ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (5): 1074-1080.doi: 10.11843/j.issn.0366-6964.2018.05.023

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Antimicrobial Susceptibility Analysis and ESBL Gene Detection of Staphylococcus aureus Isolated at Animal Farms in Chongqing

CHEN Yao, DING Hong-lei, HE Ying, MU Hao, WANG Li-min, WANG Hao-ju*   

  1. Laboratory of Veterinary Lemology, Southwest University, Chongqing 400715, China
  • Received:2017-10-18 Online:2018-05-23 Published:2018-05-23

Abstract:

This paper aims at understanding the information about the antimicrobial resistance and the existence of extended-spectrum β-lactamases (ESBL) genes in livestock-associated Staphylococcus aureus (S. aureus) at some animal farms of Chongqing. One thousand three hundred and seventy-one samples were collected from animal farms. After pretreating, enriching and culturing. The S. aureus specific gene nuc was amplified from the suspected colonies, and the antimicrobial susceptibility of the identified S. aureus was tested. Meanwhile, the ESBL genes were amplified. Results were as follows:Eighty-nine S. aureus clinical strains were isolated and identified from 1 371 samples, including 25 pig-originated strains, 32 chicken-originated strains, 6 dairy cattle-originated strains, 10 goat-originated strains, and 16 rabbit-originated strains. The isolates were most resistant to penicillins, and also more resistant to tetracyclines, macrolides, and quinolones. Except for 2 rabbit-originated strains, other isolates exhibited varying degrees of multi-drug resistance, defined as resistance to 2 to 21 antimicrobials. The most prevalent multi-drug resistant strains were pig-originated. The ESBL genes detection result showed that 86 (96.6%) organisms contained blaTEM-1a. The S. aureus isolates were resistant to tested antimicrobials seriously, and most of them carried TEM-1a type ESBL.

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