桦木酸对环磷酰胺致小鼠肠道氧化损伤的影响

doi:10.11843/j.issn.0366-6964.2018.04.020

Abstract

Abstract:

The experiment was conducted to evaluate the effect of betulinic acid (BA) on intestinal oxidative damage induced by cyclophosphamide (Cy) in mice. Male Kunming mice were randomly divided into 5 groups:control group (NC), Cy group, 0.5 mg·kg^-1 BA group, 5.0 mg·kg^-1 BA group and 50.0 mg·kg^-1 BA group. The control and Cy group were administered orally with 1% starch and the other groups were administered orally with different doses of BA (0.5, 5.0, and 50.0 mg·kg^-1) daily for 14 days. The model of intestinal oxidative damage was set up in mice induced by Cy at the dosage of 50 mg·kg^-1 bw for 2 days after last administration of BA. The liver, spleen and thymus index of mice were measured, the activity of secretory immunoglobulin a (SIgA) in intestinal mucosa and the levels of the superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) in intestine and serum were determined. BA pretreatment relieved atrophy of thymus, spleen and liver, inhibited the decline of SIgA activity in intestinal mucosa induced by Cy. Pretreatment with BA reduced the contents of GSH and MDA in small intestine and serum induced by Cy, but had no effect on SOD activity. BA could improve intestinal lipid peroxidation and increase the secretion of SIgA in the intestine mucosa and shows preventive protection of Intestinal oxidative damage induced by Cy.

CLC Number:

S856.4

WANG Xi-hong, ZHU Li-juan, YI Xiang-lian, ZHAO Jing, XIANG Si-ting, TAN Zhu-liang, YUAN Zhi-hang, YI Jin-e. Effects of Betulinic Acid on Intestinal Mucosal Damage Induced by Cyclophosphamide in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 818-824.

