输卵管上皮细胞共培养对小鼠早期胚胎发育阻滞的影响

doi:10.11843/j.issn.0366-6964.2013.07.023

Abstract

Abstract:

This experiment was conducted to study the effect of oviduct epithelial cells co-culture on the development of early embryos, and explored the mechanisms of oviduct epithelial cells co-culture on overcoming the developmental arrest in mouse. Mouse oviduct epithelial cell line was purified and used to co-culture the zygotes, H₂O₂ contents in embryos were measured by DCHFDA, MTT was used to measure the activity of epithelial cells, RT-PCR was applied to test the transcriptions of CAT and GPX genes. Oviduct epithelial cells co-culture can significantly improve the early 2-cell and 4-cell rates (P＜0.05), the blastocyst rate was also increased (P＞0.05). Oviduct epithelial cells co-culture can decrease the H₂O₂content in early 2-cell embryos (P<0.05). Also there was a deposit of H₂O₂ in the arrested embryos, the rates of typeⅠand Ⅱarrested embryos were decreased（P>0.05），type Ⅲ was increased（P＜0.05）by oviduct epithelial cells co-culture. On the other side, the cellular activity was improved (P＞0.05) when embryos developed to early 2-cell phase, at the same time, transcriptions of CAT and GPX were increased（P>0.05）. Those results indicated that oviduct epithelial cell co-culture can improve the development of embryos by cell-cell contact to decrease the reactive oxygen species content in embryos, overcome developmental arrest and promote embryo development.

Key words: oviduct epithelial cells, co-culture, early embryos, arrest, ROS

CLC Number:

S814.8

ZHUANG Li-li, WU Jun-bo, HU De-bao, CAI Li-juan, ZHANG Ye, LI Zhong-shu, FANG Nan-zhu. Effect of Oviduct Epithelial Cells Co-culture on the Developmental Arrest of Early Embryos in Mouse[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1160-1166.

References

［1］TADROS W, LIPSHITZ H D. The maternal-to-zygotic transition: a play in two acts ［J］. Development, 2009, 136: 3033-3042.

［2］LEQUARRE A S, MARCHANDISE J, MOREAU B, et al. Cell cycle duration at the time of maternal zygotic transition for in vitro produced bovine embryos ［J］. Biol Reprod, 2003,69: 1707-1713.

［3］LOPES S, JURISICOVA A, SUN J G, et al. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa ［J］. Hum Reprod, 1998, 13: 896-900.

［4］李福祥,惠宏襄.氧自由基对基因表达的调节机理［J］.生命科学,1999, S1：28-31.

［5］YANG H W, HWANG K J, KWON H C, et al. Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos［J］. Hum Reprod, 1998, 13: 998-1002.

［6］GOTO K, NODA Y, MORI T, et al. Increased generation of reactive oxygen species in embryos cultured in vitro ［J］. Free Radical Biol Med,1993, 15:69-75.

［7］BETTS D H, MADAN P. Permanent embryo arrest: molecular and cellular concepts ［J］. Mol Hum Reprod, 2008, 4:445-453.

［8］GARDINER C S, SALMEN J J, BRANDT C J, et al. Glutathione is present in reproductive tract secretions and improves development of mouse embryos after chemically-induced glutathione depletion ［J］. Biol Reprod,1998, 59: 431-436.

［9］SCHOEN J,BONDZIO A,TOPP K, et al. Establishment and characterization of an adherent pure epithelial cell line derived from the bovine oviduct ［J］. Theriogenology, 2008, 69: 536-545.

［10］SUN Y, ZHANG J, LI C，et al. Interaction between neurotrophin 4 and gonadotrophin in bovine oviducts ［J］.Theriogenology, 2012,78: 39-48.

［11］GARDINER C S, SALMEN J J, BRANDT C J, et al. Glutathione is present in reproductive tract secretions and improves development of mouse embryos after chemically-induced glutathione depletion ［J］. Biol Reprod, 1998, 59: 431-436.

［12］MIESSEN K, SHARBATI S, EINSPANIER R, et al. Modelling the porcine oviduct epithelium: A polarized in vitro system suitable for long-term cultivation ［J］.Theriogenology,2011, 76: 900-910.

