乙酸钠对油酸诱导的BRL-3A细胞脂肪变性的影响及机制分析

doi:10.11843/j.issn.0366-6964.2022.12.030

Abstract

Abstract: Sodium acetate at different concentrations (2, 4, 8 mmol·L^-1) was added to oleic acid-induced steatosis model of normal rat liver cells (BRL-3A). To investigate the regulation mechanism of steatosis cells model lipid metabolism and repair of cell damage, the following experiments were conducted: 1) BRL-3A cells were stimulated with oleic acid at different concentrations (0, 0.03, 0.06, 0.12, 0.24, 0.48 mmol·L^-1) for 24 h. The cell relative activity, total lipid droplet area, triglyceride (TG) content, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were detected to establish cell steatosis model. 2) BRL-3A cells were added with different concentrations of sodium acetate, and the apoptosis rate was detected by flow cytometry. 3)BRL-3A cells were incubated with different concentrations of sodium acetate and 0.12 mmol·L^-1 oleic acid. The experiment was divided into 4 groups, including oleic acid treatment group, 2 mmol·L^-1 sodium acetate + oleic acid treatment group, 4 mmol·L^-1 sodium acetate + oleic acid treatment group and 8 mmol·L^-1 sodium acetate + oleic acid treatment group. Lipid droplets and TG content, AST and ALT activities, AMPK signaling pathway proteins and key genes of lipid metabolism were detected. Results: 1) BRL-3A cells were treated with 0.12 mmol·L^-1 oleic acid for 24 h, and the steatosis model of BRL-3A cells was established successfully. 2)Different concentrations of sodium acetate had no effect on the apoptosis rate of BRL-3A cells. 3)Compared with oleic acid treatment group, the total lipid drop area, lipid drop number per mm², TG content, AST and ALT activities of cell steatosis model treated with 8 mmol·L^-1 sodium acetate were significantly decreased (P<0.05) or extremely significantly decreased (P<0.01). The expression level of P-AMPK was significantly (P<0.05) or extremely significantly increased (P<0.01). The mRNA expression levels of lipid anabolism-related genes ACC, FAS and SCD-1 all decreased to a certain extent; the mRNA expression levels of lipid catabolism-related genes CPT-1, CPT-2 and ACO all increased to a certain extent. This study showed that sodium acetate activates lipid catabolism through the AMPK pathway, attenuated the lipid accumulation in hepatocytes, and alleviated the oleic acid-induced damage of BRL-3A cell steatosis model.

Key words: BRL-3A cells, sodium acetate, steatosis cell model, lipid metabolism

CLC Number:

S856.5

LI Lin, GONG Binbin, WANG Guangli, ZHAO Mei, ZHANG Yuanshu. Effect of Sodium Acetate on Oleic Acid-induced Steatosis in BRL-3A Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4450-4460.

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Effect of Sodium Acetate on Oleic Acid-induced Steatosis in BRL-3A Cells

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