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References

[1] 孙怡燕. 浅谈肠道的重要功能[J]. 医药前沿, 2012(22):283.
SUN Y Y. Talking about the important function of gut[J]. Frontiers of Medicine, 2012(22):283. (in Chinese)
[2] 李维, 孙开济, 孙玉丽, 等. 乳酸菌缓解肠道氧化应激研究进展[J]. 动物营养学报, 2016, 28(1):9-14.
LI W, SUN K J, SUN Y L, et al. Research progress in relieve effect of lactobacillus in intestinal oxidative stress[J]. Chinese Journal of Animal Nutrition, 2016, 28(1):9-14. (in Chinese)
[3] 杨雪芬, 周桂莲. 氧化应激对鸡肠道健康的影响研究[J]. 中国家禽, 2011, 33(24):7-11.
YANG X F, ZHOU G L. Study of oxidative stress on intestinal health effects[J]. China Poultry, 2011, 33(24):7-11. (in Chinese)
[4] 刘妙娜, 王腾, 李巍, 等. 桦木酸抗肝纤维化作用的实验研究[J]. 华中科技大学学报:医学版, 2015, 44(6):682-685, 690.
LIU M N, WANG T, LI W, et al. Effect of betulinic acid on carbon tetrachloride-induced liver fibrosis in rats[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2015, 44(6):682-685, 690. (in Chinese)
[5] 易金娥, 邬静, 文利新, 等. 桦木酸的药理作用研究进展[J]. 中草药, 2014, 45(14):2118-2124.
YI J E, WU J, WEN L X, et al. Research progress on pharmacological activities of betulinic acid[J]. Chinese Traditional and Herbal Drugs, 2014, 45(14):2118-2124. (in Chinese)
[6] CICHEWICZ R H, KOUZI S A. Chemistry, biological activity, and chemotherapeutic potential of betulinic acid for the prevention and treatment of cancer and HIV infection[J]. Med Res Rev, 2004, 24(1):90-114.
[7] ALAKURTTI S, MÄKELÄ T, KOSKIMIES, et al. Pharmacological properties of the ubiquitous natural product betulin[J]. Eur J Pharm Sci, 2006, 29(1):1-13.
[8] YI J E, OBMINSKA-MRUKOWICZ B, YUAN L Y, et al. Immunomodulatory effects of betulinic acid from the bark of white birch on mice[J]. J Vet Med Sci, 2010, 11(4):305-313.
[9] 易金娥, 袁莉芸, 文利新, 等. 桦木酸对小鼠淋巴细胞及巨噬细胞的影响分析[J]. 畜牧兽医学报, 2011, 42(1):124-130.
YI J E, YUAN L Y, WEN L X, et al. Effects of betulinic acid on lymphocyte and macrophage in mice[J]. Acta Veterinaria et Zootechnica Sinica, 2011, 42(1):124-130. (in Chinese)
[10] 易金娥, OBMINSKA-MRUKOWICZ B, 杜金艳, 等. 桦木酸对巨噬细胞免疫功能和抗氧化作用的研究[J]. 营养学报, 2010, 32(3):281-285.
YI J E, OBMINSKA-MRUKOWICZ B, DU J Y, et al. Effects of betulinic acid on immune function and antioxidant activity of mouse peritoneal macrophages[J]. Acta Nutrimenta Sinica, 2010, 32(3):281-285. (in Chinese)
[11] 易金娥, 屠迪, 邬静, 等. 桦木酸对小鼠免疫器官抗氧化能力的影响[J]. 动物营养学报, 2012, 24(4):786-790.
YI J E, TU D, WU J, et al. Effects of betulinic acid on antioxidant ability of immune organs in mice[J]. Acta Zoonutrimenta Sinica, 2012, 24(4):786-790. (in Chinese)
[12] ZUCO V, SUPINO R, RIGHETTI S C, et al. Selective cytotoxicity of betulinic acid on tumor cell lines, but not on normal cells[J]. Cancer Lett, 2002, 175(1):17-25.
[13] 夏伟, 朱若岑, 蒋维维, 等. 桦木酸对小鼠酒精性肝损伤的保护作用[J]. 营养学报, 2015, 37(1):68-72.
XIA W, ZHU R C, JIANG W W, et al. Protection of betulinic acid against liver injury induced by alcohol in mice[J]. Acta Nutrimenta Sinica, 2015, 37(1):68-72. (in Chinese)