［13］谭秀文，马所峰，刘新勇.不同物种输卵管上皮细胞培养、纯度检测及支持小鼠胚胎发育的能力［J］.畜牧兽医学报，2006, 37（9）：878-882.

［14］RIZOS D, RAMIREZ M A, PINTADO B, et al. Culture of bovine embryos in intermediate host oviducts with emphasis on the isolated mouse oviduct ［J］. Theriogenology, 2010,73: 777-785.

［15］XU J, CHEUNG T M, CHAN S T, et al. Human oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos ［J］. Fertil Steril, 2000,74: 1215-1219.

［16］WEGNER C C, CARSON D D. Mouse uterine stromal cells secrete a 30-kilodlaton protein in response to co-culture with uterine epithelial cells ［J］. Endocirnology, 1992,131(6):2565-2572.

［17］于建宁，邹晓庭，刘进国.山羊卵泡颗粒细胞和输卵管上皮细胞共培养对山羊外受精胚胎发育的影响［J］.中国畜牧杂志, 2004, 2: 25-27.

［18］REISCHL J, PRELLE K, SCHOL H, et al. Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelia cells in vitro ［J］. Cell Tissue Res, 1999 , 296(2): 371-383.

［19］LEE K F, YAO Y Q, KWOK K L, et al. Early developing embryos affect the gene expression patterns in the mouse oviduct ［J］.Biochem Biophys Res Commun, 2002, 292(2):564-570.

［20］FAZELI A, AFFARA N A, HUBANK M, et al. Sperm-induced modification of the oviductal gene expression profile after natural insemination in mice ［J］.Biol Reprod, 2004, 71: 60-65.

［21］GEORGIOU A S, SOSTARIC E, WONG C H, et al. Gametes alter the oviductal secretory proteome ［J］.Mol Cell Proteomics, 2005, 4:1785-1796.

［22］DESAI N, GOLDFARB J. Co-culture human embryos maybe subjected to widely different microenvironments: pattern of growth factor/cytokine release by Vero cells using the co-culture interval ［J］. Hum Reprod, 1998,13: 1600-1605.

［23］WATSON A J, WESTHUSIN M E, WINGER Q A. IGF paracrine and autocrine interactions between conceptus and oviduct ［J］. Reprod Fertil Suppl,1999, 54: 303-315.

［24］JOO B S, KIM M K, NA Y J, et al. The mechanism of action of co-culture on embryo development in the mouse model: direct embryo-to-cell contact and the removal of deleterious components ［J］. Fertil Steril, 2001,75: 193-199.

［25］AL-GUBORY K H, FOWLER P A F,GARREL C. The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes ［J］. Int J Biochem Cell Biol, 2010, 42: 1634-1650.

［26］PIERCE G B, PARCHMENT R E , LEWELLYN A L. Hydrogen peroxide as a mediator of programmed cell death in the blastocyst ［J］. Differentiation, 1991, 46: 181-186.

［27］LEE KF, YEUNG W S B. Gamete/embryo-oviduct interactions: implications on in vitro culture ［J］. Hum Fertil, 2006, 9: 137-143.

［28］KÖLLE S, REESE S, KUMMER W. New aspects of gamete transport,fertilization,and embryonic development in the oviduct gained by means of live cell imaging ［J］. Theriogenology, 2010, 73: 786-795.

［29］CHEONG A W Y, LEE Y L, LIU W M. Oviductal microsomal epoxide hydrolase(EPHX1) reduces reactive oxygen species (ROS)level and enhances preimplantation mouse embryo development ［J］. Biol Reprod, 2009, 81: 126-132.

