血管紧张素转换酶2在仔猪空肠中的分布表达及其与物质转运的关系

doi:10.11843/j.issn.0366-6964.2019.08.017

Abstract

Abstract: This study was conducted to explore the correlation among the angiotensin-converting enzyme (ACE2), the neutral amino acid transporter B⁰AT1 and the glucose transporters SGLT-1 and GLUT-2 by studying their distribution and expression in the intestines of piglets of different ages. Weaning piglets were used as the experiment animals. The middle jejunum tissues of piglets at 21 and 35 days of age were collected. The distribution and expression of ACE2 in the jejunum of piglets, and the expression of B⁰ AT1, SGLT-1 and GLUT-2 mRNA in jejunal tissues of piglets at different days were determined by Immunohistochemistry, Western blot, real-time PCR and HPLC. By detecting the types and content of free amino acids in plasma, the relationship between these indexes was analyzed. The results showed that ACE2 was mainly located in the folds and villi of the small intestine, and was expressed significantly in the brush border, serosal layer and muscle layer of jejunal epithelial cells; As the piglets grew older, the mRNA expressions of ACE2, B⁰AT1 and SGLT-1 and the content of serum neutral amino acids all increased, while the mRNA expression of GLUT-2 showed no significant change. These results indicated that ACE2 existed in jejunum of piglets, and ACE2 was related to the transport of amino acids and other substances in intestine. This fact provides the basis for the exploration of the unknown new functions of ACE2.

CLC Number:

S852.23

LI Zhiqiang, YAN Shuping, JI Xiaoxia, WANG Kai, ZHANG Yuanshu. Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1676-1684.