[14] YI J E, ZHU R C, WU J P, et al. In vivo protective effect of betulinic acid on dexamethasone induced thymocyte apoptosis by reducing oxidative stress[J]. Pharmacol Rep, 2016, 68(1):95-100.
[15] YI J E, ZHU R C, WU J P, et al. Ameliorative effect of betulinic acid on oxidative damage and apoptosis in the splenocytes of dexamethasone treated mice[J]. Int Immunopharmacol, 2015, 27(1):85-94.
[16] 易金娥, 文立新, 袁莉芸, 等. 白桦树中桦木醇的提取与桦木酸合成研究[J]. 湖南农业大学学报:自然科学版, 2010, 36(3):574-580.
YI J E, WEN L X, YUAN L Y, et al. Study on extraction of betulin from the white birch and synthesis of betulinic acid[J]. Journal of Hunan Agricultural University:Natural Sciences, 2010, 36(3):574-580. (in Chinese)
[17] 张志远, 王海莉, 苗明三. 扶正中药复方对环磷酰胺所致免疫功能低下小鼠免疫器官和肠黏膜CD3⁺、CD4⁺、CD8⁺的影响[J]. 中医研究, 2008, 21(12):17-20.
ZHANG Z Y, WANG H L, MIAO M S. Effect of Fuzheng Herbal Compound on CD3⁺, CD4⁺ and CD8⁺ of immune organs and intestinal mucosa in immunodeficient mice induced by cyclophosphamide[J]. Traditional Chinese Medicinal Research, 2008, 21(12):17-20. (in Chinese)
[18] 吴皓, 林洪生, 裴迎霞, 等. 人参皂甙Rg3对荷瘤及环磷酰胺化疗小鼠黏膜免疫力影响[J]. 中国肿瘤, 2006, 15(6):369-371.
WU H, LIN H S, PEI Y X, et al. Effect of ginsenosid Rg3 on the mucosal immunity in tumor-bearing mice treated with cyclophosphamide[J]. Bulletin of Chinese Cancer, 2006, 15(6):369-371. (in Chinese)
[19] 严胜泽, 马玉芳, 廖吕燕, 等. 太子参多糖对环磷酰胺所致肠道黏膜损伤小鼠SIgA、IL-2、IL-6含量的影响[J]. 中国畜牧兽医, 2015, 42(5):1187-1192.
YAN S Z, MA Y F, LIAO L Y, et al. Effect of pseudostellaria polysaccharides on SIgA, IL-2 and IL-6 contents of intestinal mucosal injured mice caused by cyclophosphamide[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(5):1187-1192. (in Chinese)
[20] 曹露. 鱿鱼墨多糖对环磷酰胺所致小鼠肠道黏膜上皮细胞损伤的保护作用研究[D]. 青岛:中国海洋大学, 2013.
CAO L. Protective Effects of polysaccharides from the ink of ommastrephes bartrami on cyclophosvnamide-induced mucosa epithelial cell's injury in mice small intestine[D]. Qingdao:Ocean University of China, 2013. (in Chinese)
[21] 郭登方. 脾切除自体移植脾组织血管、神经再生及对脾功能恢复调控意义的研究[D]. 福州:福建医科大学, 2015.
GUO D F. Study on the regeneration of blood vessels, nerves and their regulation for immune function in auto-transplanted spleen[D]. Fuzhou:Fujian Medical University, 2015. (in Chinese)
[22] 宋金星, 薛协超, 党冰怡, 等. 0~28日龄SPF仔大鼠免疫功能变化规律的研究[J]. 黑龙江畜牧兽医, 2017(9):245-247.
SONG J X, XUE X C, DANG B Y, et al. Study on the change of immune function in 0~28-day-old SPF rats[J]. Heilongjiang Animal Science and Veterinary Medicine, 2017(9):245-247. (in Chinese)
[23] SHETH K, BANKEY P. The liver as an immune organ[J]. Curr Opin Crit Care, 2001, 7(2):99-104.
[24] TRIGER D R. The liver as an immunological organ[J]. Gastroenterology, 1976, 71(1):162-164.
[25] 闫蕾, 赵彩彦. 肝脏-一个新的免疫器官[J]. 国际内科学杂志, 2009, 36(4):206-210.
YAN L, ZHAO C Y. Liver-a new immune organ[J]. International Journal of Internal Medicine, 2009, 36(4):206-210. (in Chinese)
[26] 苗明三, 顾丽亚, 方晓艳, 等. 当归补血汤多糖对气血双虚大鼠胸腺、脾脏的影响[J]. 中国组织工程研究, 2005, 9(3):162-163.
MIAO M S, GU L Y, FANG X Y, et al. Effect of Danggui Buxue Tang polysaccharide on thymus and spleen of rats with the deficiencies of both Qi and blood[J]. Chinese Journal of Clinical Rehabilitation, 2005, 9(3):162-163. (in Chinese)
