Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (11): 5183-5190.doi: 10.11843/j.issn.0366-6964.2024.11.033
• Preventive Veterinary Medicine • Previous Articles Next Articles
Nana PENG(
), Huimin NING, Yuhao CHEN, Xinying LI, Fuqiang ZHU, Guobin YU, Wei DONG*()
Received:2023-12-22
Online:2024-11-23
Published:2024-11-30
Contact:
Wei DONG
E-mail:1625989715@qq.com;dongwei@hunau.edu.cn
CLC Number:
Nana PENG, Huimin NING, Yuhao CHEN, Xinying LI, Fuqiang ZHU, Guobin YU, Wei DONG. Competitive Blocking Effect of Salmonella Enteritis Fimbriae Recombinant Protein on Its Cell Adhesion[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5183-5190.
Fig. 1
PCR amplification of FimA-sefA gene and identification of pET28a-FimA-sefA recombinant plasmid A. FimA-sefA amplification (1, 2. Amplified fragment of FimA-sefA; M. DL5000 DNA marker); B. pET28a-FimA-sefA digestion (1. Digestion of pET28a-FimA-sefA recombinant plasmid; 2. Empty vector pET28a (+); M. DL5000 DNA marker)"
Fig. 2
Expression of target protein FimA-sefA(A), Western blot identification(B) and effect of different time on the expression of protein(C) A. Expression of target protein FimA-sefA (1. Induced total protein expression; 2. Induced expression precipitation; 3. Induced expression of supernatant; 4. Total protein of negative control; 5. Negative control precipitation; 6. Negative control supernatant; M. Protein marker); B. Western blot identification (1. FimA-sefA protein; M. Protein marker); C. Effect of different time on the expression of protein (1. Whole strain; 2-6. Time is induced under 8, 12, 16, 20, 24 h, IPTG concentration 1.0 mmol ·L-1, temperature 30 ℃; M. Protein marker)"
Fig. 3
Purification and identification of target protein FimA-sefA 1. Flow through the liquid; 2-6. Wash buffer 1, 2, 3, 4, 5; 7. Elution buffer; M. Protein marker"
Fig. 4
Immunofluorescence rendering of FimA-sefA protein, recombinant bacteria and Salmonella Enterica adhere to IPEC-J2 cells(100×) A. Salmonella Enterica; B. FimA-sefA protein; C. Recombinant bacteria; D. Negative control"
Fig. 5
Effect of FimA-sefA protein and recombinant bacteria on the adhesion of Salmonella Enterica to IPEC-J2 cells A. Recombinant protein; B. Recombinant bacteria. ****. P < 0.000 1; ***. P < 0.001; **. P < 0.01"
Fig. 6
Immunofluorescence effect of FimA-sefA protein and recombinant bacteria on Salmonella Enterica adherent IPEC-J2 cells(100×) A. Positive control; B. FimA-sefA protein; C. Recombinant bacteria; D. Negative control"
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