犀角地黄汤对脂多糖复制大鼠发热模型下丘脑IL-1β和PGE2的影响

doi:10.11843/j.issn.0366-6964.2013.07.022

Abstract

Abstract:

The aim of this study was to investigate the effect and mechanisms of Xijiao Dihuang Decotion（XJDH）on Lipopolysaccharide (LPS) -induced fever in rats. One hundred and twenty rats were randomly allotted into four groups: Control group, LPS group, Aspirin group and XJDH group. There were 30 rats in each group. LPS group, Aspirin group and XJDH group were reproduced by intraperitoneal injection of LPS 100 μg·kg^-1, then Aspirin group and XJDH group were drenched with Aspirin (0.04 g·mL^－1，10 mL·kg^－1 in drug delivery according to body weight) and XJDH (1.6 g·mL^－1，10 μg·kg^－1 in drug delivery according to body weight) respectively；Control group and LPS group were drenched with saline. The basal body temperature was measured before LPS was injected and then 8 h later, 2 h after administration, 6 Hypothalamus samples were collected in each group at the point of 2, 3, 5 and 8 h. IL-1β and PGE₂ were measured. The results showed that, compared with LPS group, the temperature in XJDH group were significantly improved; Compared with the control group, IL-1β of LPS group was significantly increased (P<0.05) at 2 h and 3 h, PGE₂ of LPS group was significantly increased (P<0.05) at 2 h; Compared with LPS group, XJDH significantly lowered the levels of IL-1β and PGE₂ (P<0.05). The above results suggest that XJDH significantly inhibited LPS-induced fever in Rats. The mechanism of initial antipyretic may be related to the inhibition of IL-1β in Hypothalamus, and reduce the release of PGE₂.

Key words: Xijiao Dihuang decotion, LPS, fever, IL-1β, PGE₂

CLC Number:

S853.9

DONG Hong, ZHANG Tao, HU Ge, JIANG Dai-xun, WANG Jian-fang, LI Jia, MU Xiang. Effect of Xijiao Dihuang Decotion on IL-1β and PGE₂ in Lipopolysaccharide-induced Fever in Rats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1155-1159.

References

［1］陶香玲. 夏季高热病的防治措施［J］. 中国动物保健，2011（9）：47-48.

［2］张成聪，贺永建，陈军，等. 解热灵对发热性疾病的疗效试验［J］. 畜禽业，2006（14）：56-58.

［3］王颖，姜娇，杨莉. 犀角地黄汤的临床应用近况［J］. 中国中医骨伤科杂志，2009，11(17)：263-265.

［4］YAO X J, YIN J A, XIA Y F, et al. Puerain exerts antipyretic effect on lipopolysaccharide-induced fever in rats involving inhibiton of pyrogen progen production from macrophages ［J］. J Ethnopharmacol, 2012, 141(1)：322-330.

［5］MOKRA Z D, JAVORKA M, JAVORKA K, et al. Lipopolysaccharide-induced fever alters Hering-Breuer reflex in anesthetized rats ［J］. J Thermal Biol, 2012, 37(7)：475-478.

［6］JIA L L, LI R, MA J, et al. Effects of Bai-Hu decoction on fever induced by lipopolysaccharide ［J］. Kaohsiung J Med Sci, 2013, 29(3)：128-132.

［7］左泽平，王志斌，郭玉东，等. 常用大鼠发热模型研究［J］. 中国比较医学杂志，2012，22(2)：52-57.

［8］屈飞，徐镜，崔艳茹，等. 麻杏石甘汤对LPS 诱导的大鼠发热模型的影响［J］. 中国实验方剂学杂志，2012，18(8)：178-181.

［9］HARDEN M L，PLESSIS I，POOLE S，et al. Interleukin (IL)-6 and IL-1β act synergistically within the brain to induce sickness behavior and fever in rats ［J］. Brain Behav Immun，2008，22：838-849.

［10］HARDEN M L，PLESSIS I，ROTH J. Differences in the relative involvement of peripherally released interleukin (IL)-6, brain IL-1β and prostanoids in mediating lipopolysaccharide-induced fever and sickness behavior ［J］. Psychoneuroendocrinology，2011，36(5)：608-622.

［11］HAMILTONA K L，FRANKLINB T M，ROY S，et al. Prenatal opiate exposure attenuates LPS-induced fever in adult rats: Role of interleukin-1β ［J］. Brain Res, 2007, 1133(1)：92-99.

［12］MINANO F J，SANCIBRIAN M，VIZCAINO M，et al. Macrophage inflammatory protein-1: unique action on the hypothalamus to evoke fever ［J］. Brain Res Bull，1990，24(6)：849-852.

［13］SOARES D M, VEIGA-SOUZA F H, FABRICIO A S C, et al. CCL3/Macrophage inflammatory protein-1α induces fever and increases prostaglandin E2 in cerebrospinal fluid of rats: Effect of antipyretic drugs ［J］. Brain Res, 2006, 1109(1)：83-92.

［14］ENGBLOM D, EK M, SAHA S, et al. Prostaglandins as inflammatory messengers across the blood-brain-barrier ［J］. J Mol Med，2002，80：5-15.

