荷斯坦奶牛肝脏组织中与泌乳时期及繁殖力相关的基因共表达网络构建

doi:10.11843/j.issn.0366-6964.2024.02.011

摘要/Abstract

摘要： 旨在运用加权基因共表达网络分析技术(weighted gene co-expression network analysis, WGCNA)筛选出荷斯坦奶牛(Bos taurus)肝脏组织中与泌乳时期及繁殖力相关的基因。本研究采用GEO数据库中的GSE62159数据集,该数据集分别包含高繁殖力(分泌到配种的平均时间间隔为85.6 d,n=24)和低繁殖力(分泌到配种的平均时间间隔为113.8 d,n=24)健康荷斯坦母牛在妊娠后期、泌乳初期和泌乳中期的肝脏组织转录组数据,使用R语言中的WGCNA包对该数据集进行共表达分析。将所得到的模块与不同泌乳时期以及不同繁殖力进行关联分析,得到目标模块,再依据连接度选择出排名前30的基因作为枢纽基因(Hub 基因),对Hub基因进行功能富集分析,使用String网站构建出模块的蛋白互作网络(PPI),并使用Cytoscape软件筛选出Hub基因与蛋白互作分析(PPI)网络核心基因的交集,最终得到肝脏中与泌乳时期及繁殖力相关的目标基因。研究共得到14个模块,其中tan、greenyellow两个模块与泌乳时期相关,black模块与繁殖力相关。对hub基因进行富集分析后,发现肝脏中与泌乳早期相关基因功能主要包括:有物质的合成代谢途径、脂质脂蛋白的基因表达、蛋白质的合成到分泌等;与泌乳中期相关基因功能主要包括:疾病、机体的炎症反应、免疫反应以及急性期等;与繁殖力相关基因功能主要包括:胰岛素抵抗、子宫内膜癌以及癌症等。筛选到的12个肝脏中与泌乳早期相关的目标基因有RPN1、SEC61A1、SEC61B、SEC61G、SSR1、SSR3、STT3A、DAD1、DDOST、ERLEC1、HM13和OSTC;6个与泌乳中期相关的目标基因有ITGAL、ITGB2、LAPTM5、PTPRC、C3AR1和CTSS;4个与繁殖力相关的目标基因有:PDS5A、ROCK1、AQR和LTN1。本研究使用WGCNA、PPI、基因功能富集等生物信息学方法,鉴定得到荷斯坦奶牛肝脏中与泌乳时期、繁殖力相关的目标基因,并对目标基因进行了功能富集分析,为高繁殖力及高产奶牛培育方向的科研工作积累了理论资料。

关键词: 荷斯坦奶牛, 肝脏, 泌乳, 繁殖性能, 加权基因共表达网络分析

Abstract: The purpose of this study was to use the weighted gene co-expression network analysis (WGCNA) to screen for genes related to lactation and reproductive performance in Holstein cow liver tissue. The GSE62159 dataset from the GEO database was used and the co-expression analysis was performed using the WGCNA package in R. The dataset included transcriptome data of liver tissues from healthy Holstein cows with high reproductive performance (mean interval from parturition to insemination of 85.6 days, n=24) and low reproductive performance (mean interval from parturition to insemination of 113.8 days, n=24) during late pregnancy, early lactation, and mid-lactation. The obtained modules were associated with different lactation stages and different reproductive performance. The target module was identified, and the top 30 genes based on connectivity were selected as hub genes. Functional enrichment analysis was performed on hub genes, and a protein-protein interaction (PPI) network of the module was constructed using the String website. The intersection of hub genes and PPI network core genes was screened using Cytoscape software to obtain the target genes related to lactation and reproductive performance in the liver. A total of 14 modules were obtained, of which the tan and greenyellow modules were related to lactation stages, and the black module was related to reproductive performance. After performing functional enrichment analysis on hub genes, genes related to early lactation in the liver were mainly involved in pathways related to the synthesis of substances, gene expression of lipid lipoproteins, and protein synthesis and secretion. Genes related to mid-lactation were mainly involved in diseases, body inflammation, immune responses, and acute phases. Genes related to reproductive performance were mainly involved in insulin resistance, endometrial cancer, and cancer. Twelve target genes related to early lactation in the liver were screened, including RPN1, SEC61A1, SEC61B, SEC61G, SSR1, SSR3, STT3A, DAD1, DDOST, ERLEC1, HM13, and OSTC. Six target genes related to mid-lactation were screened, including ITGAL, ITGB2, LAPTM5, PTPRC, C3AR1, and CTSS. Four target genes related to reproductive performance were screened, including PDS5A, ROCK1, AQR, and LTN1. This study utilized bioinformatics methods including WGCNA, PPI, and gene functional enrichment to identify target genes related to lactation period and reproductive performance in the liver of Holstein cows. Functional enrichment analysis of the target genes was conducted, providing theoretical data for the research direction of breeding high reproductive performance and high milk production cows.

