芦丁在BV2细胞中抗弓形虫的作用及机制分析

doi:10.11843/j.issn.0366-6964.2024.07.030

Abstract

Abstract:

This experiment was conducted to study the in vitro anti-Toxoplasma gondii (T. gondii) effect of rutin and its mechanism in BV2 cells. Firstly, the anti-T. gondii effect of rutin in BV2 cells was explored by detecting rutin cytotoxicity, T. gondii inhibition rate and proliferation by CCK8 and indirect fluorescence staining, etc. Subsequently, the cells were divided into four groups: normal group (Normal), T. gondii infected control group (T. gondii), and T. gondii infected groups with low-dose rutin (Rut 50) and T. gondii infected groups with high-dose Rutin (rut 100). Then T. gondii infection and rutin treatment experiments were performed, and the levels of antioxidant enzymes SOD, GSH and lipid oxidation product MDA, the levels of inflammatory factors TNF-α, IL-6 and IL-1β in the supernatant were detected by ELISA methods. The expression of key proteins in PI3K/AKT/Nrf2/HO-1, TLR4/NF-κB and P2X7R/NLRP3 signaling pathways in BV2 cells were detected by Western blot. The results showed that 50 and 100 μg·mL^－1 of rutin had a significant anti-T. gondii effect in BV2 cells, as the T. gondii infection rate, invasion ability and proliferation were significantly inhibited (P < 0.05). Rutin also significantly increased the SOD and GSH activities and decreased the MDA content (P < 0.05), and decreased TNF-α, IL-6 and IL-1β levels in the supernatant (P < 0.05). And it also significantly up-regulated the expression of key proteins in the antioxidant pathway PI3K/AKT/Nrf2/HO-1, and down-regulated the inflammatory pathway TLR4/NF-κB and P2X7R/NLRP3 (P < 0.05). These results indicated that rutin has a significant anti-T. gondii effect in BV2 cells in vitro, which may be related to its ability to enhance cellular antioxidant capacity through activation of the PI3K/AKT/Nrf2/HO-1 pathway, as well as inhibit the TLR4/NF-κB and P2X7R/NLRP3 pathways to attenuate inflammatory responses. These findings may provide some theoretical references for further research on the anti-T. gondii effect of rutin in vivo, as well as its protective effect in T. gondii-induced brain damage. It may also provide some theoretical basis for the development of rutin as a potential therapeutic agent for the treatment of toxoplasmosis in humans and animals.

Key words: rutin, anti-T. gondii effect, BV2 cells, antioxidant, anti-inflammatory, PI3K/AKT/Nrf2/HO-1, TLR4/NF-κB, P2X7R/NLRP3

CLC Number:

S859.7

Chengquan HAN, Jingtao LIU, Miao YU, Lizeng GUAN, Lu XU, Yueshang WANG. Study on the Anti-Toxoplasma gondii Effect of Rutin in BV2 Cells and Its Mechanism[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3119-3131.

Figures/Tables 9

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Study on the Anti-Toxoplasma gondii Effect of Rutin in BV2 Cells and Its Mechanism

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