猪圆环病毒2型合并感染脑心肌炎病毒对仔猪的致病性分析

doi:10.11843/j.issn.0366-6964.2016.07.017

Abstract

Abstract:

The objective of our research was to evaluate the growth performance and pathogenicity of piglets concurrent infection with porcine encepholomyocarditis virus (EMCV) under immunosuppression condition caused by porcine circovirus type 2 (PCV-2).Sixteen piglets were chosen for three challenge groups including EMCV，PCV-2 and concurrent infection with PCV-2 and EMCV，and control group.The effect of concurrent infection on piglets was conducted by the relevant indexes including clinical symptoms，pathological examinations，growth performances，immune index，detoxification and so on.The results indicated that the groups infection only with EMCV or PCV-2 showed their own classical clinical characteristic and minimal injury.However，the mortality rate of concurrent infection increased rapidly，the average daily weight gain decreased significantly，degeneration and necrosis in myocardium and brain tissues was more severe，the lymphocyte ratio in the blood still remained low and the neutraliz-ation antibody of EMCV maintained at a low level in a long time.The piglets shed virus lasted 12 days after infection，longer than the group infected only with EMCV for 9 days.The results showed that PCV-2 early infection can promote more severe pathogenicity and lethality，and lower immunity in piglets induced by EMCV，and the ability of virus replication and detoxification in piglets enhanced.

Key words: PCV-2, EMCV, concurrent infection, piglets, pathogenicity

CLC Number:

S858.285.3

CHANG Hong-tao，LIU Hui-min，LI Yong-tao，HE Xiu-yuan，ZHAO Jun，CHEN Lu，WANG Xin-wei，YANG Xia，WANG Chuan-qing. Analysis of Pathogenicity in Piglets Concurrent Infection with Encepholomyocarditis Virus under Immunosuppression Caused by Porcine Circovirus Type 2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1435-1442.