Key words: Holstein cows, liver, lactation, reproductive performance, weighted gene co-expression network analysis

中图分类号:

S823.91

引用本文

张志飞, 唐雪颖, 闵力, 童雄, 陈卫东, 巨向红, 李大刚. 荷斯坦奶牛肝脏组织中与泌乳时期及繁殖力相关的基因共表达网络构建[J]. 畜牧兽医学报, 2024, 55(2): 528-539.

ZHANG Zhifei, TANG Xueying, MIN Li, TONG Xiong, CHEN Weidong, JU Xianghong, LI Dagang. Construction of Gene Coexpression Network Related to Lactation Period and Fecundity in Liver Tissue of Holstein Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 528-539.

使用本文

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2024.02.011

https://www.xmsyxb.com/CN/Y2024/V55/I2/528

参考文献

[1] 张胜利,孙东晓.奶牛种业的昨天、今天和明天[J].中国畜牧业,2021(15):22-26.
ZHANG S L,SUN D X.Yesterday,today and tomorrow of dairy cattle breeding[J].China Animal Industry,2021(15):22-26.(in Chinese)
[2] 杨坤,高民,魏晓玲,等.奶牛产奶量影响因素研究进展[J].中国奶牛,2023(1):10-13.
YANG K,GAO M,WEI X L,et al.Research progress on influencing factors of milk yield of dairy cows[J].China Dairy Cattle,2023(1):10-13.(in Chinese)
[3] 汤文洁,姜冰.中国乳制品加工与消费回顾[J].乳品与人类,2023(1):10-19.
TANG W J,JIANG B.Review of processing and consumption of dairy products in China[J].Dairy and Humankind, 2023(1):10-19.(in Chinese)
[4] 孟毅,魏勇,刘洋乐,等.不同因素对奶牛繁殖性能的影响[J].今日畜牧兽医,2023,39(1):70-73.
MENG Y,WEI Y,LIU Y L,et al.Effects of different factors on Reproductive performance of Dairy cows[J].Today Animal Husbandry and Veterinary Medicine,2023,39(1):70-73.(in Chinese)
[5] 张航,杨柏高,徐茜,等.热应激影响奶牛胚胎发育作用机制的研究进展[J].畜牧兽医学报,2023,54(7):2692-2700.
ZHANG H,YANG B G,XU X,et al.Research progress on the mechanism of heat stress affecting the development of dairy cow embryos[J].Acta Veterinaria et Zootechnica Sinica,2023,54(7):2692-2700.(in Chinese)
[6] 敬盈嘉,杨婉君,杨玉东,等.奶牛围产期遭受热应激对产后产奶性能的影响[J].中国畜牧杂志,2020,56(11):168-171.
JING Y J,YANG W J,YANG Y D,et al.Effects of perinatal heat stress on postpartum milk production performance of dairy cows[J].Chinese Journal of Animal Science,2020,56(11):168-171.(in Chinese)
[7] 赵慧颖,余诗强,赵玉超,等.肝脏-脂肪组织代谢串扰在围产期奶牛脂肪肝发展中的作用机制[J].畜牧兽医学报,2023,54(10):4105-4116.
ZHAO H Y,YU S Q,ZHAO Y C,et al.Mechanism of liver-adipose tissue crosstalk in the development of fatty liver in periparturient cows[J].Acta Veterinaria et Zootechnica Sinica,2023,54(10):4105-4116.(in Chinese)
[8] 刘国文,王哲.围产期奶牛能量代谢障碍性疾病的研究进展[J].黑龙江畜牧兽医,2004(8):78-79.
LIU G W,WANG Z.Research progress on energy metabolism disorders in perinatal dairy cows[J].Heilongjiang Animal Science and Veterinary Medicine,2004(8):78-79.(in Chinese)
[9] 宝华,宋利文,张航,等.围产后期亚临床酮病对奶牛氧化应激、免疫功能和生产性能的影响[J].饲料工业,2019, 40(15):49-56.
BAO H,SONG L W,ZHANG H,et al.Effects of subclinical ketosis on oxidative stress,immune function and production performance of dairy cows in postpartum[J].Feed Industry,2019,40(15):49-56.(in Chinese)
[10] 杨翠霞.高产奶牛泌乳初期能量负平衡的危害及解决方法[J].现代畜牧科技,2018(1):39.
YANG C X.Harm and solution of negative energy balance in early lactation stage of high-yield dairy cows[J].Modern Animal Husbandry Science & Technology,2018(1):39.(in Chinese)
[11] 宋长新,雷萍,王婷.基于WGCNA算法的基因共表达网络构建理论及其R软件实现[J].基因组学与应用生物学,2013,32(1):135-141.
SONG C X,LEI P,WANG T.Gene co-expression network analysis based on wgcna algorithm-theory and implementation in R software[J].Genomics and Applied Biology,2013,32(1):135-141.(in Chinese)
