α-倒捻子素通过抑制TLR4/NF-κB信号通路缓解LPS诱导IEC-6细胞的炎症

doi:10.11843/j.issn.0366-6964.2019.02.022

Abstract

Abstract:

The aim of this study was to prove that α-mangostin can alleviate the lipopolysaccharide (LPS)-induced inflammation of IEC-6 cells and reveal its mechanism. In this study, we constructed an LPS-induced inflammation model in IEC-6 cells in vitro, and examined whether LPS-induced inflammation was reduced by α-mangostin by means of the methods of rheumatology, ELISA, Q-PCR, and Western blot. The results showed that pretreatment with 5 μmol·L^-1 α-mangostin significantly reduced the secretion of prostaglandin (PG) E2, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and the expression of COX-2, IL-6, TNF-α, IL-1β mRNA induced by LPS in IEC-6 cells (P<0.05). By investigating the inflammatory-related signaling pathway, NF-κB, we found that α-mangostin significantly inhibited the activation of TLR4 mRNA and NF-κB-related protein phosphorylated IκBα (pIκBα) and phosphorylated p65 (pp65) (P<0.05). In summary, α-mangostin can inhibit LPS-induced inflammation of IEC-6 cells through the TLR4/NF-κB signaling pathway and may be a potential treatment for inflammatory diseases.

CLC Number:

S853.7

ZOU Wenshu, YIN Peng, JIN Na, GAO Qian, LIU Fenghua. α-Mangostin Alleviates LPS-induced Inflammation by Inhibiting TLR4/NF-κB Signaling[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 431-438.

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α-Mangostin Alleviates LPS-induced Inflammation by Inhibiting TLR4/NF-κB Signaling

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