[27] 徐元庆, 史彬林, 郭祎玮, 等. 壳聚糖对断奶仔猪免疫器官及胃肠道发育的影响[J]. 饲料工业, 2013, 34(3):32-35.
XU Y Q, SHI B L, GUO Y W, et al. Effects of chitosan on the development of immune organs and gastrointestinal tracts in weaned piglets[J]. Feed Industry, 2013, 34(3):32-35. (in Chinese)
[28] YI J E, XIA W, WU J P, et al. Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice[J]. J Vet Sci, 2014, 15(1):141-148.
[29] 王定越, 徐盛玉, 宋洁, 等. 分泌性免疫球蛋白A与肠道黏膜免疫的关系及其分泌的营养调控[J]. 动物营养学报, 2012, 24(10):1854-1860.
WANG D Y, XU S Y, SONG J, et al. Secretory immunoglobulin A:Relationship with intestinal mucosal immunity and nutritional regulation of its secretion[J]. Acta Zoonutrimenta Sinica, 2012, 24(10):1854-1860. (in Chinese)
[30] 宝中, 冉多良, 童贻刚. 分泌型免疫球蛋白A的研究进展[J]. 生物技术通讯, 2009, 20(2):263-265.
BAO Z, RAN D L, TONG Y G. Advances in research on secretory immunoglobulin A[J]. Letters in Biotechnology, 2009, 20(2):263-265. (in Chinese)
[31] 李伟, 陈庆森. 肠道黏膜免疫屏障及其菌群与机体健康关系的研究进展[J]. 食品科学, 2008, 29(10):649-655.
LI W, CHEN Q S. Research progress of relationship of human health with intestinal mucosal immunity and microflora[J]. Food Science, 2008, 29(10):649-655. (in Chinese)
[32] 谢全喜, 张建梅, 谷巍. 植物乳杆菌对肉鸡生长性能、免疫功能和抗氧化能力的影响[J]. 中国饲料, 2013(14):26-30.
XIE Q X, ZHANG J M, GU W. Effects of lactobacillus plantarum on growth performance, immune function and antioxidant of broilers[J]. China Feed, 2013(14):26-30. (in Chinese)
[33] 李生, 杨勇, 丁百静, 等. 非酒精性脂肪性肝炎大鼠肠道分泌液sIgA的变化[J]. 胃肠病学和肝病学杂志, 2011, 20(2):124-127.
LI S, YANG Y, DING B J, et al. Changes of sIgA of intestinal mucosal fluid in the progress of non-alcoholic steatohepatitis in rats[J]. Chinese Journal of Gastroenterology and Hepatology, 2011, 20(2):124-127. (in Chinese)
[34] 朱若岑, 夏伟, 谭柱良, 等. 桦木酸对Dex致小鼠氧化损伤的保护作用[J]. 中国兽医学报, 2016, 36(2):305-309.
ZHU R C, XIA W, TAN Z L, et al. Protective effect of betulinic acid on oxidative damage induced by dexamethasone in mice[J]. Chinese Journal of Veterinary Science, 2016, 36(2):305-309. (in Chinese)
[35] 帅学宏, 胡庭俊, 陈炅然, 等. 鬼臼多糖对小鼠免疫功能和抗氧化能力的影响[J]. 畜牧兽医学报, 2008, 39(7):1000-1004.
SHUAI X H, HU T J, CHEN J R, et al. Effects of podophyllum emodi polysaccharide on immune function and antioxidant capability in mice[J]. Acta Veterinaria et Zootechnica Sinica, 2008, 39(7):1000-1004. (in Chinese)
[36] 李晓玲. 香烟提取物对肺间质纤维化的影响机制以及还原型谷胱甘肽的干预作用[D]. 石家庄:河北医科大学, 2014.
LI X L. The mechanism of the cigarette smoke extract effects on pulmonary fibrosis and the intervention effect of the reduced glutathione[D]. Shijiazhuang:Hebei Medical University, 2014. (in Chinese)
[37] 崔筱琳, 王凤山. 谷胱甘肽防治疾病的研究进展[J]. 中国现代应用药学, 2017, 34(4):631-636.
CUI X L, WANG F S. Research progress of glutathione in the prevention and treatment of diseases[J]. Chinese Journal of Modern Applied Pharmacy, 2017, 34(4):631-636. (in Chinese)

Effects of Betulinic Acid on Intestinal Mucosal Damage Induced by Cyclophosphamide in Mice

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