[12] 高慧杰,郑惠玲.WGCNA鉴定奶山羊妊娠至泌乳期乳腺发育关键基因[J].畜牧兽医学报,2020,51(11):2679-2688.
GAO H J,ZHENG H L.Identification of key genes of mammary gland development from pregnancy to lactation in dairy goats by WGCNA[J].Acta Veterinaria et Zootechnica Sinica,2020,51(11):2679-2688.(in Chinese)
[13] 王子渲,王巧,张锦,等.基于脾脏转录组筛选北京油鸡和广明白鸡抗热应激相关功能基因[J].畜牧兽医学报,2023,54(5):1905-1914.
WANG Z X,WANG Q,ZHANG J,et al.Transcriptome based screening of functional genes related to heat stress resistance in beijing you chickens and Guangming broilers[J].Acta Veterinaria et Zootechnica Sinica,2023,54(5):1905-1914.(in Chinese)
[14] 李晓波,刘占发,刘悦,等.基于WGCNA与GSEA方法挖掘调控中卫山羊羊毛弯曲相关基因[J].畜牧兽医学报,2022,53(9):2930-2943.
LI X B,LIU Z F,LIU Y,et al.Mining genes related to wool bending of Zhongwei goat based on WGCNA and GSEA[J].Acta Veterinaria et Zootechnica Sinica,2022,53(9):2930-2943.(in Chinese)
[15] 王丽敏,靳丽娜,刘亚芳,等.基于WGCNA鉴定山羊金黄色葡萄球菌型乳腺炎关键应答基因[J].家畜生态学报,2022,43(10):71-77.
WANG L M,JIN L N,LIU Y F,et al.Identification of key response genes for S. aureus-type mastitis in goats by WGCNA[J].Acta Ecologae Animalis Domastici,2022,43(10):71-77.(in Chinese)
[16] MORAN B,CUMMINS S B,CREEVEY C J,et al.Transcriptomics of liver and muscle in Holstein cows genetically divergent for fertility highlight differences in nutrient partitioning and inflammation processes[J].BMC Genom,2016, 17(1):603.
[17] CUMMINS S B,LONERGAN P,EVANS A C O,et al.Genetic merit for fertility traits in Holstein cows:I.production characteristics and reproductive efficiency in a pasture-based system[J].J Dairy Sci,2012,95(3):1310-1322.
[18] BOTÍA J A,VANDROVCOVA J,FORABOSCO P,et al.An additional k-means clustering step improves the biological features of WGCNA gene co-expression networks[J].BMC Syst Biol,2017,11(1):47.
[19] 王郅杰,赵畅,何林,等.奶牛围产期和泌乳早期主要营养代谢病代谢轮廓的研究进展[J].黑龙江八一农垦大学学报, 2021,33(5):41-48.
WANG Z J,ZHAO C,HE L,et al.Research progress on the metabolic profile of cows during perinatal and early lactation[J]. Journal of Heilongjiang Bayi Agricultural University,2021,33(5):41-48.(in Chinese)
[20] 夏科,徐作明,郗伟斌,等.奶牛脂肪肝研究进展[J].中国乳业,2008(8):76-78.
XIA K,XU Z M,XI W B,et al.Research progress of fatty liver in dairy cows[J].China Dairy,2008(8):76-78.(in Chinese)
[21] 张雪阳,罗顺.蛋白质糖基化与疾病关系的研究进展[J].生物化工,2023,9(4):184-187.
ZHANG X Y,LUO S.Research progress on the relationship between protein glycosylation and disease[J].Biological Chemical Engineering,2023,9(4):184-187.(in Chinese)
[22] 李佳丽.蛋白质糖基化与疾病关系的研究进展[J].海南医学,2023,34(4):589-592.
LI J L.Research progress on the relationship between protein glycosylation and disease[J].Hainan Medical Journal,2023, 34(4):589-592.(in Chinese)
[23] 王闻婧.HBeAg阴性慢乙肝患者血清N-糖组学改变及补肾祛邪法抗肝纤维化临床研究[D].武汉:湖北中医药大学,2017.
WANG W J.Altered serum N-glycomics in HBeAg-negative CHB and research on the effect of invigorating kidney and eliminate pathogenic method on anti-liver fibrosis[D].Wuhan:Hubei University of Chinese Medicine,2017.(in Chinese)
[24] SEO M,LEE H J,KIM K,et al.Characterizing milk production related genes in holstein using RNA-seq[J]. Asian-australas J Anim Sci,2016,29(3):343-351.
