百里香酚对脂多糖诱导的子宫内膜上皮细胞炎性反应的抑制作用研究

doi:10.11843/j.issn.0366-6964.2019.07.019

Abstract

Abstract: In order to investigate the anti-inflammatory effect of thymol on endometritis, the inflammatory model was established in LPS-induced goat endometrial epithelial cells (gEECs)with thymol treantment. The secretion of NO and cytokines were measured by NO kit and ELISA kits respectively in LPS-induced gEECs. Gene transcription of IL-1β, TLR4, and NF-κB were detected by RT-PCR respectively in LPS-induced gEECs. The results showed that thymol (100, 50, 25 μg·mL^-1) inhibited the secretion of TNF-α, IL-1β, IL-6, NO, and PGE2 to a significant extent in LPS-induced gEECs (P<0.01). In addition, thymol markedly decreased gene transcription of IL-1β, TLR4, and NF-κB in LPS-induced gEECs (P<0.01). These results demonstrated that thymol exerted anti-inflammatory effect through inhibiting the level of inflammatory cytokines, which possibly involved in the inhibition of TLR4/NF-κB signaling pathway.

CLC Number:

S853.74

SHAO Dan, SONG Pengjie, YAN Baoqi, WU Xiaohu, WANG Dongsheng, LI Yajuan, ZHANG Shidong, YAN Zuoting. Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1493-1499.

