一株猫泛白细胞减少症病毒的分离鉴定及其VP2和NS1基因的变异分析

doi:10.11843/j.issn.0366-6964.2019.05.022

Abstract

Abstract:

To analyze genetic variation of feline panleukopenia virus (FPLV), in this study, a fecal sample was collected from a cat infected FPLV in Beijing and was used to isolate FPLV by CRFK cells, and identified by hemagglutination assay (HA), molecular biology. The results showed that cytopathic effects could be observed in CRFK cells. The diameter of virus particle about 25 nm was observed by electron microscope. The HA blood coagulation rate was 1:256. The full genome was cloned, sequenced and analyzed. The results showed that the isolated virus had a genome of 5 118 bp, phylogenetic tree of VP2 showed that FPLV BJ04 strain and KX434462 strain were on the same small branch. The isolated virus, according to VP2 gene homology analysis, shared from 99.1% to 99.5% homology with reference strains, and NS1 gene shared from 99.0% to 99.5%. Animal regression test results also suggested that clinical symptoms and pathological changes were observed in the challenge group. In this study, an epidemic strain of FPLV in Beijing was successfully isolated, and the genetic variation of VP2 and NS1 gene were analyzed. The results provide basic information for the better prevention and control of FPLV.

CLC Number:

S852.659.2

LIU Qi, SHI Lijun, LIANG Lin, ZHANG Lingling, YUAN Weifeng, LIANG Ruiying, LI Jinxiang, CUI Shangjin. Isolation and Identification of a Feline Panleukopenia Virus and Genetic Variation Analysis of Its VP2 and NS1[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1106-1112.

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Isolation and Identification of a Feline Panleukopenia Virus and Genetic Variation Analysis of Its VP2 and NS1

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