猪流行性腹泻疫苗研究进展

doi:10.11843/j.issn.0366-6964.2025.03.007

Abstract

Abstract:

The porcine epidemic diarrhea (PED) is an infectious intestinal disease caused by porcine epidemic diarrhea virus (PEDV), which results in an outbreak of diarrhea in pigs and high mortality in newborn piglet. In 2010, a highly pathogenic mutant strain of PEDV emerged and spread globally, which has presently been one of the main pathogens of porcine diarrhea and caused large losses for the global swine industry. Vaccination is the primary measure for preventing and controlling PED. Currently, available commercial PED vaccines mainly consist of traditional inactivated and attenuated vaccines. With the development of genetic engineering technologies in recent years, significant advancements have been made in the study of subunit vaccines, virus-like particle vaccines, recombinant vector vaccines, transgenic plant vaccines, and nucleic acid vaccines. The frequent variations of PEDV continues to bring new challenges to the vaccine development. Therefore, efficient vaccine development platforms and engineering vaccines are crucial for future prevention and control of PED. This article provides a systematic review of the pathogenic characteristics of PEDV and the latest advancements in vaccine research, as well as prospects for future vaccine development, aiming to provide a reference for effective prevention and control of PED.

Key words: porcine epidemic diarrhea (PED), porcine epidemic diarrhea virus (PEDV), inactivated vaccine, attenuated vaccine, engineering vaccine, nucleic acid vaccines

CLC Number:

WU Peiling, LI Yixuan, WANG Haojie, LI Yafei, LIU Shaomeng, LIU Qingyun, WANG Xiangru. Research Progress of Porcine Epidemic Diarrhea Vaccine for Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1042-1058.

Figures/Tables 10

Table 1

Information list on the issuance of the commercialized porcine epidemic diarrhoea vaccine in 2024"

毒株 Strain	疫苗类型 Vaccine type	免疫途径 Immune pathway	免疫对象 Immunization target	生产公司 Production company
TGEV华毒株+PEDV CV777株	猪传染性胃肠炎、猪流行性腹泻二联灭活疫苗	后海穴注射	母猪和断奶仔猪	山东海利生物技术股份有限公司
				四川海林格生物制药有限公司
				吉林正业生物制品股份有限公司
				江苏南农高科技股份有限公司
				广东温氏大华农生物科技有限公司
				北京华夏兴洋生物科技有限公司
				普莱柯生物工程股份有限公司
				商丘美兰生物工程有限公司
				山东滨州沃华生物工程有限公司
				哈药集团生物疫苗有限公司
				中牧实业股份有限公司江西生药厂
				成都史纪生物制药有限公司
		后海穴注射或肌肉注射	母猪和断奶仔猪	青岛蔚蓝生物股份有限公司
		后海穴注射或肌肉注射	母猪和公猪	湖南中岸生物药业有限公司
TGEV弱毒华毒株+PEDV CV777株+PoRV NX株	猪传染性胃肠炎、猪流行性腹泻、猪轮状病毒三联弱毒活疫苗	后海穴注射	母猪、新生仔猪和断奶仔猪	山东海利生物技术股份有限公司
				吉林正业生物制品股份有限公司
				哈尔滨维科生物技术有限公司
TGEV WH-1R株+PEDV AJ1102-R株	猪传染性胃肠炎、猪流行性腹泻二联弱毒活疫苗	肌肉注射	母猪、新生仔猪和断奶仔猪	武汉科前生物股份有限公司
	猪传染性胃肠炎、猪流行性腹泻二联灭活疫苗	肌肉注射、口服免疫	母猪、公猪和新生仔猪	国药集团扬州威克生物工程有限公司
		肌肉注射	母猪和新生仔猪	武汉科前生物股份有限公司
			母猪和新生仔猪	安徽东方帝维生物制品股份有限公司
			母猪、公猪和新生仔猪	山东华宏生物工程有限公司
		肌肉注射	母猪和公猪	杭州佑本动物疫苗有限公司
TGEV HB08株+PEDV ZJ08株	猪传染性胃肠炎、猪流行性腹泻二联弱毒活疫苗	肌肉注射	母猪、新生仔猪和断奶仔猪	瑞普(保定)生物药业有限公司
				中牧实业股份有限公司成都药械厂
				金宇保灵生物药品有限公司
		肌肉注射、后海穴注射、口服免疫	母猪和新生仔猪	兆丰华生物科技有限公司
		后海穴注射或肌肉注射	母猪、公猪和新生仔猪	畜科生物工程有限公司
PEDV XJ-DB2株	猪流行性腹泻灭活疫苗	肌肉注射	母猪、新生仔猪和断奶仔猪	天康生物股份有限公司
TGEV SCJY-1株+PEDV SCSZ-1株	猪传染性胃肠炎、猪流行性腹泻二联弱毒活疫苗	肌肉注射、后海穴注射、口服免疫	母猪、公猪、新生仔猪和断奶仔猪	华派生物工程集团有限公司
		肌肉注射、后海穴注射、口服免疫	母猪、新生仔猪和断奶仔猪	重庆澳龙生物制品有限公司
		肌肉注射、口服免疫	母猪、公猪和新生仔猪	吉林特研生物技术有限责任公司
		/	/	成都史纪生物制药有限公司
TGEV SD/L株+PEDV LW/L株	猪传染性胃肠炎、猪流行性腹泻二联弱毒活疫苗	肌肉注射	母猪和断奶仔猪	青岛易邦生物工程有限公司
				洛阳惠中生物技术有限公司
				齐鲁动物保健品有限公司
			母猪和公猪	杭州佑本动物疫苗有限公司
PEDV CHYJ株	猪流行性腹泻灭活疫苗	滴鼻免疫	母猪	四川海林格生物制药有限公司

