不同鸡种胚胎腿肌差异表达mRNA和lncRNA的筛选及其竞争性调控网络的构建

doi:10.11843/j.issn.0366-6964.2022.12.008

Abstract

Abstract: The objective of this study was to screen differentially expressed mRNAs (DEMs) and lncRNAs (DELs) in skeletal muscle of Tibetan chicken and Daheng broiler in the embryonic stage and construct a lncRNA-miRNA-mRNA regulation network, so as to provide a basis for further investigation of the difference mechanisms in skeletal muscle development between the two breeds. Three embryos of Tibetan chicken and Daheng broiler that has been incubated for 18 days were randomly selected, respectively. The embryonic leg muscle tissues were collected for trans-criptome sequencing. The DEMs and DELs were screened and verified by real-time quantitative PCR (qRT-PCR). The DEMs and DELs were performed by GO and KEGG enrichment analysis. The miRNA that not only as a target for lncRNA but also targeting mRNA were selected to construct a lncRNA-miRNA-mRNA regulatory network. The results showed that a total of 106 DEMs were screened in the two breeds, of which 48 mRNAs were up-regulated and 58 mRNAs were down-regulated. These DEMs significantly enriched in GO terms including skeletal muscle cell differentiation and KEGG pathways including lipid metabolism. Twenty eight DELs were screened, of which 10 lncRNAs were up-regulated and 18 lncRNAs were down-regulated. KEGG analysis of the target genes of lncRNAs showed that many target genes significantly enriched in signaling pathways related to lipid metabolisms, such as steroid biosynthesis and fatty acid biosynthesis. The DEMs and DELs associated with skeletal muscle development were screened and lncRNA-miRNA-mRNA competitive regulation network was constructed. The result of qRT-PCR showed that the expression trend of selected DEMs and DELs was consistent with the sequencing result. The DEMs and DELs related to skeletal muscle development of Tibetan chicken and Daheng broiler were screened to construct the lncRNA-miRNA-mRNA regulatory network. The study provided a theoretical basis for revealing the variability of skeletal muscle growth and development in different chicken breeds.

Key words: Tibetan chicken, Daheng broiler, transcriptome sequencing, muscle development, regulatory network

CLC Number:

S831.2

LI Jie, ZHAO Ruipeng, CHEN Chuwen, YANG Chaowu, WU Jinbo, LI Zhixiong. Screening of the Differentially Expressed mRNA and lncRNA and the Construction of Their Competitive Regulatory Network in Embryonic Leg Muscles of Different Chicken Breeds[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4207-4220.

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Screening of the Differentially Expressed mRNA and lncRNA and the Construction of Their Competitive Regulatory Network in Embryonic Leg Muscles of Different Chicken Breeds

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