[25] 廖志权,张瑞,唐景峰.内质网整合膜蛋白SEC62研究进展[J].生命的化学,2023,43(2):247-256.
LIAO Z Q,ZHANG R,TANG J F.Research progress of endoplasmic reticulum integrated membrane protein SEC62[J].Chemistry of Life,2023,43(2):247-256.(in Chinese)
[26] SICKING M,LANG S,BOCHEN F,et al.Complexity and specificity of Sec61-channelopathies:human diseases affecting gating of the Sec61 complex[J].Cells,2021,10(5):1036.
[27] JONES M A,NG B G,BHIDE S,et al.DDOST mutations identified by whole-exome sequencing are implicated in congenital disorders of glycosylation[J].Am J Hum Genet,2012,90(2):363-368.
[28] YUAN H,YAN L L,WU M M,et al.Analysis of the estrogen receptor-associated lncrna landscape identifies a role for ERLC1 in breast cancer progression[J].Cancer Res,2022,82(3):391-405.
[29] XIE S J,JIANG X X,QIN R S,et al.miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway[J].iScience,2021,24(11):103271.
[30] WEIHOFEN A,BINNS K,LEMBERG M K,et al.Identification of signal peptide peptidase,a presenilin-type aspartic protease[J].Science,2002,296(5576):2215-2218.
[31] 刘欣欣,严金玉,代宏宇,等.高精料日粮对奶牛肝脏细胞凋亡的影响[J].畜牧与兽医,2016,48(10):1-6.
LIU X X,YAN J Y,DAI H Y,et al.Effect of high concentrate diet on hepatic cell apoptosis in diary cows[J].Animal Husbandry & Veterinary Medicine,2016,48(10):1-6.(in Chinese)
[32] 关文怡,乔立东,张凡建.围产期奶牛血清代谢物和相关激素水平的变化规律研究[J].中国畜牧杂志,2019, 55(6):128-130.
GUAN W Y,QIAO L D,ZHANG F J.Study on changes of serum metabolites and related hormones in dairy cows during transition period[J].Chinese Journal of Animal Science,2019,55(6):128-130.(in Chinese)
[33] ZHANG J C,WANG H,YUAN C,et al.ITGAL as a prognostic biomarker correlated with immune infiltrates in gastric cancer[J].Front Cell Dev Biol,2022,10:808212.
[34] LI T G,WANG W Q,GAN W Q,et al.Comprehensive bioinformatics analysis identifies LAPTM5 as a potential blood biomarker for hypertensive patients with left ventricular hypertrophy[J].Aging,2022,14(3):1508-1528.
[35] AL BARASHDI M A,ALI A,MCMULLIN M F,et al.Protein tyrosine phosphatase receptor type C[LL] (PTPRC or CD45)[J].J Clin Pathol,2021,74(9):548-552.
[36] FU Q,ZHAO S C,YANG N,et al.Genome-wide identification,expression signature and immune functional analysis of two cathepsin S (CTSS) genes in turbot (Scophthalmus maximus L.)[J].Fish Shellfish Immunol,2020,102:243-256.
[37] LI S J,XIAO J T,YU Z Y,et al.Integrated analysis of C3AR1 and CD163 associated with immune infiltration in intracranial aneurysms pathogenesis[J].Heliyon,2023,9(3):e14470.
[38] 陈希鹃,肖喜东,吴明安.影响奶牛繁殖力的因素与对策[J].中国乳业,2020(5):49-50.
CHEN X J,XIAO X D,WU M A.Factors and countermeasures affecting the fecundity of dairy cows[J].China Dairy, 2020(5):49-50.(in Chinese)
[39] 周建平,张俊育,田文儒,等.脂肪肝影响围产期奶牛繁殖力的机理研究[J].畜牧兽医学报,1997,28(2):115-119.
ZHOU J P,ZHANG J Y,TIAN W R,et al.Study of the effect of fatty liver on fertility in periparturient cows[J].Acta Veterinaria et Zootechnica Sinica,1997,28(2):115-119.(in Chinese)
[40] 李艳华,王海浪,朱玉林,等.高产荷斯坦牛繁殖障碍的诱因分析及应对策略[J].中国奶牛,2014(15):18-22.
LI Y H,WANG H L,ZHU Y L,et al.Inducement analysis and countermeasures of reproductive obstacles in high-yielding holstein cattle[J].China Dairy Cattle,2014(15):18-22.(in Chinese)