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References

[1] LEBLANC S J, DUFFIELD T F, LESLIE K E, et al. Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows[J]. J Dairy Sci, 2002, 85(9):2223-2236.
[2] WILLIAMS E J, FISCHER D P, NOAKES D E, et al. The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow[J]. Theriogenology, 2007, 68(4):549-559.
[3] ZHAO H X, ZHAO J L, SHEN J Z, et al. Prevalence and molecular characterization of fluoroquinolone resistance in Escherichia coli isolates from dairy cattle with endometritis in China[J]. Microb Drug Resist, 2014, 20(2):162-169.
[4] NAGOOR MEERAN M F, JAGADEESH G S, SELVARAJ P. Thymol attenuates inflammation in isoproterenol induced myocardial infarcted rats by inhibiting the release of lysosomal enzymes and downregulating the expressions of proinflammatory cytokines[J]. Eur J Pharmacol, 2015, 754:153-161.
[5] NAGOOR MEERAN M F, JAVED H, AL TAEE H, et al. Pharmacological properties and molecular mechanisms of thymol:prospects for its therapeutic potential and pharmaceutical development[J]. Front Pharmacol, 2017, 8:380.
[6] OMRAN S M, ESMAEILZADEH S, RAHMANI Z. Laboratory study of anticandidal activity of thyme, pennyroyal and lemon essential oils by micro dilution method[J]. Jundishapur J Microbiol, 2010, 3(4):161-167.
[7] ZHOU E S, FU Y H, WEI Z K, et al. Thymol attenuates allergic airway inflammation in ovalbumin (OVA)-induced mouse asthma[J]. Fitoterapia, 2014, 96:131-137.
[8] PATIL D, DHANESHWAR S, KADAM P. Diacerein-thymol prodrug:in vivo release and pharmacological screening in experimental models of osteoarthritis in Wistar rats[J]. Inflamm Allergy Drug Targets, 2014, 13(6):393-405.
[9] WU H C, JIANG K F, YIN N N, et al. Thymol mitigates lipopolysaccharide-induced endometritis by regulating the TLR4- and ROS-mediated NF-κB signaling pathways[J]. Oncotarget, 2017, 8(12):20042-20055.
[10] LIANG D J, LI F Y, FU Y H, et al. Thymol inhibits LPS-stimulated inflammatory response via down-regulation of NF-κB and MAPK signaling pathways in mouse mammary epithelial cells[J]. Inflammation, 2014, 37(1):214-222.
[11] LAN D, ZHANG S D, YANG D J, et al. Cytotoxicity and apoptosis induction of weisiensin B isolated from Rabdosia weisiensis C. Y. Wu in human hepatoma cells[J]. J Asian Nat Prod Res, 2008, 10(11):1055-1062.
[12] LI W S, FU K Q, LV X P, et al. Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway[J]. Int Immunopharmacol, 2015, 28(1):695-699.
[13] BRAGA P C, DAL SASSO M, CULICI M, et al. Anti-inflammatory activity of thymol:inhibitory effect on the release of human neutrophil elastase[J]. Pharmacology, 2006, 77(3):130-136.
[14] CHAUHAN A K, JAKHAR R, PAUL S, et al. Potentiation of macrophage activity by thymol through augmenting phagocytosis[J]. Int Immunopharmacol, 2014, 18(2):340-346.
[15] DIVATE R D, CHUNG Y C. In vitro and in vivo assessment of anti-inflammatory and immunomodulatory activities of Xylaria nigripes mycelium[J]. J Funct Foods, 2017, 35:81-89.
[16] DU L, LI J K, ZHANG X T, et al. Pomegranate peel polyphenols inhibits inflammation in LPS-induced RAW264. 7 macrophages via the suppression of MAPKs activation[J]. J Funct Foods, 2018, 43:62-69.
[17] WILLIAMS E J, HERATH S, ENGLAND G C W, et al. Effect of Escherichia coli infection of the bovine uterus from the whole animal to the cell[J]. Animal, 2008, 2(8):1153-1157.
[18] WU H C, ZHAO G, JIANG K F, et al. Plantamajoside ameliorates lipopolysaccharide-induced acute lung injury via suppressing NF-κB and MAPK activation[J]. Int Immunopharmacol, 2016, 35:315-322.
[19] WU H C, ZHAO G, JIANG K F, et al. Puerarin exerts an antiinflammatory effect by inhibiting NF-κB and MAPK activation in Staphylococcus aureus-induced mastitis[J]. Phytother Res, 2016, 30(10):1658-1664.
[20] RIELLA K R, MARINHO R R, SANTOS J S, et al. Anti-inflammatory and cicatrizing activities of thymol, a monoterpene of the essential oil from Lippia gracilis, in rodents[J]. J Ethnopharmacol, 2012, 143(2):656-663.
[21] KIM I H, KANG H G, JEONG J K, et al. Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis[J]. Theriogenology, 2014, 82(3):427-432.
[22] FERRARI L, KRANE M K, FICHERA A. Inflammatory bowel disease surgery in the biologic era[J]. World J Gastrointest Surg, 2016, 8(5):363-370.
[23] YANG X, SHI B, LI L, et al. Death receptor 6(DR6) is required for mouse B16 tumor angiogenesis via the NF-κB, P38 MAPK and STAT3 pathways[J]. Oncogenesis, 2016, 5(3):e206.
[24] MASLANKA T, CHROSTOWSKA M, OTROCKA-DOMAGALA I, et al. Prostaglandin E₂ exerts the proapoptotic and antiproliferative effects on bovine NK cells[J]. Res Vet Sci, 2016, 107:80-87.
[25] SHELDON I M, CRONIN J, GOETZE L, et al. Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle[J]. Biol Reprod, 2009, 81(6):1025-1032.
[26] MEI X T, XU D H, XU S K, et al. Novel role of Zn(Ⅱ)-curcumin in enhancing cell proliferation and adjusting proinflammatory cytokine-mediated oxidative damage of ethanol-induced acute gastric ulcers[J]. Chem-Biol Interact, 2012, 197(1):31-39.
[27] SUFFNESS M, PEZZUTO J M. Assays related to cancer drug discovery[M]//HOSTETTMANN K. Methods in Plant Biochemistry:Assays for Bioactivity. London:Academic Press, 1990:71-133.
[28] TURNBULL C M, MARCARINO P, SHELDRAKE T A, et al. A novel hybrid aspirin-NO-releasing compound inhibits TNFalpha release from LPS-activated human monocytes and macrophages[J]. J Inflamm (Lond), 2008, 5:12.
[29] KASIMANICKAM R, KASIMANICKAM V, KASTELIC J P. Mucin 1 and cytokines mRNA in endometrium of dairy cows with postpartum uterine disease or repeat breeding[J]. Theriogenology, 2014, 81(7):952-958.
e2.
[30] ZHANG Y, IGWE O J. Lipopolysaccharide (LPS)-mediated priming of toll-like receptor 4 enhances oxidant-induced prostaglandin E₂ biosynthesis in primary murine macrophages[J]. Int Immunopharmacol, 2018, 54:226-237.
[31] DEVI S M, KURREY N K, HALAMI P M. In vitro anti-inflammatory activity among probiotic Lactobacillus species isolated from fermented foods[J]. J Funct Foods, 2018, 47:19-27.
[32] DUARTE L J, CHAVES V C, DOS SANTOS NASCIMENTO M V P, et al. Molecular mechanism of action of Pelargonidin-3-O-glucoside, the main anthocyanin responsible for the anti-inflammatory effect of strawberry fruits[J]. Food Chem, 2018, 247:56-65.

Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells

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