1株感染多种致病性大肠杆菌噬菌体的生物学特性研究

doi:10.11843/j.issn.0366-6964.2022.01.028

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (1): 282-289.doi: 10.11843/j.issn.0366-6964.2022.01.028

1株感染多种致病性大肠杆菌噬菌体的生物学特性研究

林焱¹, 罗润波², 刘燕坤¹, 朱伟云^1*

1. 国家动物消化道营养国际联合研究中心, 江苏省消化道营养与动物健康重点实验室, 南京农业大学消化道微生物研究室, 南京 210095;
2. 西藏农牧学院动物科学学院, 林芝 860000

收稿日期:2021-04-21 出版日期:2022-01-23 发布日期:2022-01-26
通讯作者: 朱伟云,主要从事动物消化道微生物研究,E-mail:zhuweiyun@njau.edu.cn
作者简介:林焱(1986-),女,福建长乐人,讲师,博士,主要从事动物消化道微生物研究,E-mail:linyan@njau.edu.cn;Tel:025-84395520
基金资助:
国家自然科学基金青年基金（31802102）；江苏省自然科学基金青年基金（BK20180540）；中央高校基本业务费联合项目（KYYZ202001）

Comparison of Infection Characteristics of an Escherichia coli Phage C6 on Different Pathogenic Escherichia coli Strains

LIN Yan¹, LUO Runbo², LIU Yankun¹, ZHU Weiyun^1*

1. National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China;
2. College of Animal Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, China

Received:2021-04-21 Online:2022-01-23 Published:2022-01-26

摘要/Abstract

摘要： 旨在测定此前从断奶前仔猪粪样中分离得到的1株大肠杆菌噬菌体C6在致病性大肠杆菌上的生物学特性，并比较该噬菌体在不同致病菌上的感染特性。利用电镜形态观察和基因组测序确定其分类，用点滴法和双层平板法测定其在致病性大肠杆菌上的宿主谱，通过噬菌斑形态、最佳感染复数（MOI）、成斑率（EOP）、吸附率、一步生长曲线以及抑菌曲线等来评价噬菌体在多株致病菌上的感染特性。结果显示：噬菌体C6可感染1株非致病性大肠杆菌和4株致病性大肠杆菌，包括大肠杆菌O157（E.coli W1）、2株产志贺毒素大肠杆菌（STEC W3、STEC W5）和1株肠致病性大肠杆菌（EPEC 143）。通过比较噬菌体C6在4株致病菌上的各项感染特性指标发现：噬菌斑大小和成斑率E.coli W1>EPEC 143>STEC W3>STEC W5；吸附率E.coli W1>EPEC 143、STEC W3、STEC W5；最佳MOI EPEC 143、STEC W3、STEC W5>E.coli W1；液体LB中生长能力和抑菌能力E.coli W1>STEC W3>EPEC 143、STEC W5。噬菌体C6为T4样噬菌体，可感染多株致病性大肠杆菌，在不同致病菌上的感染特性存在差异，但均能显著抑制致病菌的生长。

关键词: 大肠杆菌噬菌体, 大肠杆菌O157, 产志贺毒素大肠杆菌, 肠致病性大肠杆菌, 感染特性, 抑菌效力

Abstract: This study aimed to determine and characterize the biological and infection characteristics of an Escherichia coli phage C6 which was previous isolated from feacal samples of pre-weaned piglet. Bacteriophage classification was confirmed by TEM observation and sequencing. Host range was determined by spot assay and double-layer agar plates method. Infecting characteristics including Optimal multiplicity of infection (MOI), efficiency of plating (EOP), adsorption rate, one-step growth curve as well as bacteriostatic curves of phage C6 were determined. Results were as follows: Escherichia coli phage C6 was a T4-like phage. Phage C6 infected a Escherichia coli O157 strain (E. coli W1), two Shiga toxin-producing Escherichia coli strains (STEC W3, STEC W5) and a enteropathogenic Escherichia coli strain (EPEC 143). The results of each infection index were shown in descending order as follows. Plaque size and EOP: E. coli W1>EPEC 143>STEC W3>STEC W5; optimal MOI: EPEC 143, STEC W3, STEC W5>E. coli W1; EOP and adsorption rate: E. coli W1>EPEC 143, STEC W3, STEC W5; phage proliferation and bacteriostatic efficacy in LB medium: E. coli W1>STEC W3>EPEC 143, STEC W5. E. coli phage C6 was a T4-like phage that infected different pathogenic E. coli strains and had significant bacteriostatic effects on pathogenic E. coli strains despite of varied infection characteristics.

Key words: Escherichia coli phage, Escherichia coli O157, Shiga toxin-producing Escherichia coli, enteropathogenic Escherichia coli, infection characteristics, bacteriostatic effect

中图分类号:

Q939.48

林焱, 罗润波, 刘燕坤, 朱伟云. 1株感染多种致病性大肠杆菌噬菌体的生物学特性研究[J]. 畜牧兽医学报, 2022, 53(1): 282-289.

LIN Yan, LUO Runbo, LIU Yankun, ZHU Weiyun. Comparison of Infection Characteristics of an Escherichia coli Phage C6 on Different Pathogenic Escherichia coli Strains[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 282-289.

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1株感染多种致病性大肠杆菌噬菌体的生物学特性研究

Comparison of Infection Characteristics of an Escherichia coli Phage C6 on Different Pathogenic Escherichia coli Strains

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