相关文章 15

[1] 曹建华, 杨柏高, 张培培, 冯肖艺, 张航, 余洲, 牛一凡, 郝海生, 杜卫华, 朱化彬, 杨凌, 赵学明. 能量负平衡影响奶牛卵泡发育的机制[J]. 畜牧兽医学报, 2024, 55(1): 22-30.

[2] 余洲, 杨柏高, 张航, 徐茜, 张培培, 冯肖艺, 曹建华, 牛一凡, 杜卫华, 郝海生, 朱化彬, 阿布力孜·吾斯曼, 赵学明. 奶水牛发情标记物的研究进展[J]. 畜牧兽医学报, 2023, 54(9): 3623-3630.

[3] 王子渲, 王巧, 张锦, Astrid Lissette Barreto Sánchez, 郑麦青, 李庆贺, 崔焕先, 安炳星, 赵桂苹, 文杰, 李和刚. 基于脾脏转录组筛选北京油鸡和广明白鸡抗热应激相关功能基因[J]. 畜牧兽医学报, 2023, 54(5): 1905-1914.

[4] 杨初蕾, 张译元, 唐红, 郭延华, 任秀美奥, 王立民, 周平. FecB基因在绵羊繁殖性能上的研究进展[J]. 畜牧兽医学报, 2023, 54(4): 1370-1380.

[5] 余诗强, 李留学, 赵小博, 赵慧颖, 屠焰, 赵玉超, 蒋林树. 不同泌乳阶段和体细胞水平的中国荷斯坦奶牛泌乳性能差异和相关性研究[J]. 畜牧兽医学报, 2023, 54(3): 1003-1014.

[6] 刘爱瑜, 冯曼, 李永亮, 赵心念, 郭建军, 王亚男, 高玉红. 卷帘舍环境参数与奶牛泌乳性能及行为的相关性分析[J]. 畜牧兽医学报, 2023, 54(2): 617-629.

[7] 杜海东, 娜仁花. 反刍动物妊娠期和泌乳期生理代谢和微生物变化及其对子代发育的影响研究进展[J]. 畜牧兽医学报, 2023, 54(11): 4458-4467.

[8] 周福振, 周部, 代旭, 王海洋, 郭梦玲, 梁艳, 杨章平, 毛永江. 荷斯坦牛泌乳前期体况评分及其对泌乳性能和离群寿命的影响[J]. 畜牧兽医学报, 2022, 53(9): 2955-2969.

[9] 杨洋, 周子薇, 张京一, 杨硕, 王博宇, 葛楠, 林叶, 侯晓明. 奶牛SP1基因结构及对乳脂合成功能的初步分析[J]. 畜牧兽医学报, 2022, 53(9): 2970-2981.

[10] 胡立萍, 沈子亮, 王全, 于紫桐, 张琦绮, 毛永江, 杨章平, 张慧敏. 泌乳早期奶牛瘤胃微生物与牛奶脂肪酸组成的变化[J]. 畜牧兽医学报, 2022, 53(9): 3018-3028.

[11] 郭大伟, 侯思鲁, 池宇佳, 于非可, 尉啸涵, 邓倩, 肖传明, 刘晓晔, 董虹. 芪英汤和子甘汤中药复方促进母猪繁殖性能和断奶仔猪生长性能的临床研究[J]. 畜牧兽医学报, 2022, 53(6): 1994-2004.

[12] 王影, 文亮, 母童, 冯小芳, 刘佳敏, 张娟, 温万, 顾亚玲. 荷斯坦牛高、低乳脂率牛乳代谢组分析[J]. 畜牧兽医学报, 2022, 53(5): 1396-1408.

[13] 刘佳敏, 禹保军, 母童, 张迪, 冯小芳, 张娟, 王影, 温万, 顾亚玲. 奶牛乳脂代谢关键miRNAs的筛选及鉴定[J]. 畜牧兽医学报, 2022, 53(12): 4244-4257.

[14] 王川川, 母童, 冯小芳, 禹保军, 张娟, 顾亚玲. 基于转录组测序挖掘奶牛乳脂代谢关键候选基因[J]. 畜牧兽医学报, 2022, 53(10): 3421-3433.

[15] 段晨莹, 李欣, 赵羚均, 许师源, 原开敏, 董智豪, 郭冠华, 王栋. ART3抑制剂3-MBA对小鼠睾丸生精功能的影响[J]. 畜牧兽医学报, 2022, 53(10): 3470-3479.

编辑推荐

Metrics

阅读次数

全文

摘要

本文评价

摘要

参考文献

相关文章

编辑推荐

Metrics

本文评价

回顶部

荷斯坦奶牛肝脏组织中与泌乳时期及繁殖力相关的基因共表达网络构建

Construction of Gene Coexpression Network Related to Lactation Period and Fecundity in Liver Tissue of Holstein Cows

