鹅星状病毒的分离鉴定及全基因组序列分析

doi:10.11843/j.issn.0366-6964.2020.11.015

Abstract

Abstract: From October 2017 to May 2019, 67 samples of typical goslings gout were collected from Guangdong, Sichuan, Hebei, Shandong, Anhui, Liaoning provinces and other vast goose-raising areas in China. The results showed that goose astrovirus was detected in all samples and about 94.03% of the samples were infected by two different kinds of goose astrovirus. The pathogenicity of goose astrovirus FLX variant strain (named SCCD) which could stably proliferate in goose embryos and kill 10-day-old goose embryos was more virulent than goose astrovirus FLX strain. The new type of goose astrovirus strain (named SDPD) could't stably proliferate in goose embryos and SPF chicken embryos. The whole genome nucleotides homology of goose astrovirus FLX strain with goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain were 89.7% and 58.1%, the amino acids homology of ORF1b gene were 98.4% and 61.0%, the amino acids homology of ORF2 gene were 81.0% and 42.5%, respectively. The newly isolated goose astrovirus strains were inoculated into 1-day-old healthy goslings, the results showed that although the goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain could cause the death of goslings and the changes of hepatitis, kidney swelling and weight loss, none of the goslings showed typical gout characteristics (urate deposition in the organs of the body), however, 44% of goslings were characterized with typical gout which was consistent with the symptoms of natural disease after coinfected by the two goose astrovirus strains. Therefore, the pathogenic agents of goslings gout may be two different kinds of goose astrovirus, namely the new type of goose astrovirus and the variant of goose astrovirus FLX.

Key words: goslings gout, two kinds of goose astrovirus, isolation and identification, sequence analysis

CLC Number:

ZHANG Yujie, SUN Ning, LIU Dong, YU Jing, LIU Hongxiang, YU Kechao, REN Yanbei, GAO Tianzuo, YANG Shaoyan, GONG Xiao, ZOU Min, ZHANG Yulong, WANG Yuchao, ZHONG Sheng, MA Dong, DU Yuanzhao. Isolation, Identification and Complete Genomic Sequence Analysis of Goose Astrovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2765-2777.

References 32

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Isolation, Identification and Complete Genomic Sequence Analysis of Goose Astrovirus

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