6-溴-靛玉红-3'-肟抑制脂多糖诱导的小鼠乳腺上皮细胞凋亡

doi:10.11843/j.issn.0366-6964.2018.12.025

Abstract

Abstract:

The aim of this study was to investigate the effects of 6-bromo-indirubin-3'-oxime(BIO) on apoptosis of mouse mammary epithelial cell induced by lipopolysaccharide (LPS) and its regulatory mechanism. The cells from the blank control group, DMSO group, BIO alone treatment group and different concentrations of BIO (5, 25, 50 nmol·L^-1 treatment) + LPS co-treatment group were collected, respectively. Apoptosis rate was analyzed by flow cytometry. Bak, Bax, Bcl-2, Bcl-xl, Caspase-3 and Caspase-8 mRNA expression were detected by qRT-PCR. Bcl-2, Caspase-3 and Caspase-8 protein expression were tested by Western blot. Results were as follows:BIO reduced the apoptosis rate of MMECs induced by LPS. BIO decreased LPS-induced activities of apoptosis genes Bak and Bax, apoptotic pathway proteins Caspase-3 and Caspase-8 (P<0.001) were also down-regulated. BIO increased the activities of anti-apoptosis genes Bcl-2 and Bcl-xl. BIO inhibited apoptosis of MMECs induced by LPS through regulating Bax, Bak, Bcl-2, Bcl-xl, Caspase-3 and Caspase-8 activity.

CLC Number:

S857.26

LIU Chang, TANG Xin, ZHANG Wen-jing, ZHAO Chun-yang, GUO Ai-zhen, HU Chang-min. 6-Bromo-Indirubin-3'-Oxime Inhibits Apoptosis of Mouse Mammary Epithelial Cell Induced by Lipopolysaccharide[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2753-2761.

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6-Bromo-Indirubin-3'-Oxime Inhibits Apoptosis of Mouse Mammary Epithelial Cell Induced by Lipopolysaccharide

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