Table 1

Table 2

List of the commercial vaccines for porcine epidemic diarrhea that have been clinically approved from 2018 to 2023"

毒株Strain	疫苗类型Vaccine type	批件号Approval number	有效期限Valid period
TGEV SHXB株+PEDV ZJ/15株+PDCoV LYG/14株	猪传染性胃肠炎、猪流行性腹泻、猪丁型冠状病毒三联灭活疫苗	2023036	2023/03/02-2025/03/01
PEDV CHO-3G12株	猪流行性腹泻基因工程亚单位疫苗	2023066	2022/05/31-2024/05/30
PEDV J01株+S06株+Z09株+PoRV G4+G9型	猪流行性腹泻、猪轮状病毒二联灭活疫苗	2023084	2022/01/26-2024/01/25
PEDV HB17-R株+PoRV JS01-R株	猪流行性腹泻、猪轮状病毒二联活疫苗	2023064	2020/05/19-2022/05/18
PEDV ZJ/15株	猪流行性腹泻灭活疫苗	2022064	2021/04/14-2023/04/13
PEDV HeN2017株	猪流行性腹泻灭活疫苗	2023019	2021/03/19-2023/03/18
PEDV HB17株+PoRV JS01株	猪流行性腹泻、猪轮状二联灭活疫苗	2022030	2021/03/29-2023/03/28
PDCoV AK-R株+PEDV WN-R株	猪丁型冠状病毒、猪流行性腹泻二联活疫苗	2022003	2019/05/14-2021/05/13
PEDV FJ-2013株灭活疫苗	猪流行性腹泻灭活疫苗	2020018	2018/03/05-2019/03/04
PEDV TBJS株Ⅱ型活疫苗	猪流行性腹泻Ⅱ型活疫苗	2021004	2018/01/23-2019/01/22
TGEV SD14株+PEDV HuN16株+PDCoV HeN17株	猪传染性胃肠炎、猪流行性腹泻、猪丁型冠状病毒三联灭活疫苗	2021002	2017/08/28-2018/08/27
TGEV WH-1株+PEDV AJ1102株+PDCoV CHN-HN-2014株	猪传染性胃肠炎、猪流行性腹泻、猪丁型冠状病毒三联灭活疫苗	2021003	2016/12/30-2017/12/29
PDCoV SDclone10株+PEDV KB-4clone13株	猪丁型冠状病毒、猪流行性腹泻二联灭活疫苗	2019016	2017/08/28-2018/08/27
TGEV CT130株+PEDV HX115株	猪传染性胃肠炎、猪流行性腹泻二联活疫苗	2018019	2016/12/30-2017/12/29
PEDV KB-4clone13株	猪流行性腹泻灭活疫苗	2018005	2016/09/29-2017/09/28

