可卡因-苯丙胺调控转录肽(CART)对猪卵巢卵泡颗粒细胞雌激素产生的影响

Abstract

Abstract:

The study focused on the effect of CART on FSH-induced estradiol production of porcine ovarian follicular granulose cells.Granulose cells were cultured for 7 days under different CART and FSH concentrations by Long-term culture. The growth situation in each well was observed and pictures were taken. The content of estradiol in each well was determined by sandwich ABC-ELISA. After 168 h of incubation, (1) In CART-free medium, the estrodiol levels in wells supplemented with FSH significantly increased than that of FSH-free well. Adding of 25 ng·mL^-1 of FSH resulted in the highest estrodiol level of (17 295.57±184.03) pg·mL^-1; (2) CART showed no effect on estradiol production by porcine ovarian follicular granulose cells cultured in FSHfree and 5 ng·mL^-1 FSH medium; (3) In the medium containing 5 or 25 ng·mL^-1 FSH,with the increase of CART concentration（0.01, 0.1, 1 μmol·L^-1）, the levels of estradiol concentration showed a tendency to decrease but there was no significant differences among the three groups(P>0.05). However, when FSH at 25 ng·mL^-1, CART at 0.1 or 1 μmol·L^-1, compared with the control group (FSH 25 ng·mL^-1, CART 0 μmol·L^-1), estradiol secreted by follicular granulose cells was suppressed markedly(P<0.05). The result of statistical analysis demonstrated no significant interactive effect between FSH and CART (P>0.05), which needs further investigation. (4) When the medium contained 50 ng·mL^-1 of FSH, the increase of CART concentration showed no significant influence on estradiol concentration in different groups (P>0.05). The production of follicular estradiol in pig was induced by FSH ; when the concentration of FSH were 25 and 50 ng·mL^-1, CART inhibited the estradiol production by porcine follicular granulosa cells, but no significant inhibitory effects.The result indicate that CART maybe not a main local negative regulatory factor in pig follicular development.

CLC Number:

S828
S814.1

LI Peng-fei,YUE Wen-bin, LI Fu-lu, HUANG Yang, SUN Jin-yan, ZHU Zhi-wei, YU Xiu-ju, HE Jun-ping, FAN Rui-wen, REN You-she, LV Li-hua. Effects of CART on Estradiol Production of Pig Ovarian Follicular Granulosa Cells in vitro Culture[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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References

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Effects of CART on Estradiol Production of Pig Ovarian Follicular Granulosa Cells in vitro Culture

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