变异黄芪内生真菌种群分布特征

doi:10.11843/j.issn.0366-6964.2014.12.021

Abstract

Abstract:

Endophyte were isolated from Astragalus variabilis samples from Tengeli of Inner Mongolia to investigate whether endophyte exist in this plant and to assess the diversity and distribution of endophyte.Surface sterilization of plant materials was taken to isolate and purify the endophyte，and species and genus of endophyte were identified by the traditional morphology and rDNA-ITS sequence analysis.The result showed that there were many kinds of endophyte in the Astragalus variabilis samples collected in Tengeli of Inner Mongolia.Under all the conditions of sterilization，the isolation rates of leaf was highest (110.00%)，and the flower was lowest (45.00%).321 strains of endophyte were isolated from 3 different tissues of Astragalus variabilis，which belonged to 17 genera，11 families，7 orders，and 5 classes.Undifilum oxytropis was the dominant species in this plant with the relative frequency 47.98%，followed with Xylaria sp.(16.51%).There was an obviously difference in quantity，species and distribution of the endophyte between different parts of Astragalus variabilis.Leaf was the most vulnerable to infection and colonization by the endophyte，and the diversity was the maximum in stem of Astragalus variabilis.

Key words: Astragalus variabilis, endophyte, isolation and identification, species distribution

CLC Number:

S859.87

CAO Dan-dan，LU Hao，ZHAO Bao-yu，XUE Rui-xu，QUAN Hai-yun，YANG Xiao-wen，LIU Xiao-xue，YAN Du-jian. The Species Distribution Characteristics of Endophyte in Astragalus variabilis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2057-2066.

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The Species Distribution Characteristics of Endophyte in Astragalus variabilis

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