一株肉鸡呼肠孤病毒变异株的分离鉴定

doi:10.11843/j.issn.0366-6964.2019.05.014

Abstract

Abstract:

This experiment was conducted to study the pathogenicity and genome variation of broiler reovirus isolates in Shandong Province. In 2017, a virus was isolated from commercial broilers with tarsal joint swelling and severe bleeding in Weifang, Shandong, and identified by RT-PCR and electron microscopy. And the virus was returned to commercial broilers. The whole genome of the isolated strain was amplified by using18 pairs of primers, then sequenced, and its genetic evolution was analyzed. Serum cross neutralization test was carried out between the isolated strain and the classical strain S1133. Our results showed that a strain of avian reovirus (named WF17) was isolated. The clinical symptoms could be completely replicated in commercial broilers and the virus could be re-isolated from experimental chickens. The genome of the isolate fully conformed to the genomic structure of avian reovirus. And the main antigen (σC protein) gene was closest to 918 strain from Taiwan Province, with 92.7% homology, and 55.9% homology with S1133 strain. The antigen-related index (R value) between WF17 strain and S1133 strain was only 0.19. At present, commercialized vaccine based on S1133 strain can not effectively protect variant avian reovirus strains.

CLC Number:

S852.659.4

YU Kexiang, LIU Cunxia, GONG Xiao, LU Xiao, HU Feng, GUO Xiaozhen, MA Xiuli, LI Yufeng, HUANG Bing, SONG Minxun. Isolation and Identification of a Variant Strain of Reovirus from Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1039-1047.

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Isolation and Identification of a Variant Strain of Reovirus from Broilers

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