钻孔法增加犬半月板脱细胞基质支架孔隙率的可行性分析

doi:10.11843/j.issn.0366-6964.2020.01.020

摘要/Abstract

摘要： 为增加半月板脱细胞基质（MAM）支架的孔隙率，提高细胞植入量，笔者对犬半月板先行钻孔，再行脱细胞处理，然后检测支架的脱细胞效果、孔隙率和力学性能变化、细胞植入量及与犬骨髓间充质干细胞（BMSCs）向软骨诱导培养后糖胺聚糖和Ⅱ型胶原蛋白的表达情况。结果显示，钻孔支架的脱细胞效果良好，未见细胞核，胶原纤维结构完整；支架的孔隙率显著增大，但压缩模量变化不显著；BMSCs在钻孔支架中的植入量显著增加，细胞增殖速度和向软骨分化能力显著提高。结果表明，钻孔法增加半月板基质支架的孔隙率，不但能保持支架的力学性能、提高细胞的植入量，而且能促进细胞的增殖、分化和组织重塑，为MAM支架在组织工程半月板构建中的广泛应用奠定了基础。

关键词: 钻孔法, 半月板脱细胞基质, 孔隙率, 犬

Abstract: In order to increase the porosity of the meniscus acellular matrix (MAM) scaffolds and improve the amount of cells implanted in them, canine menisci were drilled firstly and then decellularized in this experiment. Next, the decellularization effect and changes in porosity and mechanical properties of the scaffolds were detected. The amount of cells implanted and expression of glycosaminoglycan and type Ⅱ collagen in the scaffolds were examined after chondrogenic induction with BMSCs (bone marrow mesenchymal stem cells). The results showed that the decellularization effect of the drilled scaffold was good, no nuclei were found, and the collagen fibers were structurally intact. The porosity of the scaffold was significantly increased, but the compressive modulus did not change significantly. The amount of BMSCs implanted in the drill scaffold was significantly increased. The rate of cell proliferation and the ability to differentiate into cartilage were significantly improved. The results indicated that the drilling method used can increase the porosity of the meniscus matrix scaffold, which not only maintains the mechanical properties of the scaffold and increases the amount of cells implanted, but also promotes cell proliferation, differentiation and tissue remodeling. It laid a foundation for the wide application of MAM scaffolds in tissue engineering meniscus construction.

Key words: drilling method, meniscus acellular matrix, porosity, canine

中图分类号:

S857.16

邹彤, 赵雯, 吕阳欧, 高登科, 阮晨梅, 张霞, 张翊华. 钻孔法增加犬半月板脱细胞基质支架孔隙率的可行性分析[J]. 畜牧兽医学报, 2020, 51(1): 179-186.

ZOU Tong, ZHAO Wen, Lü Yang'ou, GAO Dengke, RUAN Chenmei, ZHANG Xia, ZHANG Yihua. Feasibility Analysis of Drilling Method to Increase Porosity of Canine Meniscus Acellular Matrix Scaffolds[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 179-186.

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