[1]	HU Wenping, TANG Jishun, ZHANG Zhuangbiao, LA Yongfu, LIU Qiuyue, DI Ran, WANG Xiangyu, CHU Mingxing. Progress of Izumo1 and Juno in Mammalian Fertilization [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1737-1745.
[2]	YANG Xin, LI Shuo, LI Yawei, CHEN Wei, FAN Qi, ZHENG Chunhong, GAO Jingwei, HUANG Qi, ZHOU Rui, LIU Jie. Characterization and Elimination of the Effect of Sucrose on Ban Qing Granules TLC Identification [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1702-1708.
[3]	YANG Xin, LI Shuo, LI Yawei, CHEN Wei, FAN Qi, ZHENG Chunhong, GAO Jingwei, HUANG Qi, ZHOU Rui, LIU Jie. Characterization and Elimination of the Effect of Sucrose on Ban Qing Granules TLC Identification [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1702-1708.
[4]	YU Yan, GU Yu, WANG Chengdong, HU Jing, YANG Aining, YANG Qiao, LI Desheng, ZHANG Hemin, DENG Linhua, LING Shanshan, ZUO Zhicai, PENG Guangneng, ZHONG Zhijun, MA Xiaoping. Dynamic Changes of Dectin-1, TLR-2,TLR-4 and Cytokines in Mice Infected with Microsporum gypseum [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1449-1457.
[5]	YU Yan, GU Yu, WANG Chengdong, HU Jing, YANG Aining, YANG Qiao, LI Desheng, ZHANG Hemin, DENG Linhua, LING Shanshan, ZUO Zhicai, PENG Guangneng, ZHONG Zhijun, MA Xiaoping. Dynamic Changes of Dectin-1, TLR-2,TLR-4 and Cytokines in Mice Infected with Microsporum gypseum [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1449-1457.
[6]	SHEN Songwei, WANG Zeyuan, FAN Weifeng, ZHU Changchun, LU You, SHI Baolan, LIU Lei, SHEN Haifeng, JIAO Kuhua, QIN Tao, CHEN Sujuan, PENG Daxin, LIU Xiufan. Antigen Difference Analysis of H9 Subtype Avian Influenza Viruses Isolated in East China during 2017-2018 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1230-1238.
[7]	SHEN Songwei, WANG Zeyuan, FAN Weifeng, ZHU Changchun, LU You, SHI Baolan, LIU Lei, SHEN Haifeng, JIAO Kuhua, QIN Tao, CHEN Sujuan, PENG Daxin, LIU Xiufan. Antigen Difference Analysis of H9 Subtype Avian Influenza Viruses Isolated in East China during 2017-2018 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1230-1238.
[8]	JIN Xin, ZHANG Man, YANG Yinfeng. Syk is Involved in the Expression of SBD-1 in Ovine Ruminal Epithelial Cells Induced by Saccharomyces cerevisiae Mannan [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1082-1090.
[9]	JIN Xin, ZHANG Man, YANG Yinfeng. Syk is Involved in the Expression of SBD-1 in Ovine Ruminal Epithelial Cells Induced by Saccharomyces cerevisiae Mannan [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1082-1090.
[10]	ZHANG Jie, CHEN Dayong, ZHAO Juan, WANG Xiaojuan, YANG Hui, LIU Shuying. The Establishment of Co-culture System of Sheep Trophoblast Cells and Endometrial Luminal Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 562-569.
[11]	WANG Xianmei, WU Jiajia, LI Yi, LIU Qun, LIU Jing. Clone and Expression of Enolase in Sarcocystis gigantea [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 670-676.
[12]	ZHANG Jie, CHEN Dayong, ZHAO Juan, WANG Xiaojuan, YANG Hui, LIU Shuying. The Establishment of Co-culture System of Sheep Trophoblast Cells and Endometrial Luminal Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 562-569.
[13]	WANG Xianmei, WU Jiajia, LI Yi, LIU Qun, LIU Jing. Clone and Expression of Enolase in Sarcocystis gigantea [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 670-676.
[14]	HAO Baocheng, SONG Xiangdong, GAO Yan, WANG Xuehong, LIU Yu, CHEN Keyuan, LIANG Yan, HU Yuyao, XING Xiaoyong, WEN Fengqin, HU Yonghao, LIANG Jianping. Analysis of the Biosynthesis Pathway of Produced SW by Endophytic Fungi from Locoweed Using Nitrosoguanidine Mutation and High Throughput Sequencing Technology [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 169-182.
[15]	WEN Fengqin, XIANG Haitao, HAO Baocheng, XING Xiaoyong, BAO Shijun, HU Yonghao. Isolation and Identification of Corynebacterium Xerosis Isolated from Yak Liver [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 227-232.

Effect of Oviduct Epithelial Cells Co-culture on the Developmental Arrest of Early Embryos in Mouse

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments