噬菌体RZⅢ-3的生物学特性分析及对鸡大肠杆菌的疗效

doi:10.11843/j.issn.0366-6964.2017.11.018

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (11): 2166-2172.doi: 10.11843/j.issn.0366-6964.2017.11.018

噬菌体RZⅢ-3的生物学特性分析及对鸡大肠杆菌的疗效

王杰^1,2, 李璐璐¹, 张庆^1*

1. 山东省农业科学院畜牧兽医研究所, 济南 250100;
2. 山东大学生命科学学院, 济南 250100

收稿日期:2017-05-04 出版日期:2017-11-23 发布日期:2017-11-23
通讯作者: 张庆,E-mail:zhangqingchina@163.com
作者简介:王杰(1990-),女,山东济宁人,硕士,主要从事噬菌体基本性质和裂解机制研究,Tel:0531-88962860,E-mail:wangjier1020@163.com
基金资助:
国家自然科学基金（81271886）；山东省农业重大应用技术创新项目（SDAIT-11-09）；山东省自然科学培养基金（ZR2014CP021）

Biological Characteristics Analysis of Phage RZⅢ-3 to Escherichia coli and the Therapeutic Effect on Chicken

WANG Jie^1,2, LI Lu-lu¹, ZHANG Qing^1*

1. Animal Husbandry and Veterinary Institute, Shandong Academy of Agricultural Science, Jinan 250100, China;
2. School of Life Science, Shandong University, Jinan 250100, China

Received:2017-05-04 Online:2017-11-23 Published:2017-11-23

摘要/Abstract

摘要：

为了解决目前严重的抗生素耐药性，寻找抗生素的替代品，作者探究了大肠杆菌噬菌体RZⅢ-3的基本生物学特性及其对致病性大肠杆菌1019的治疗效果。大肠杆菌噬菌体RZⅢ-3的生物学特性分析显示其效价可达到10⁹ pfu·mL^-1，透射电镜结果显示该噬菌体属于有尾目T4噬菌体。热稳定性较好，在60℃保温1 h后噬菌体效价仍可达到10⁷ pfu·mL^-1以上。对pH值的适应范围较宽，在pH4~10内仍能保持较高的裂解活性；该噬菌体的最适pH在7.0左右。最佳感染复数（MOI）为0.1，感染宿主菌的潜伏期20 min，爆发期70 min。大肠杆菌噬菌体RZⅢ-3对大肠杆菌1019有较强的裂解能力，可使SPF鸡肠道内大肠杆菌1019的数量降低96.5%。该大肠杆菌噬菌体RZⅢ-3在治疗特定的大肠杆菌感染中具有较大的应用潜能。

Abstract:

To solve the antibiotic resistance and find substitution of antibiotics, this study explored the basic biological characteristics of Escherichia coli phage RZⅢ-3 and its therapeutic effect on SPF chicken colibacillosis. The biological characteristics of Escherichia coli phage RZⅢ-3 showed that the titer was 10⁹ pfu·mL^-1, high stability to heat, the bacteriophage titer can reach more than 10⁷ pfu·mL^-1 after incubation at 60℃ for 1 h. It had a wide adaptive range of pH, and maintained a high cleavage activity at pH 4-10. The optimum pH of the phage was about 7.0, its optimal multiplicity of infection (MOI) was 0.1, and the incubation period of infected host bacteria was about 20 min. The results of transmission electron microscopy showed that the phage belonged to the T4 phage. Escherichia coli phage RZⅢ-3 on Escherichia coli 1019 had a strong ability to crack, The number of Escherichia coli 1019 in SPF chickens can be reduced by 96.5%.This indicated that the Escherichia coli phage RZⅢ-3 has a greater potential for the treatment of specific Escherichia coli infection.

中图分类号:

Q939.48

王杰, 李璐璐, 张庆. 噬菌体RZⅢ-3的生物学特性分析及对鸡大肠杆菌的疗效[J]. 畜牧兽医学报, 2017, 48(11): 2166-2172.

WANG Jie, LI Lu-lu, ZHANG Qing. Biological Characteristics Analysis of Phage RZⅢ-3 to Escherichia coli and the Therapeutic Effect on Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2166-2172.

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噬菌体RZⅢ-3的生物学特性分析及对鸡大肠杆菌的疗效

Biological Characteristics Analysis of Phage RZⅢ-3 to Escherichia coli and the Therapeutic Effect on Chicken

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