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References

[1] DONOGHUE M, HSIEH F, BARONAS E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9[J]. Circ Res, 2000, 87(5):E1-E9.
[2] TIPNIS S R, HOOPER N M, HYDE R, et al. A human homolog of angiotensin-converting enzyme cloning and functional expression as a captopril-insensitive carboxypeptidase[J]. J Biol Chem, 2000, 275(43):33238-33243.
[3] HARMER D, GILBERT M, BORMAN R, et al. Quantitative mRNA expression profiling of ACE2, a novel homologue of angiotensin converting enzyme[J]. FEBS Lett, 2002, 532(1-2):107-110.
[4] DANILCZYK U, SARAO R, REMY C, et al. Essential role for collectrin in renal amino acid transport[J]. Nature, 2006, 444(7122):1088-1091.
[5] CAMARGO S M R, SINGER D, MAKRIDES V, et al. Tissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations[J]. Gastroenterology, 2009, 136(3):872-882.
[6] BINDOM S M, HANS C P, XIA H J, et al. Angiotensin I-converting enzyme type 2(ACE2) gene therapy improves glycemic control in diabetic mice[J]. Diabetes, 2010, 59(10):2540-2548.
[7] PUTNAM K, SHOEMAKER R, YIANNIKOURIS F, et al. The renin-angiotensin system:a target of and contributor to dyslipidemias, altered glucose homeostasis, and hypertension of the metabolic syndrome[J]. Amer J Physiol-Heart Circul Physiol, 2012, 302(6):H1219-H1230.
[8] 王珊珊, 黄瑜,张树坤,等. 血管紧张素转化酶2(ACE2)的基因克隆及在仔猪体内的表达分布[J]. 中国兽医学报, 2013, 33(10):1579-1583.
WANG S S, HUANG Y, ZHANG S K, et al. Expression and distribution of angiotensin-converting enzyme 2(ACE2) in tissue of piglets[J]. Chinese Journal of Veterinary Science, 2013, 33(10):1579-1583. (in Chinese)
[9] 权素玉, 王睿杰,孙俊杰,等. 血管紧张素转化酶2和中性氨基酸转运载体B0AT1在不同日龄猪肠道中的表达差异[J]. 畜牧与兽医, 2015, 47(4):36-39.
QUAN S Y, WANG R J, SUN J J, et al. Different expression of angiotensin converting enzyme 2 and the neutral amino acid transporter B0AT1 in the intestinal tissues of different growth stage pigs[J]. Animal Husbandry & Veterinary Medicine, 2015, 47(4):36-39. (in Chinese)
[10] 郭丽敏, 张雪莲,杨金奎. 血管紧张素转换酶2在大鼠胰腺组织中的表达[J]. 基础医学与临床, 2007, 27(5):490-494.
GUO L M, ZHANG X L, YANG J K. Expression of angiotensin-converting enzyme 2 in rat pancreas[J]. Basic & Clinical Medicine, 2007, 27(5):490-494. (in Chinese)
[11] 张树坤, 姜雪元,谢正露,等. 高精料饲喂产生脂多糖对奶山羊血浆游离氨基酸分配的影响及其机制[J]. 畜牧兽医学报, 2013, 44(3):413-418.
ZHANG S K, JIANG X Y, XIE Z L, et al. Effect and mechanism of lipopolysaccharide on distribution of plasma free amino acid in dairy goats fed high concentrate feed[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(3):413-418. (in Chinese)
[12] LI W H, MOORE M J, VASILIEVA N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus[J]. Nature, 2003, 426(6965):450-454.
[13] SINGER D, CAMARGO S M. Collectrin and ACE2 in renal and intestinal amino acid transport[J]. Channels, 2011, 5(5):410-423.
[14] HASHIMOTO T, PERLOT T, REHMAN A, et al. ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation[J]. Nature, 2012, 487(7408):477-481.
[15] 车炼强, 张克英,丁雪梅,等. 免疫应激对仔猪肠道发育及胰高血糖素样肽-2分泌的影响[J]. 畜牧兽医学报, 2009, 40(5):676-682.
CHE L Q, ZHANG K Y, DING X M, et al. Effect of immune challenge on gut development and GLP-2 secretion of piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2009, 40(5):676-682. (in Chinese)
[16] 姜发亭. 断奶仔猪腹泻原因分析及防控措施[J]. 中国畜牧兽医文摘, 2013, 29(1):140.
JIANG F T. Cause analysis and prevention and control measures of diarrhea in Weaned Piglets[J]. Chinese Animal Husbandry and Veterinary Digest, 2013, 29(1):140. (in Chinese)
[17] 黄骞. 肠上皮细胞谷氨酰胺载体B⁰AT1/ASCT2的克隆表达及严重创伤后的功能调控研究[D]. 上海:第二军医大学, 2007.
HUANG Q. The cloning and functional characterization of glutamine transporter B⁰AT1/ASCT2 in intestinal epithelial cell and the functional regulation after severe trauma[D]. Shanghai:The Second Military Medical University, 2007. (in Chinese)
[18] JANDO J, CAMARGO S M, HERZOG B, et al. Expression and regulation of the neutral amino acid transporter B⁰AT1 in rat small intestine[J]. PLoS One, 2017, 12(9):e0184845.
[19] FAIRWEATHER S J, BRÖER A, SUBRAMANIAN N, et al. Molecular basis for the interaction of the mammalian amino acid transporters B⁰AT1 and B⁰AT3 with their ancillary protein collectrin[J]. J Biol Chem, 2015, 290(40):24308-24325.
[20] KOWALCZUK S, BRÖER A, TIETZE N, et al. A protein complex in the brush-border membrane explains a Hartnup disorder allele[J]. FASEB J, 2008, 22(8):2880-2887.
[21] PERLOT T, PENNINGER J M. ACE2-From the renin-angiotensin system to gut microbiota and Malnutrition[J]. Microb Infect, 2013, 15(13):866-873.
[22] 王修启, 谭会泽,束刚,等. 鸡肠道SGLT1和GLUT2 mRNA表达的组织特异性研究[J]. 畜牧兽医学报, 2006, 37(1):12-17.
WANG X Q, TAN H Z, SHU G, et al. Study on tissue-specific expression of SGLT1 and GLUT2 mRNA in chicken intestine[J]. Acta Veterinaria et Zootechnica Sinica, 2006, 37(1):12-17. (in Chinese)
[23] WONG T P, DEBNAM E S, LEUNG P S, et al. Involvement of an enterocyte renin-angiotensin system in the local control of SGLT1-dependent glucose uptake across the rat small intestinal brush border membrane[J]. J Physiol, 2007, 584(2):613.
[24] BERNARDI S, TIKELLIS C, CANDIDO R, et al. ACE2 deficiency shifts energy metabolism towards glucose utilization[J]. Metabolism, 2015, 64(3):406-415.
[25] KIHARA M, SATO K, HASHIMOTO T, et al. Expression of endothelial nitric oxide synthase is suppressed in the renal vasculature of angiotensinogen-gene knockout mice[J]. Cell Tissue Res, 2006, 323(2):313-320.
[26] HE F, WU C L, LI P, et al. Functions and signaling pathways of amino acids in intestinal inflammation[J]. BioMed Res Int, 2018, 2018:9171905.
[27] VICKERS C, HALES P, KAUSHIK V, et al. Hydrolysis of biological peptides by human angiotensin-converting enzyme-related carboxypeptidase[J]. J Biol Chem, 2002, 277(17):14838-14843.
[28] MA K, HU Y J, SMITH D E. Influence of fed-fasted state on intestinal PEPT1 expression and in vivo pharmacokinetics of glycylsarcosine in wild-type and Pept1 knockout mice[J]. Pharm Res, 2012, 29(2):535-545.
[29] VUILLE-DIT-BILLE R N, CAMARGO S M, EMMENEGGER L, et al. Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors[J]. Amino acids, 2015, 47(4):693-705.
[30] SHIMIZU M. Multifunctions of dietary polyphenols in the regulation of intestinal inflammation[J]. J Food Drug Anal, 2017, 25(1):93-99.
[31] CHHABRA K H, XIA H J, PEDERSEN K B, et al. Pancreatic angiotensin-converting enzyme 2 improves glycemia in angiotensin Ⅱ-infused mice[J]. Amer J Physiol Endocrinol Metab, 2013, 304(8):E874-E884.
[32] KASSIRI Z, ZHONG J C, GUO D, et al. Loss of angiotensin-converting enzyme 2 accelerates maladaptive left ventricular remodeling in response to myocardial infarction[J]. Circ Heart Fail, 2009, 2(5):446-455.

Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport

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