unclassified bovine enteric caliciviruses in South Korea[J]. Vet Microbiol, 2008, 130(3-4):371-379.
[19]	POURASGARI F, KAPLON J, SANCHOOLI A, et al. Molecular prevalence of bovine noroviruses and neboviruses in newborn calves in Iran[J]. Arch Virol, 2018, 163(5):1271-1277.
[20]	REUTER G, KÁTAI A, KÁLMÁN M, et al. First detection of human calicivirus infection in Hungary[J]. Orv Hetil, 2000, 141(38):2071-2074.
[21]	DESSELBERGER U. Caliciviridae other than noroviruses[J]. Viruses, 2019, 11(3):E286.
[22]	OLIVER S L, ASOBAYIRE E, DASTJERDI A M, et al. Genomic characterization of the unclassified bovine enteric virus Newbury agent-1(Newbury1) endorses a new genus in the family Caliciviridae[J]. Virology, 2006, 350(1):240-250.
[23]	SMERTINA E, URAKOVA N, STRIVE T, et al. Calicivirus RNA-Dependent RNA Polymerases:evolution, structure, protein dynamics, and function[J]. Front Microbiol, 2019, 10:1280.
[24]	DEVAL J, JIN Z N, CHUANG Y C, et al. Structure(s), function(s), and inhibition of the RNA-dependent RNA polymerase of noroviruses[J]. Virus Res, 2017, 234:21-33.
[25]	LEE J H, CHUNG M S, KIMM K H. Structure and function of Caliciviral RNA polymerases[J]. Viruses, 2017, 9(11):E329.
[26]	OKA T, WANG Q H, KATAYAMA K, et al. Comprehensive review of human sapoviruses[J]. Clin Microbiol Rev, 2015, 28(1):32-53.
[27]	DI FELICE E, MAUROY A, DAL POZZO F, et al. Bovine noroviruses:a missing component of calf diarrhoea diagnosis[J]. Vet J, 2016, 207:53-62.
[28]	BULL R A, EDEN J S, RAWLINSON W D, et al. Rapid evolution of pandemic noroviruses of the GII. 4 lineage[J]. PLoS Pathog, 2010, 6(3):e1000831.
[29]	SMITH H Q, SMITH T J. The dynamic capsid structures of the Noroviruses[J]. Viruses, 2019, 11(3):235.
[30]	HANSMAN G S, NATORI K, SHIRATO-HORIKOSHI H, et al. Genetic and antigenic diversity among noroviruses[J]. J Gen Virol, 2006, 87(4):909-919.
[31]	ZHANG G B, WANG J, LIU J J, et al. The surface-exposed loop region of norovirus GII. 3 VP1 plays an essential role in binding histo-blood group antigens[J]. Arch Virol, 2019, 164(6):1629-1638.
[32]	ALKAN F, KARAYEL İ, CATELLA C, et al. Identification of a bovine enteric calicivirus, klrklareli virus, distantly related to Neboviruses, in calves with enteritis in Turkey[J]. J Clin Microbiol, 2015, 53(11):3614-3617.
[33]	MATHIJS E, DE OLIVEIRA-FILHO E F, DAL POZZO F, et al. Infectivity of a recombinant murine norovirus (RecMNV) in Balb/cByJ mice[J]. Vet Microbiol, 2016, 192:118-122.
[34]	LUDWIG-BEGALL L F, MAUROY A, THIRY E. Norovirus recombinants:recurrent in the field, recalcitrant in the lab-a scoping review of recombination and recombinant types of noroviruses[J]. J Gen Virol, 2018, 99(8):970-988.
[35]	SILVÉRIO D, LOPES A M, MELO-FERREIRA J, et al. Insights into the evolution of the new variant rabbit haemorrhagic disease virus (GI. 2) and the identification of novel recombinant strains[J]. Transbound Emerg Dis, 2018, 65(4):983-992.
