绵羊GnIH和VIP基因在不同繁殖状态下的表达变化

doi:10.11843/j.issn.0366-6964.2019.05.004

Abstract

Abstract:

The aim of this study was to further elucidate the roles of GnIH and VIP genes in sheep seasonal estrus and reproductive regulation. Adult Sunite sheep (SNT) under short photoperiod and long photoperiod and Small Tail Han sheep (STH) at follicular stage and luteal stage were selected, meanwhile SNT at 21th day of short photoperiod and 3rd, 15th, 21th, 42th, 49th days of long photoperiod were selected, respectively. Gonadal axis tissue samples including hypothalamus, pituitary, pineal body, ovary, oviduct, uterine body were collected in 3 sheep for each reproductive stage. The qPCR method was used to analyze the expression characteristics of GnIH and VIP genes. The results showed that GnIH and VIP genes were expressed in the above 6 tissues, and had a high expression level in hypothalamus tissue of two breeds (SNT and STH). Expression level of two genes under long photoperiod (LP) was extremely significantly higher than that under short photoperiod (SP) in hypothalamus of SNT (P<0.01). Expression level of VIP gene was significantly higher at luteal stage than that at follicular stage (P<0.05), and expression level of GnIH gene was slightly higher at luteal stage than that at follicular stage but the difference was not significant (P>0.05) in hypothalamus of STH. When SP turned into LP, expression levels of two genes in hypothalamus of SNT rose and showed a transient peak at LP 3rd day, then the expression decreased. Quantitative expression characteristics of GnIH and VIP genes in gonadal axis tissues of adult sheep were revealed in this study. Additionally, expression pattern of the 2 genes in hypothalamus under different photoperiods and different reproduction stages further implied that GnIH and VIP genes was involved in the regulation of sheep seasonal estrus and transition of estrous stages. When SP turns into LP, the 2 genes play pivotal role in the first 3 days under LP.

CLC Number:

S826.2

LI Chunyan, LIU Qiuyue, HU Wenping, WANG Xiangyu, MA Lin, ZHANG Xiaosheng, ZHANG Jinlong, DI Ran, CHU Mingxing. Expression Pattern of GnIH and VIP Genes in Different Reproductive States of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 930-938.

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Expression Pattern of GnIH and VIP Genes in Different Reproductive States of Sheep

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