［15］CLARK M B. The cytokine-prostaglandin cascade in fever production: fact or fancy ［J］. J Therm Biol，2004，29：359-368.

［16］云水，刘凤华，许剑琴，等. 膏芩口服液对内毒素诱导小型猪发热的影响［J］. 畜牧兽医学报，2011，42(8)：1181-1186.

［17］王海明，仇伟. 犀角地黄汤方义探析［J］. 中国中医急症，2009，18(9)：1461-1462.

［18］许俊杰，孟庆棣. 古典解热方对家兔体温的影响［J］. 中药通报，1986，11(1): 51.

[1]	HU Yongxin, ZHAO Yonggang, ZHANG Yongqiang, LIU Fuxiao, FAN Xiaoxu, WU Xiaodong, WANG Zhiliang. Construction and Identification of Replication Defective Recombinant Adenovirus Expressing ASFV P72 Protein [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1635-1641.
[2]	HU Yongxin, ZHAO Yonggang, ZHANG Yongqiang, LIU Fuxiao, FAN Xiaoxu, WU Xiaodong, WANG Zhiliang. Construction and Identification of Replication Defective Recombinant Adenovirus Expressing ASFV P72 Protein [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1635-1641.
[3]	SHAO Dan, SONG Pengjie, YAN Baoqi, WU Xiaohu, WANG Dongsheng, LI Yajuan, ZHANG Shidong, YAN Zuoting. Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1493-1499.
[4]	SHAO Dan, SONG Pengjie, YAN Baoqi, WU Xiaohu, WANG Dongsheng, LI Yajuan, ZHANG Shidong, YAN Zuoting. Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1493-1499.
[5]	PENG Zhong, LIANG Wan, AI Weicheng, WANG Fei, HUA Lin, TANG Xibiao, CHEN Huanchun, WU Bin. Genotypical Characteristics of Swine Pasteurella multocida in China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1064-1072.
[6]	PENG Zhong, LIANG Wan, AI Weicheng, WANG Fei, HUA Lin, TANG Xibiao, CHEN Huanchun, WU Bin. Genotypical Characteristics of Swine Pasteurella multocida in China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1064-1072.
[7]	ZOU Wenshu, YIN Peng, JIN Na, GAO Qian, LIU Fenghua. α-Mangostin Alleviates LPS-induced Inflammation by Inhibiting TLR4/NF-κB Signaling [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 431-438.
[8]	ZOU Wenshu, YIN Peng, JIN Na, GAO Qian, LIU Fenghua. α-Mangostin Alleviates LPS-induced Inflammation by Inhibiting TLR4/NF-κB Signaling [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 431-438.
[9]	LIU Chang, TANG Xin, ZHANG Wen-jing, ZHAO Chun-yang, GUO Ai-zhen, HU Chang-min. 6-Bromo-Indirubin-3'-Oxime Inhibits Apoptosis of Mouse Mammary Epithelial Cell Induced by Lipopolysaccharide [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2753-2761.
[10]	LIU Chang, TANG Xin, ZHANG Wen-jing, ZHAO Chun-yang, GUO Ai-zhen, HU Chang-min. 6-Bromo-Indirubin-3'-Oxime Inhibits Apoptosis of Mouse Mammary Epithelial Cell Induced by Lipopolysaccharide [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2753-2761.
[11]	GAO Shan-dian, WANG Ji-dong, DU Jun-zheng, ZHENG Fu-ying, TIAN Zhan-cheng, YIN Hong. The Complete Genome Sequence Determination and Evolutionary Analysis of Bovine Ephemeral Fever Virus Strain HN1/2012 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2223-2231.
[12]	GAO Shan-dian, WANG Ji-dong, DU Jun-zheng, ZHENG Fu-ying, TIAN Zhan-cheng, YIN Hong. The Complete Genome Sequence Determination and Evolutionary Analysis of Bovine Ephemeral Fever Virus Strain HN1/2012 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2223-2231.
[13]	HUANG Niu, LI Huan, HE Liu-qin, TIAN Jun-quan, CUI Zhi-jie, YAO Kang. The Effects of Glutamine on the Immunity in the Intestinal Mucosa of Weaned Piglets with Lipopolysaccharide Challenge [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 752-760.
[14]	HUANG Niu, LI Huan, HE Liu-qin, TIAN Jun-quan, CUI Zhi-jie, YAO Kang. The Effects of Glutamine on the Immunity in the Intestinal Mucosa of Weaned Piglets with Lipopolysaccharide Challenge [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 752-760.
[15]	LIANG Wu-long，FANG Jia，LIN Zhi，ZHENG Min-ping，BAO Chang-lei，WANG Tao，ZHANG Yan-ming. Classical Swine Fever Virus Entry into ST Cells by Clathrin-mediated Endocytosis Pathway [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 140-149.

Effect of Xijiao Dihuang Decotion on IL-1β and PGE₂ in Lipopolysaccharide-induced Fever in Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Effect of Xijiao Dihuang Decotion on IL-1β and PGE2 in Lipopolysaccharide-induced Fever in Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Effect of Xijiao Dihuang Decotion on IL-1β and PGE₂ in Lipopolysaccharide-induced Fever in Rats