Table 2

Table 3

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Table 6

Table 7

Table 8

Table 9

Table 10

References 75

1	SUN R Q , CAI R J , CHEN Y Q , et al.Outbreak of porcine epidemic diarrhea in suckling piglets, China[J].Emerg Infect Dis,2012,18(1):161-163. doi: 10.3201/eid1801.111259
2	LI C , LU H Y , GENG C , et al.Epidemic and evolutionary characteristics of swine enteric viruses in South-Central China from 2018 to 2021[J].Viruses,2022,14(7):1420. doi: 10.3390/v14071420
3	BRIAN D A , BARIC R S .Coronavirus genome structure and replication[J].Curr Top Microbiol Immunol,2005,287,1-30.
4	LI W T , VAN KUPPEVELD F J M , HE Q G , et al.Cellular entry of the porcine epidemic diarrhea virus[J].Virus Res,2016,226,117-127. doi: 10.1016/j.virusres.2016.05.031
5	LI C H , LI W T , DE ESESARTE E L , et al.Cell attachment domains of the porcine epidemic diarrhea virus spike protein are key targets of neutralizing antibodies[J].J Virol,2017,91(12):e00273-17.
6	CHANG C Y , CHENG I C , CHANG Y C , et al.Identification of neutralizing monoclonal antibodies targeting novel conformational epitopes of the porcine epidemic diarrhoea virus spike protein[J].Sci Rep,2019,9(1):2529.
7	CHANG S H , BAE J L , KANG T J , et al.Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus[J].Mol Cells,2002,14(2):295-299.
8	SUN D B , FENG L , SHI H Y , et al.Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein[J].Vet Microbiol,2008,131(1-2):73-81.
9	CRUZ D J M , KIM C J , SHIN H J .Phage-displayed peptides having antigenic similarities with porcine epidemic diarrhea virus (PEDV) neutralizing epitopes[J].Virology,2006,354(1):28-34.
10	左媛媛, 徐天刚, 戈胜强, 等.我国猪流行性腹泻病毒流行现状及分子遗传演化特征[J].中国动物检疫,2022,39(9):9-17.
	ZUO Y Y , XU T G , GAO S Q , et al.Prevalence and molecular genetic evolution of porcine epidemic diarrhea virus in China[J].China Animal Health Inspection,2022,39(9):9-17.
11	WANG L Y , BYRUM B , ZHANG Y .New variant of porcine epidemic diarrhea virus, United States, 2014[J].Emerg Infect Dis,2014,20(5):917-919.
12	KWEON C H , KWON B J , JUNG T S , et al.Isolation of porcine epidemic diarrhea virus (PEDV) in Korea[J].Korean J Vet Res,1993,33(2):249-254.
13	SONG D S , OH J S , KANG B K , et al.Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain[J].Res Vet Sci,2007,82(1):134-140.
14	KWEON C H , KWON B J , LEE J G , et al.Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate[J].Vaccine,1999,17(20-21):2546-2553.
15	SATO T , TAKEYAMA N , KATSUMATA A , et al.Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo[J].Virus Genes,2011,43(1):72-78.
16	STEVENSON G W , HOANG H , SCHWARTZ K J , et al.Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences[J].J Vet Diagn Invest,2013,25(5):649-654.
17	LIN C M , SAIF L J , MARTHALER D , et al.Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains[J].Virus Res,2016,226,20-39.
18	GERDTS V , ZAKHARTCHOUK A .Vaccines for porcine epidemic diarrhea virus and other swine coronaviruses[J].Vet Microbiol,2017,206,45-51.
19	BAEK P S , CHOI H W , LEE S , et al.Efficacy of an inactivated genotype 2b porcine epidemic diarrhea virus vaccine in neonatal piglets[J].Vet Immunol Immunopathol,2016,174,45-49.
20	王明, 马思奇, 周金法, 等.猪流行性腹泻灭活疫苗的研究[J].中国畜禽传染病,1993(5):17-19.
	WANG M , MA S Q , ZHOU J F , et al.Research on inactivated vaccine against epidemic diarrhea in pigs[J].Chinese Journal of Preventive Veterinary Medicine,1993(5):17-19.
21	董世娟, 谢春芳, 司伏生, 等.猪流行性腹泻病毒免疫及疫苗研制[J].生物工程学报,2021,37(8):2603-2613.
	DONG S J , XIE C F , SI F S , et al.Immunization against porcine epidemic diarrhea virus and vaccine development[J].Chinese Journal of Biotechnology,2021,37(8):2603-2613.
22	常小云, 赵润泽, 同珂, 等.一株变异型猪流行性腹泻病毒的分离鉴定及其灭活疫苗的免疫效力试验[J].中国畜牧兽医,2020,47(11):3651-3658.
	CHANG X Y , ZHAO R Z , TONG K , et al.Isolation and identification of a variant porcine epidemic diarrhea virus and the immune efficacy of its inactivated vaccine[J].China Animal Husbandry & Veterinary Medicine,2020,47(11):3651-3658.
23	郭振刚, 胡江锋, 王婷, 等.猪流行性腹泻病毒分离鉴定及其灭活疫苗的研制[J].动物医学进展,2021,42(2):26-33.
	GUO Z G , HU J F , WANG T , et al.Isolation and identification of porcine epidemic diarrhea virus and development of inactivated vaccine[J].Progress in Veterinary Medicine,2021,42(2):26-33.
24	SINGH G , SINGH P , PILLATZKI A , et al.A minimally replicative vaccine protects vaccinated piglets against challenge with the porcine epidemic diarrhea virus[J].Front Vet Sci,2019,6,347.
25	LI M X , ZHANG Y Y , FANG Y X , et al.Construction and immunogenicity of a trypsin-independent porcine epidemic diarrhea virus variant[J].Front Immunol,2023,14,1165606.
26	XU X W , DU L P , FAN B C , et al.A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets[J].Arch Virol,2020,165(6):1299-1309.
27	HSUEH F C , CHANG Y C , KAO C F , et al.Intramuscular immunization with chemokine-adjuvanted inactive porcine epidemic diarrhea virus induces substantial protection in pigs[J].Vaccines (Basel),2020,8(1):102.
28	CHOE S , PARK G N , SONG S , et al.Efficacy of needle-less intradermal vaccination against porcine epidemic diarrhea virus[J].Pathogens,2021,10(9):1115.
29	ZHANG E , WANG J L , LI Y C , et al.Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets[J].Exp Ther Med,2020,20(2):1596-1606.
30	JANG G , WON H , LEE D U , et al.Assessment of the safety and efficacy of an attenuated live vaccine based on highly virulent genotype 2b porcine epidemic diarrhea virus in nursing piglets[J].Vet Microbiol,2019,231,120-128.
31	SCHUMACHER L , CHEN Q , FREDERICKS L , et al.Evaluation of the efficacy of an S-INDEL PEDV strain administered to pregnant gilts against a virulent Non-S-INDEL PEDV challenge in newborn piglets[J].Viruses,2022,14(8):1801.
32	WON H , LEE D U , JANG G , et al.Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus[J].J Vet Sci,2019,20(4):e32.
33	KIM D M , MOON S H , KIM S C , et al.Development of effective PEDV vaccine candidates based on viral culture and protease activity[J].Vaccines (Basel),2023,11(5):923.
34	HOU Y X , KE H Z , KIM J , et al.Engineering a live attenuated porcine epidemic diarrhea virus vaccine candidate via inactivation of the viral 2'-O-methyltransferase and the endocytosis signal of the spike protein[J].J Virol,2019,93(15):e00406-19.
35	DENG X F , BUCKLEY A C , PILLATZKI A , et al.Inactivating three interferon antagonists attenuates pathogenesis of an enteric coronavirus[J].J Virol,2020,94(17):e00565-20.
36	ZHANG G G , PENG Q , LIU S Y , et al.The glycosylation sites in RBD of spike protein attenuate the immunogenicity of PEDV AH2012/12[J].Virus Res,2024,345,199381.
37	郭涛. 猪流行性腹泻病毒COE亚单位疫苗制备及免疫原性分析[D]. 石河子: 石河子大学, 2021.
	GUO T. Preparation and immunogenicity analysis of porcine epidemic diarrhea virus COE subunit vaccine[D]. Shihezi: Shihezi University, 2021. (in Chinese)
38	CHOE S , SONG S , PIAO D C , et al.Efficacy of orally administered porcine epidemic diarrhea vaccine-loaded hydroxypropyl methylcellulose phthalate microspheres and RANKL-secreting L. lactis[J].Vet Microbiol,2020,242,108604.
39	武旺盛, 颜仁和, 仇珍珍, 等.猪流行性腹泻病毒重组S1蛋白的免疫原性评价[J].中国兽医科学,2021,51(3):305-313.
	WU W S , YAN R H , QIU Z Z , et al.Immunogenicity of recombinant S1 of porcine epidemic diarrhea virus[J].Chinese Veterinary Science,2021,51(3):305-313.
40	GUO W L , WANG C H , SONG X , et al.Immunogenicity and protective efficacy of a trimeric full-length S protein subunit vaccine for porcine epidemic diarrhea virus[J].Vaccine,2024,42(4):828-839.
41	吴松. 猪流行性腹泻病毒S1蛋白的杆状病毒表达及其免疫原性的研究[D]. 广州: 华南农业大学, 2018.
	WU S. The study on the baculovirus expression and immunogenicity of PEDV S1 protein[D]. Guangzhou: South China Agricultural University, 2018. (in Chinese)
