基于转录组测序数据的山羊下丘脑组织繁殖相关信号通路及候选基因的筛选

doi:10.11843/j.issn.0366-6964.2019.07.007

Abstract

Abstract: The aim of this study was to screen the signaling pathways and candidate genes related to goat reproduction of hypothalamus tissue of Henan Huai goats under different illumination conditions by transcriptome sequencing and bioinformatics analysis. The transcriptome sequencing of 8 infecund ewes hypothalamus tissue (4 in each group, one year old) treated with natural light (8 h light:16 h dark) and artificial light (16 h light:8 h dark) were performed by using a second generation high throughput sequencing platform(NGST), and PE150 sequencing strategy. The total number of unigene assembled was compared to the reference genome sequence, differentially expressed genes(DEGs) analysis, Go and KEGG enrichment analysis and candidate gene screening were performed. The relative expression of candidate genes was analyzed by Real-time PCR. The results showed that about 440 million reads were obtained by RNA-Seq, and the average reads number per sample was about 52.49 million. Four hundred and forty-eight differentially expressed genes were obtained by DESeq analysis between natural and artificial illumination groups, which were enriched in 241 signaling pathways in KEGG database, including 5 reproductive-related signaling pathways. Three candidate genes of tachykinin family(TACR1,TACR2 and TACR3) were significantly enriched in the calcium signaling pathway (chx04020). The results of Real-time PCR analysis showed that the sequencing results of the transcriptome were reliable. These results suggest that calcium signaling pathway (chx04020) and TACR1,TACR2 and TACR3 genes may play the important role in goat reproduction.

CLC Number:

S826.2

SHI Huibin, WANG Yuqin, ZHANG Xiaohui, WU Zhibo, YANG Fang. Selection of Related Signaling Pathways and Candidate Genes Related to Hypothalamus Tissue Reproduction in Goats Based on Transcriptome Sequencing Data[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1380-1395.

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Selection of Related Signaling Pathways and Candidate Genes Related to Hypothalamus Tissue Reproduction in Goats Based on Transcriptome Sequencing Data

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