[36]	KHAMRIN P, KUMTHIP K, SUPADEJ K, et al. Noroviruses and sapoviruses associated with acute gastroenteritis in pediatric patients in Thailand:increased detection of recombinant norovirus GII. P16/GII. 13 strains[J]. Arch Virol, 2017, 162(11):3371-3380.
[37]	WHITE P A. Evolution of norovirus[J]. Clin Microbiol Infect, 2014,20(8):741-745.
[38]	EDEN J S, TANAKA M M, BONI M F, et al. Recombination within the pandemic norovirus GII. 4 lineage[J]. J Virol, 2013, 87(11):6270-6282.
[39]	LUCERO Y, VIDAL R, O'RYAN G M. Norovirus vaccines under development[J]. Vaccine, 2018, 36(36):5435-5441.
[40]	YANG B, YANG B, SHAN X N, et al. Short communication:Immune responses in sows induced by porcine sapovirus virus-like particles reduce viral shedding in suckled piglets[J]. Res Vet Sci, 2018, 117:196-199.
[41]	THOMAS C, JUNG K, HAN M G, et al. Retrospective serosurveillance of bovine norovirus (GIII. 2) and nebovirus in cattle from selected feedlots and a veal calf farm in 1999 to 2001 in the United States[J]. Arch Virol, 2014, 159(1):83-90.
[42]	LU Z Y, YOKOYAMA M, CHEN N, et al. Mechanism of cell culture adaptation of an enteric calicivirus, the porcine sapovirus Cowden Strain[J]. J Virol, 2015, 90(3):1345-1358.
[43]	WOBUS C E, KARST S M, THACKRAY L B, et al. Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages[J]. PLoS Biol, 2004, 2(12):e432.
[44]	ESTES M K, ETTAYEBI K, TENGE V R, et al. Human norovirus cultivation in nontransformed stem cell-derived human intestinal enteroid cultures:success and challenges[J]. Viruses, 2019, 11(7):638.
[45]	TAKANASHI S, SAIF L J, HUGHES J H, et al. Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures[J]. Arch Virol, 2014, 159(2):257-266.
[46]	JONES M K, WATANABE M, ZHU S, et al. Enteric bacteria promote human and mouse norovirus infection of B cells[J]. Science, 2014, 346(6210):755-759.
[47]	ETTAYEBI K, CRAWFORD S E, MURAKAMI K, et al. Replication of human noroviruses in stem cell-derived human enteroids[J]. Science, 2016, 353(6306):1387-1393.
[48]	JONES M K, GRAU K R, COSTANTINI V, et al. Human norovirus culture in B cells[J]. Nat Protoc, 2015, 10(12):1939-1947.
[49]	KILIC T, KOROMYSLOVA A, HANSMAN G S. Structural basis for human norovirus capsid binding to bile acids[J]. J Virol, 2019, 93(2):e01581-18.
[50]	PARK S I, PARK D H, SAIF L J, et al. Development of SYBR Green real-time RT-PCR for rapid detection, quantitation and diagnosis of unclassified bovine enteric calicivirus[J]. J Virol Methods, 2009, 159(1):64-68.
[51]	SMILEY J R, HOET A E, TRÅVÉN M, et al. Reverse Transcription-PCR Assays for Detection of Bovine Enteric Caliciviruses (BEC) and analysis of the genetic relationships among BEC and human Caliciviruses[J]. J Clin Microbiol, 2003, 41(7):3089-3099.
[52]	PARK S J, JEONG C, YOON S S, et al. Detection and characterization of bovine coronaviruses in fecal specimens of adult cattle with diarrhea during the warmer seasons[J]. J Clin Microbiol, 2006, 44(9):3178-3188.
[53]	郭紫晶, 何琪富, 汤承, 等. 检测纽布病毒的Real-time RT-PCR方法的建立与应用[J]. 畜牧兽医学报, 2019, 50(4):893-900.GUO Z J, HE Q F, TANG C, et al. Establishment and application of a Real-time RT-PCR assay for detecting bovine nebovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(4):893-900. (in Chinese)