References

［1］施开创，陈宏备，覃芳芸，等.猪脑心肌炎病毒TaqMan荧光定量RT-PCR检测方法的建立及应用［J］.中国兽医科学，2011，41（9）：928-932.
SHI K C，CHEN H B，QIN F Y，et al.Development and application of a TaqMan real-time RT-PCR assay for the detection of porcine encephalomyocarditis virus［J］．Chinese Veterinary Science，2011，41（9）：928-932.（in Chinese）
［2］盖新娜，杨汉春，郭鑫，等．猪脑心肌炎病毒的分离与鉴定［J］．畜牧兽医学报，2007，38（1）：59-65．
GE X N，YANG H C，GUO X，et al.Isolation and characterization of porcine encephalomyocarditis virus［J］．Acta Veterinaria et Zootechnica Sinica，2007，38（1）：59-65．（in Chinese）
［3］YUAN W Z，SONG Q Y，ZHANG X Y，et al.Isolation and molecular analysis of porcine encephalomyocarditis virus strain BD２ from northern China［J］.Infect Genet Evol，2014，21：303-307.
［4］LIU H M，YAN Q，ZHAO B，et al.Isolation，molecular characterization，and phylogenetic analysis of encephalomyocarditis virus from South China tigers in China［J］．Infect Genet Evol，2013，19：240-243．
［5］常洪涛，刘慧敏，陈陆，等．不同动物源脑心肌炎病毒的分离、鉴定和全基因组序列分析［J］．中国兽医学报，2014，34（9）：1442-1447．
CHANG H T，LIU H M，CHEN L，et al.Isolation，identification and full length genome sequence analysis of EMCV from different animal source［J］．Chinese Journal of Veterinary Science，2014，34（9）：1442-1447．（in Chinese）
［6］陆文俊，施开创，屈素洁，等.猪脑心肌炎病毒抗体间接ELISA检测方法的建立及应用［J］.动物医学进展，2011，32（12）：6-10.
LU W J，SHI K C，QU S J，et al.Development and application of an indirect ELISA for detection of antibody against porcine encephalomyocarditis virus［J］. Progress in Veterinary Medicine，2011，32（12）：6-10.（in Chinese）
［7］樊得英，冯若飞，李向茸，等.脑心肌炎病毒抗体双抗原夹心ELISA检测方法的建立与初步应用［J］.中国生物制品学杂志，2012，25（1）：104-107.
FAN D Y，FENG R F，LI X R，et al.Development and preliminary application of double antigen sandwich ELISA method for encephalomyocarditis virus［J］.Chinese Journal of Biologicals，2012，25（1）：104-107.（in Chinese）
［8］冯若飞.脑心肌炎病毒双抗原夹心ELISA建立、血清学调查、毒株分离及RNA干扰研究［D］.北京：中国农业科学院，2014.
FENG R F.DS-ELISA established，serosurvey，strains isolation and RNAi for EMCV［D］.Beijing：Chinese Academy of Agricultural Sciences，2014.（in Chinese）
［9］张家龙，盖新娜，马良，等.规模化猪场脑心肌炎病毒感染的血清学调查［J］.中国兽医杂志，2007，43（1）：7-9.
ZHANG J L，GE X N，MA L，et al.Serological survey of EMCV infection in pigs on large-scale pig farms in China 2005-2006［J］. Chinese Journal of Veterinary Medicine，2007，43（1）：7-9.（in Chinese）
［10］PALMENBERG A C，KIRDY E M，JANDA M R，et al.The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region［J］.Nucleic Acids Res，1984，12(6)：2969-2985.
［11］SEGALÉS J，DOMINGO M，CHIANINI F，et al.Immunosuppression in postweaning multisystemic wasting syndrome affected pigs［J］.Vet Microbiol，2004，98(2)：151-158.
［12］刘捷，陆琪，王先炜，等.猪圆环病毒2型、猪巨细胞病毒和TTV-2混合感染的流行病学调查［J］.中国兽医学报，2011，31（8）：1095-1098.
LIU J，LU Q，WANG X W，et al.Epidemiological investigation of porcine circovirus type2，porcine cytomegalovirus and Torque Teno virus 2 infection［J］.Chinese Journal of Veterinary Science，2011，31（8）：1095-1098.（in Chinese）
［13］MCNEILLY F，KENNEDY S，MOFFETT D，et al.A comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus formalin-fixed tissues from pigs with post-weaning multisystemic wasting syndrome（PMWS）［J］.J Virol Methods，1999，80（2）：123-128.
［14］施开创，杨汉春，郭鑫，等.猪繁殖与呼吸综合征病毒和猪圆环病毒2型共感染对猪体内病毒动态及免疫反应的影响［J］.畜牧兽医学报，2009，40（2）：228-234.
SHI K C，YANG H C，GUO X，et al.Effects of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 co-infection on virological kinetics and immunological responses in piglets［J］. Acta Veterinaria et Zootechnica Sinica，2009，40（2）：228-234.（in Chinese）
［15］KIM J，HA Y，CHAE C.Potentiation of porcine circovirus 2-induced postweaning multisystemic wasting syndrome by porcine parvovirus is associated with excessive production of tumor necrosis factor-α［J］.Vet Pathol，2006，43（5）：718-725.
［16］刘祥义，陈立功，宋勤叶，等.猪圆环病毒2型与猪细小病毒共感染对猪肺泡巨噬细胞吞噬功能及其干扰素表达水平的影响［J］.微生物学报，2011，51（1）：105-114.
LIU X Y，CHEN L G，SONG Q Y，et al.Coinfection effects of porcine circovirus type 2 and porcine parvovirus in vivo on phagocytosis and interferon mRNA expression of porcine alveolar macrophages［J］.Acta Microbiologica Sinica，2011，51（1）：105-114.（in Chinese）
［17］高金良，杨霞，陈陆，等.猪圆环病毒2型灭活疫苗种毒株的筛选［J］.中国畜牧兽医，2013，40（4）：221-224.
GAO J L，YANG X，CHEN L，et al.Screening of porcine circovirus type 2 inactivated vaccine candidate strain［J］.China Animal Husbandry ＆ Veterinary Medicine，2013，40（4）：221-224.（in Chinese）
［18］常洪涛.脑心肌炎病毒河南流行株病原学、诊断方法和疫苗的研究［D］.郑州：河南农业大学，2015.
CHANG H T.Etiology，detection methods and vaccine development for porcine and encephalomyocarditis virus wild isolate from Henan［D］.Zhengzhou：Henan Agricultural University，2015.（in Chinese）
［19］施开创，胡丽萍，郑敏，等.猪源脑心肌炎病毒GXLC株对仔猪的致病性试验［J］.中国兽医科学，2011，42（2）：138-143.
SHI K C，HU L P，ZHENG M，et al.Pathogenic experiment of porcine encephalomyocarditis virus GXLC strain to piglets［J］.Chinese Veterinary Science，2011，42（2）：138-143.（in Chinese）
［20］LARUE R，MYERS S，BREWER L，et al.A wild-type porcine encephalomyocarditis virus containing a short poly（C）tract is pathogenic to mice，pigs，and cynomolgus macaques［J］.J Virol，2003，77（17）：9136-9146.
［21］顾金燕，邢刚，雷静，等.猪圆环病毒2型与猪圆环病毒相关性系统疾病的回顾及展望［J］.生物工程学报，2015，31（6）：880-891.
GU J Y，XING G，LEI J，et al.Porcine circovirus type 2 and PCV2-systemic disease-a review［J］.Chinese Journal of Biotechnology，2015，31（6）：880-891.（in Chinese）
［22］CRAIGHEAD J E，PERALTA P H，MURNANE T G，et al.Oral infection of swine with the encephalomyocarditis virus［J］.J Infec Dis，1963，112：205-212.

