GreA蛋白对肠炎沙门菌生物学特性及致病力影响

doi:10.11843/j.issn.0366-6964.2024.11.032

Abstract

Abstract:

The aim is to study the effect of GreA protein on the biological characteristics and pathogenicity of Salmonella Enteritidis. Based on the parent strain ATCC13076, ΔgreA and ΔgreB single gene deletion strains and ΔgreAΔgreB double gene deletion strains were constructed by λ-Red recombinant system. Their growth and swimming motility, resistance to stressful environment, biofilm formation ability, and pathogenicity to cells and mice of the parents and the deletion strains were detected. RNA-seq was used to detect differentially expressed genes (DEGs). GreA protein negatively regulated the H₂O₂ resistance, the growth in vitro and biofilm formation ability of Salmonella Enteritidis, and positively regulated the motor ability, cell invasion and intracellular proliferation ability of Salmonella Enteritidis and its colonizing ability in mouse organs, but have no significant effect on resistance of Salmonella Enteritidis to high temperature and acid environment. GreA protein affects the expression of 107 genes, including positive regulation of inv operon, fim operon and flg operon, which are mainly related to bacterial motility and cell invasion; and negative regulation of cps operon, cbp operon and leu operon, which are related to amino acid synthesis and metabolism. GreA protein affects the environmental adaptability and pathogenicity of Salmonella Enteritidis by regulating the expression of metabolic pathways related to flagellar synthesis and cell invasion.

Key words: Salmonella Enteritidis, biological characteristics, GreA, RNA-seq

CLC Number:

S852.612

Menglei CAI, Dongxu ZHAO, Zhenggang ZHANG, Donghai LIU, Tingting JIANG, Shixuan SU, Xuemin YAN, Xiaoyang XUE, Guolin CUI. The Effect of GreA Protein on the Biological Characteristics and Pathogenicity of Salmonella Enteritidis[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5173-5182.

Figures/Tables 7

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引物 Primers	序列(5′→3′) Sequence
greAF	AGCCGGGGTAGAGTTCATTGCCCCCTACAGGAATGTTCAAGAGGTATAACGTGTAGGCTGGAGCTGCTTC
greAR	ACAATACACCAACAATTTGCGTATTGAGTACTACTTAAAGGTATTCCACCATATGAATATCCTCCTTAG
greBF	ACGTTATACCCCGTGTGCGCAATATCGACAGCAAAGGTAAATCAACGAGGTGTAGGCTGGAGCTGCTTC
greBR	ATGCCAGCCATCAGCGGGGGCTTAGGATTCTTCTTGTCTTATTTGACGCATATGAATATCCTCCTTAG
greApF	GCGGATCCGATGCAAGCTATTCCGATGACCT
greApF	CGAAGCTTAAGGTATTCCACTTTAAGCACT
greBpF	GCGGATCCGATGAAAACGCCCCTGATCAC
greBpR	CGAAGCTTTTTGACGTATTCGATCGCA

差异类型 Difference type	操纵子 Operons	功能描述 Function description
上调 Upregulated	invA-invE-invG-invF	SPI-1
	ttrC-ttrA	SPI-2
	ssaL-ssaK	SPI-2
	ssaN-ssaV-ssaM	SPI-2
	flgJ-SEN_RS09710-flgH	Flagellar L-ring
	flgD-flgC-flgB	Flagellar bas al-body rod modification
	flhA-flhE	Flagellar biosynthesis
	fliR- fliQ	Flagellar biosynthesis
	fimA-fimI-fimC	Type 1 fimbrial
	cheR-cheB-cheY	Bacterial chemotaxis protein
	motA-motB-cheA-cheW	Bacterial chemotaxis protein
	dppD-dppC	Dipeptide transport system binding protein
	argC-argB	Arginine biosynthesis
	potE- speF	Arginine and proline metabolism
下调 Downregulated	leuD-leuC-leuB	Leucine synthesis
	cpsG-cpsB-wcaI- SEN_RS10955-SEN_RS10960-gmD-wcaF	Fructose and mannose metabolism
	yedF-yedE	Anaerobic metabolism
	cysC-cysN-cysD	Purine metabolism
	SEN_RS05075-wrbA	Ubiquinone and other terpenoid-quinone biosynthesis
	sigE-sopB	SPI-1
	cbpM-cbpA	Curved DNA-binding protein

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The Effect of GreA Protein on the Biological Characteristics and Pathogenicity of Salmonella Enteritidis

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