42	CHANG C Y , HSU W T , TSAI P S , et al.Oral administration of porcine epidemic diarrhea virus spike protein expressing in silkworm pupae failed to elicit immune responses in pigs[J].AMB Express,2020,10(1):20.
43	YU J S , SREENIVASAN C , UPRETY T , et al.Piglet immunization with a spike subunit vaccine enhances disease by porcine epidemic diarrhea virus[J].NPJ Vaccines,2021,6(1):22.
44	WANG Y T , LIU S J , LI B S , et al.A novel CpG ODN compound adjuvant enhances immune response to spike subunit vaccines of porcine epidemic diarrhea virus[J].Front Immunol,2024,15,1336239.
45	LU Y , CLARK-DEENER S , GILLAM F , et al.Virus-like particle vaccine with B-cell epitope from porcine epidemic diarrhea virus (PEDV) incorporated into hepatitis B virus core capsid provides clinical alleviation against PEDV in neonatal piglets through lactogenic immunity[J].Vaccine,2020,38(33):5212-5218.
46	HSU C W , CHANG M H , CHANG H W , et al.Parenterally administered porcine epidemic diarrhea virus-like particle-based vaccine formulated with CCL25/28 chemokines induces systemic and mucosal immune protectivity in pigs[J].Viruses,2020,12(10):1122.
47	LUO C W , YAN Q H , HUANG J C , et al.Using self-assembling ADDomer platform to display B and T epitopes of type O foot-and-mouth disease virus[J].Viruses,2022,14(8):1810.
48	DU P F , YAN Q H , ZHANG X A , et al.Virus-like particle vaccines with epitopes from porcine epidemic virus and transmissible gastroenteritis virus incorporated into self-assembling ADDomer platform provide clinical immune responses in piglets[J].Front Immunol,2023,14,1251001.
49	LI L J , YIN S H , ZHOU J J , et al.Spike 1 trimer, a nanoparticle vaccine against porcine epidemic diarrhea virus induces protective immunity challenge in piglets[J].Front Microbiol,2024,15,1386136.
50	PFEILER E A , AZCARATE-PERIL M A , KLAENHAMMER T R .Characterization of a novel bile-inducible operon encoding a two-component regulatory system in Lactobacillus acidophilus[J].J Bacteriol,2007,189(13):4624-4634.
51	BERMÚDEZ-HUMARÁN L G , KHARRAT P , CHATEL J M , et al.Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines[J].Microb Cell Fact,2011,10(S1):S4.
52	ZANG Y , TIAN Y , LI Y G , et al.Recombinant Lactobacillus acidophilus expressing S₁ and S₂ domains of porcine epidemic diarrhea virus could improve the humoral and mucosal immune levels in mice and sows inoculated orally[J].Vet Microbiol,2020,248,108827.
53	ZHENG D Z , WANG X N , JU N , et al.Immune responses in pregnant sows induced by recombinant lactobacillus johnsonii expressing the COE protein of porcine epidemic diarrhea virus provide protection for piglets against PEDV infection[J].Viruses,2021,14(1):7.
54	马茹梦. 不同乳杆菌受体菌表达猪流行性腹泻病毒保护性抗原S1诱导免疫应答的比较研究[D]. 哈尔滨: 东北农业大学, 2023.
	MA R M. Comparative study on the immune response induced by the different Lactobacillus receptor bacteria with expressing the protective antigen S1 of porcine epidemic diarrhea virus[D]. Harbin: Northeast Agricultural University, 2023. (in Chinese)
55	马茹梦, 赵玉梁, 马明爽, 等.不同猪源受体菌表达猪流行性腹泻病毒保护性抗原S1诱导免疫应答的比较研究[J].畜牧兽医学报,2024,55(5):2090-2099.
	MA R M , ZHAO Y L , MA M S , et al.Comparative Study on the immune response induced by the different porcine receptor bacteria with expressing the protective antigen S1 of porcine epidemic diarrhea virus[J]. Acta Veterinaria et Zootechnica Sinica,2024,55(5):2090-2099.
56	GUO T T , GAO C , HAO J H , et al.Strategy of developing oral vaccine candidates against co-infection of porcine diarrhea viruses based on a Lactobacillus delivery system[J].Front Microbiol,2022,13,872550.
57	WANG J L , HUANG L L , MOU C X , et al.Mucosal immune responses induced by oral administration recombinant Bacillus subtilis expressing the COE antigen of PEDV in newborn piglets[J].Biosci Rep,2019,39(3):BSR20182028.
58	YU D , JIN C , RAMACHANDRAN M , et al.Adenovirus serotype 5 vectors with Tat-PTD modified Hexon and serotype 35 Fiber show greatly enhanced transduction capacity of primary cell cultures[J].PLoS One,2013,8(1):e54952.
59	DO V T , JANG J , PARK J , et al.Recombinant adenovirus carrying a core neutralizing epitope of porcine epidemic diarrhea virus and heat-labile enterotoxin B of Escherichia coli as a mucosal vaccine[J].Arch Virol,2020,165(3):609-618.
60	WANG F Q , ZHANG Q , ZHANG F Q , et al.Adenovirus vector-mediated single chain variable fragments target the nucleocapsid protein of porcine epidemic diarrhea virus and protect against viral infection in piglets[J].Front Immunol,2023,14,1058327.
61	LIU X S , ZHAO D H , ZHOU P , et al.Evaluation of the efficacy of a recombinant adenovirus expressing the spike protein of porcine epidemic diarrhea virus in pigs[J].Biomed Res Int,2019,2019,8530273.
62	SONG X , ZHOU Q , ZHANG J Q , et al.Immunogenicity and protective efficacy of recombinant adenovirus expressing a novel genotype G2b PEDV spike protein in protecting newborn piglets against PEDV[J].Microbiol Spectr,2024,12(1):e0240323.
63	KE Y , YU D Y , ZHANG F Q , et al.Recombinant vesicular stomatitis virus expressing the spike protein of genotype 2b porcine epidemic diarrhea virus: a platform for vaccine development against emerging epidemic isolates[J].Virology,2019,533,77-85.
64	WANG H , YI W C , QIN H , et al.A Genetically engineered bivalent vaccine coexpressing a molecular adjuvant against classical swine fever and porcine epidemic diarrhea[J].Int J Mol Sci,2023,24(15):11954.
65	LI W , LEI M K , LI Z F , et al.Development of a genetically engineered bivalent vaccine against porcine epidemic diarrhea virus and porcine rotavirus[J].Viruses,2022,14(8):1746.
66	PASCUAL-IGLESIAS A , SANCHEZ C M , PENZES Z , et al.Recombinant chimeric transmissible gastroenteritis virus (TGEV)—porcine epidemic diarrhea virus (PEDV) virus provides protection against virulent PEDV[J].Viruses,2019,11(8):682.
67	HO T T , TRINH V T , TRAN H X , et al.The immunogenicity of plant-based COE-GCN4pⅡ protein in pigs against the highly virulent porcine epidemic diarrhea virus strain from genotype 2[J].Front Vet Sci,2022,9,940395.
68	SOHN E J , KANG H , MIN K , et al.A plant-derived maternal vaccine against porcine epidemic diarrhea protects piglets through maternally derived immunity[J].Vaccines (Basel),2023,11(5):965.
69	尹国友. 猪流行性腹泻病毒抗原基因在番茄中的表达及动物免疫试验[D]. 新乡: 河南师范大学, 2020.
	YIN G Y. Expression of porcine epidemic diarrhea virus antigen gene in tomato and animal immunoassay[D]. Xinxiang: Henan Normal University, 2020. (in Chinese)
70	EGELKROUT E , HAYDEN C , FAKE G , et al.Oral delivery of maize-produced porcine epidemic diarrhea virus spike protein elicits neutralizing antibodies in pigs[J].Plant Cell Tissue Organ Cult,2020,142(1):79-86.
71	YIN Y , ZHU L , LIU P J , et al.Evaluation on the efficacy and immunogenicity of recombinant DNA plasmids expressing S gene from porcine epidemic diarrhea virus and VP7 gene from porcine rotavirus[J].Braz J Microbiol,2019,50(1):279-286.
72	ZHANG Y D , ZHANG X H , LIAO X D , et al.Construction of a bivalent DNA vaccine co-expressing S genes of transmissible gastroenteritis virus and porcine epidemic diarrhea virus delivered by attenuated Salmonella typhimurium[J].Virus Genes,2016,52(3):354-364.
73	SAWATTRAKOOL K , STOTT C J , BANDALARIA-MARCA R D , et al.Field trials evaluating the efficacy of porcine epidemic diarrhea vaccine, RNA (Harrisvaccine) in the Philippines[J].Trop Anim Health Prod,2020,52(5):2743-2747.
74	杨利敏, 王俊虹, 徐明国, 等.猪流行性腹泻mRNA疫苗的制备与免疫原性评价[J].生物工程学报,2023,39(7):2624-2633.
	YANG L M , WANG J H , XU M G , et al.Preparation and immunogenicity evaluation of mRNA vaccine against porcine epidemic diarrhea[J].Chinese Journal of Biotechnology,2023,39(7):2624-2633.
75	ZHAO Y X , FAN B C , SONG X , et al.PEDV-spike-protein-expressing mRNA vaccine protects piglets against PEDV challenge[J].mBio,2024,15(2):e0295823.

毒株 Strain	疫苗佐剂 Vaccine adjuvant	免疫对象 Immune target	参考文献 Reference
GF10	氢氧化铝	妊娠母猪	[22]
GS	ISA 61VG和ISA 206	妊娠母猪	[23]
CO2013	/	3周龄仔猪	[24]
rAJ1102-S2′JS2008	/	4周龄仔猪	[25]
AH2012/12	Flic和ISA 201	妊娠母猪	[26]
PT-5	CCL27、CCL28、CCL25和弗氏佐剂	5周龄仔猪	[27]
SGP-M1	Carbopol^® 940 NF聚合物	妊娠母猪	[28]
ZJ08	/	5日龄仔猪	[29]

毒株 Strain	致弱手段 Attenuated measures	免疫剂量 Immune dose	免疫对象 Immune target	参考文献 Reference
S DEL5/ORF3	Vero细胞中连续传代100次	10^6.5 TCID ₅₀·mL^-1	妊娠母猪	[30]
IL20697	Vero细胞中连续传代	10^4.0 TCID₅₀·mL^-1	妊娠母猪	[31]
Aram-P29-CA	Vero细胞中较低温度(32 ℃)下连续传代28次	10^3.0 TCID₅₀·mL^-1	妊娠母猪	[32]
CKT-7 N、CKT-7 T15、CKT-7 T15N、CKT-7 NF、CKT-7 F	在培养基中单独或混合添加(TPCK处理的胰蛋白酶、1% FBS或Na-GCDCA)的Vero细胞中连续传代180次	10^7.0 TCID₅₀·mL^-1	5日龄仔猪	[33]
KDKE4A-SYA	抑制nsp16的2′-O甲基转移酶的活性并敲除S蛋白胞内域的内吞信号序列	/	妊娠母猪	[34]
icPEDV-mute4	抑制nsp1、nsp15和nsp16蛋白的活性	/	4~7日龄仔猪	[35]
r12-N514G、r12-N556G和r12-N514+556G	用天冬酰胺氨基酸替换aa514和aa556位点的丝氨酸	10^4.5 TCID ₅₀·mL^-1	5日龄仔猪	[36]