[1]	LI Zhiqiang, YAN Shuping, JI Xiaoxia, WANG Kai, ZHANG Yuanshu. Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1676-1684.
[2]	LI Zhiqiang, YAN Shuping, JI Xiaoxia, WANG Kai, ZHANG Yuanshu. Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1676-1684.
[3]	YU Kexiang, LIU Cunxia, GONG Xiao, LU Xiao, HU Feng, GUO Xiaozhen, MA Xiuli, LI Yufeng, HUANG Bing, SONG Minxun. Isolation and Identification of a Variant Strain of Reovirus from Broilers [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1039-1047.
[4]	YU Kexiang, LIU Cunxia, GONG Xiao, LU Xiao, HU Feng, GUO Xiaozhen, MA Xiuli, LI Yufeng, HUANG Bing, SONG Minxun. Isolation and Identification of a Variant Strain of Reovirus from Broilers [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1039-1047.
[5]	LAI Xing, CHEN Qingju, LU Changwen, MA Yajun, TANG Zhiru. The Effects of Dietary Chlorogenic Acid and Hesperidin on Growth Performance and Intestinal Function in Weaned Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 570-580.
[6]	SHEN Xiaojuan, LI Jingjiao, BIAN Jiang, ZHAO Tingting, HUA Xiuguo, CUI Li. Metagenomics Analysis of Feces from Diarrhea Piglets Reveals the Viral Composition and Epidemic Characteristics of Coronavirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 611-619.
[7]	LAI Xing, CHEN Qingju, LU Changwen, MA Yajun, TANG Zhiru. The Effects of Dietary Chlorogenic Acid and Hesperidin on Growth Performance and Intestinal Function in Weaned Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 570-580.
[8]	SHEN Xiaojuan, LI Jingjiao, BIAN Jiang, ZHAO Tingting, HUA Xiuguo, CUI Li. Metagenomics Analysis of Feces from Diarrhea Piglets Reveals the Viral Composition and Epidemic Characteristics of Coronavirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 611-619.
[9]	GAO Zhao, HU Jiao, LIU Xiu-fan. Study on the Different Interactions between PA Protein of Two H5N1 Avian Influenza Viruses with Markedly Different Pathogenicity in Mice and Host Proteins [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1952-1960.
[10]	GAO Zhao, HU Jiao, LIU Xiu-fan. Study on the Different Interactions between PA Protein of Two H5N1 Avian Influenza Viruses with Markedly Different Pathogenicity in Mice and Host Proteins [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1952-1960.
[11]	GENG Zheng-ying, SHI Lin-xin, LIU Ping, LU Wen-qing. Effects of Dietary Supplemented with Clostridium Butyricum on Growth Performance, Antioxidant Capacity and Immune Function in Weaned Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1651-1660.
[12]	HAO Fei, MAO Li, LI Wen-liang, LI Ji-zong, YANG Lei-lei, ZHANG Wen-wen, SUN Min, ZHONG Chun-yan, LIU Mao-jun, JIANG Jie-yuan. Identification and Pathogenicity of One Caprine Herpesvirus 1 Strain [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1797-1802.
[13]	GENG Zheng-ying, SHI Lin-xin, LIU Ping, LU Wen-qing. Effects of Dietary Supplemented with Clostridium Butyricum on Growth Performance, Antioxidant Capacity and Immune Function in Weaned Piglets [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1651-1660.
[14]	HAO Fei, MAO Li, LI Wen-liang, LI Ji-zong, YANG Lei-lei, ZHANG Wen-wen, SUN Min, ZHONG Chun-yan, LIU Mao-jun, JIANG Jie-yuan. Identification and Pathogenicity of One Caprine Herpesvirus 1 Strain [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1797-1802.
[15]	GAO Xiao-long, WEI Hai-tao, SONG Yan-jun, WANG Yue, YIN Yu-qing, WANG Lin, MEI Li, FENG Xiao-yu. Progress Research of Influenza D Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1327-1333.

Analysis of Pathogenicity in Piglets Concurrent Infection with Encepholomyocarditis Virus under Immunosuppression Caused by Porcine Circovirus Type 2

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments