5-HT对断奶仔鼠应激腹泻时肠道抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2014.12.022

Abstract

Abstract:

This study aimed to explore the relationship between 5-HT level and antioxidant function in the weaning stress-diarrhea intestine and further revealed the role of 5-HT in the gastrointestinal tract function.Forty 21-day-old weaned mice were divided into four treatment groups：Control，PCPA Control (block the synthesis of 5-HT)，Stress-diarrhea and PCPA+ stress-diarrhea.Plasma 5-HT was determined using ELISA kit.Intestinal oxidation products and antioxidant enzyme activity were determined by spectrophotometry.The results showed：(1) Weaning stress-diarrhea significantly increased plasma 5-HT concentration by 23.42% (P=0.009) compared with control group.Pretreated with PCPA decreased，plasma 5-HT concentration which had no significant difference in normal mice (P=0.200)，yet totally prevented the increase in 5-HT levels in weaning stress-diarrheal mice (P=0.006).(2) Oxidative product (MDA) level was significantly increased in intestine of stress-diarrhea group，and it increased by 2.70 time and 3.06 time than that of control group especially in duodenum and jejunum (P=0.000)；GSH-PX，CAT，SOD and T-AOC activities appeared decrease in a different extent in intestine of stress-diarrhea group.GSH-PX，SOD and T-AOC activities in duodenum were respectively reduced by 70.59% (P=0.000)，59.15% (P=0.001) and 53.01% (P=0.000).SOD activity in jejunum was significantly reduced by 71.05% (P=0.000) compared with that of control group.GSH-PX，CAT and SOD activities in ileum were respectively reduced by 72.33% (P=0.000)，50.82% (P=0.000)，73.86% (P=0.000).CAT activity in colon decreased significantly to 0.46±0.09 U•mg^－1 (from 0.85±0.06 U•mg^－1).(3) Pretreated with PCPA in the weaning stress-diarrheal mice could promote the intestinal antioxidant function，but this did not bring the mice to recovery.The results suggested that weaning stress-diarrhea could cause serious injury of intestinal antioxidant function，while blocking the synthesis of 5-HT could effectively improve its antioxidant function and relieve diarrhea symptom.

Key words: serotonin, weaning stress-diarrhea, intestine, antioxidant function

CLC Number:

S852.33

HAN Ya-nan，YANG Chen-yu，WANG Zi-xu，CHEN Yao-xing，SUN Jia-yi,QIN Zhuo-ming，CAO Jing，DONG Yu-lan . Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2067-2073.

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Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice

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