微囊化沙门菌SP4噬菌体的制备及其缓释特性分析

doi:10.11843/j.issn.0366-6964.2022.07.028

Abstract

Abstract: Microencapsulated SP4 phage was prepared by using cationic etherified starch/sodium alginate/xanthan gum/nano TiO₂/chitosan oligosaccharide as raw materials, to provide biological agents for controlling Salmonella infection. Salmonella SP4 phage was used to prepare microencapsulated SP4 phage by droplet method, and its titer, cationic etherified starch concentration, sodium alginate concentration, xanthan gum concentration, chitosan oligosaccharide concentration, pH stability, thermal stability, simulated gastric juice stability, simulated intestinal juice release behavior and preservation stability were analyzed. The results showed that when the concentration of cationic etherified starch was 2.4%, the concentration of sodium alginate was 2%, the concentration of xanthan gum was 1%, and the concentration of chitosan oligosaccharide was 0.6%, the shape of microencapsulated SP4 phage microspheres was regular, and the encapsulation efficiency was 97.5%. The phage maintained high biological activity in the range of pH 5.0-8.0, temperature 10-30℃, simulated gastric juice pH 2.0 for 0-30 min and pH 3.0 for 0-150 min. The phage was completely released after 4 h in simulated intestinal juice, and the phage titer did not decrease significantly after 6 weeks at 4℃. The results show that microencapsulated Salmonella SP4 phage significantly improves the stability and sustained-release performance of phage, its acid resistance is conducive to resisting the decomposition of gastric acid, and has the potential to be used in biological control, which lays a foundation for the further identification of microencapsulated Salmonella SP4 phage by multi factor test.

Key words: SP4 phage, microencapsulated coating, stability, slow release performance, biological agent

CLC Number:

S854.5

ZHANG Bo, ZHANG Yongying, HAO He, ZHANG Yibo, LV Xingbang, ZHU Shouchuang, ZHU Zhen, SHI Yuxiang. Preparation of Microencapsulated Salmonella SP4 Bacteriophage and the Study on Its Slow-release Performance[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2317-2325.

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Preparation of Microencapsulated Salmonella SP4 Bacteriophage and the Study on Its Slow-release Performance

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