毒株 Strain	表达系统 Expression system	表达蛋白 Expressed proteins	免疫对象 Immune target	参考文献 Reference
CH/PDS/2015	pET-32a原核表达系统	COE结构域	3月龄仔猪	[37]
CV777	pG5-S1D原核表达系统	aP2蛋白	妊娠母猪	[38]
KM242131	HEK-293T表达系统	S1亚基	断奶仔猪	[39]
AH2012/12	HEK-293T表达系统	S蛋白、S1亚基和S蛋白多表位串联的三聚体蛋白	3日龄仔猪	[40]
CT	昆虫杆状病毒表达系统	S/亚基	妊娠母猪	[41]
PT	家蚕核型多角体病毒	S蛋白	4周龄仔猪	[42]
COJS01	Bac-to-Bac杆状病毒表达系统	S蛋白	新生仔猪	[43]
CT	昆虫杆状病毒表达系统	S蛋白	30日龄仔猪	[44]

毒株 Strain	疫苗载体 Vaccine vector	表达蛋白 Expressed proteins	免疫对象 Immune target	参考文献 Reference
/	乙肝病毒核心衣壳蛋白(HBcAg)	⁷⁴⁸YSNIGVCK⁷⁵⁵	妊娠母猪	[45]
PT	P-BEVS	S、M和E蛋白	4周龄仔猪	[46]
AJ1102	ADDomer	SS2、2C10结构域和A、D位点	断奶仔猪	[48]
GS2022	Trimer-Tag	S1亚基、COE和RBD结构域	妊娠母猪	[49]

毒株 Strain	细菌载体 Bacterial vector	表达蛋白 Expressed proteins	免疫对象 Immune target	参考文献 Reference
CH-SDBZ-1-2015	嗜酸乳杆菌(Lactobacillus acidophilus)	S1亚基	妊娠母猪	[52]
HLJ-2012株	约氏乳杆菌(L. johnsonii)	COE结构域	妊娠母猪	[53]
CH/HLJ/2019	干酪乳酪杆菌(Lacticaseibacillus casei 393)、猪源副干酪乳酪杆菌(L. paracasei 27-2)、猪源罗伊氏黏液乳杆菌(Limosilactobacillus reuteri J31)和猪源约氏乳杆菌(L. johnsonii 6332)	S1亚基	新生仔猪	[54-55]
GT/2017	干酪乳酪杆菌(L. casei ATCC 393)	6Ds、COE结构域、VP4蛋白	新生仔猪	[56]
ZJ08	枯草芽胞杆菌(B. subtilis WB800)	COE结构域	仔猪	[57]

Research Progress of Porcine Epidemic Diarrhea Vaccine for Pigs

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毒株 Strain	病毒载体 Viral vector	表达蛋白 Expressed proteins	免疫对象 Immune target	参考文献 Reference
CH-SDBZ-1-2015	腺病毒	LTB和COE结构域	3周龄仔猪	[59]
SH2012-5株	腺病毒	N蛋白的单链可变片段(scvFs)	3日龄仔猪	[60]
CH/HBXT/2018	腺病毒	S蛋白	4周龄仔猪	[61]
CH/TP-4-4/2018	腺病毒	S蛋白	妊娠母猪	[62]
CHN/SHANGHAI/2012	水疱性口炎病毒	S蛋白	妊娠母猪	[63]
GX750A	猪瘟病毒	S1N和COE结构域	5周龄仔猪	[64]
YN150	猪流行性腹泻病毒	VP7蛋白	10日龄仔猪	[65]
NVSL	传染性胃肠炎病毒	S蛋白	5周龄仔猪	[66]

毒株 Strain	植物载体 Plant vector	表达蛋白 Expressed proteins	免疫对象 Immune target	参考文献 Reference
PS6	本氏烟草	COE/G2a-p2蛋白	妊娠母猪	[67]
/	本氏烟草	融合pFc2结构域的S1亚基	妊娠母猪	[68]
HeNPEDV-01株	番茄	S1亚基	仔猪	[69]
Colorado/2013	玉米	与不耐热肠毒素B亚基和树突状细胞肽融合的S蛋白	妊娠母猪	[70]

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