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	曹建华, 杨柏高, 张培培, 冯肖艺, 张航, 余洲, 牛一凡, 郝海生, 杜卫华, 朱化彬, 杨凌, 赵学明. 能量负平衡影响奶牛卵泡发育的机制[J]. 畜牧兽医学报, 2024, 55(1): 22-30.
[2]	余洲, 杨柏高, 张航, 徐茜, 张培培, 冯肖艺, 曹建华, 牛一凡, 杜卫华, 郝海生, 朱化彬, 阿布力孜·吾斯曼, 赵学明. 奶水牛发情标记物的研究进展[J]. 畜牧兽医学报, 2023, 54(9): 3623-3630.
[3]	王子渲, 王巧, 张锦, Astrid Lissette Barreto Sánchez, 郑麦青, 李庆贺, 崔焕先, 安炳星, 赵桂苹, 文杰, 李和刚. 基于脾脏转录组筛选北京油鸡和广明白鸡抗热应激相关功能基因[J]. 畜牧兽医学报, 2023, 54(5): 1905-1914.
[4]	杨初蕾, 张译元, 唐红, 郭延华, 任秀美奥, 王立民, 周平. FecB基因在绵羊繁殖性能上的研究进展[J]. 畜牧兽医学报, 2023, 54(4): 1370-1380.
[5]	余诗强, 李留学, 赵小博, 赵慧颖, 屠焰, 赵玉超, 蒋林树. 不同泌乳阶段和体细胞水平的中国荷斯坦奶牛泌乳性能差异和相关性研究[J]. 畜牧兽医学报, 2023, 54(3): 1003-1014.
[6]	刘爱瑜, 冯曼, 李永亮, 赵心念, 郭建军, 王亚男, 高玉红. 卷帘舍环境参数与奶牛泌乳性能及行为的相关性分析[J]. 畜牧兽医学报, 2023, 54(2): 617-629.
[7]	杜海东, 娜仁花. 反刍动物妊娠期和泌乳期生理代谢和微生物变化及其对子代发育的影响研究进展[J]. 畜牧兽医学报, 2023, 54(11): 4458-4467.
[8]	周福振, 周部, 代旭, 王海洋, 郭梦玲, 梁艳, 杨章平, 毛永江. 荷斯坦牛泌乳前期体况评分及其对泌乳性能和离群寿命的影响[J]. 畜牧兽医学报, 2022, 53(9): 2955-2969.
[9]	杨洋, 周子薇, 张京一, 杨硕, 王博宇, 葛楠, 林叶, 侯晓明. 奶牛SP1基因结构及对乳脂合成功能的初步分析[J]. 畜牧兽医学报, 2022, 53(9): 2970-2981.
[10]	胡立萍, 沈子亮, 王全, 于紫桐, 张琦绮, 毛永江, 杨章平, 张慧敏. 泌乳早期奶牛瘤胃微生物与牛奶脂肪酸组成的变化[J]. 畜牧兽医学报, 2022, 53(9): 3018-3028.
[11]	郭大伟, 侯思鲁, 池宇佳, 于非可, 尉啸涵, 邓倩, 肖传明, 刘晓晔, 董虹. 芪英汤和子甘汤中药复方促进母猪繁殖性能和断奶仔猪生长性能的临床研究[J]. 畜牧兽医学报, 2022, 53(6): 1994-2004.
[12]	王影, 文亮, 母童, 冯小芳, 刘佳敏, 张娟, 温万, 顾亚玲. 荷斯坦牛高、低乳脂率牛乳代谢组分析[J]. 畜牧兽医学报, 2022, 53(5): 1396-1408.
[13]	刘佳敏, 禹保军, 母童, 张迪, 冯小芳, 张娟, 王影, 温万, 顾亚玲. 奶牛乳脂代谢关键miRNAs的筛选及鉴定[J]. 畜牧兽医学报, 2022, 53(12): 4244-4257.
[14]	王川川, 母童, 冯小芳, 禹保军, 张娟, 顾亚玲. 基于转录组测序挖掘奶牛乳脂代谢关键候选基因[J]. 畜牧兽医学报, 2022, 53(10): 3421-3433.
[15]	段晨莹, 李欣, 赵羚均, 许师源, 原开敏, 董智豪, 郭冠华, 王栋. ART3抑制剂3-MBA对小鼠睾丸生精功能的影响[J]. 畜牧兽医学报, 2022, 53(10): 3470-3479.