miR-186-5p对绵羊黑色素细胞迁移增殖的影响

doi:10.11843/j.issn.0366-6964.2017.11.007

Abstract

Abstract:

The aim of this study was to explore influence of miR-186-5p on migration and proliferation of sheep melanocytes. The expression of miR-186-5p in different color skin of sheep were determined by Quantitative real-time PCR; The over-expression vectors of miR-186-5p were transfected into sheep melanocytes, and then, the expression of miR-186-5p was analyzed using Quantitative real-time PCR; The melanin content were detected by spectrophotometer; The capacity of migration and proliferation were analyzed by wound-healing assay. The results showed that the expression of miR-186-5p was significantly higher in white skin than that in black skin (P<0.05); In transfected melanocytes with over-expression of miR-186-5p, the expression of miR-186-5p was significantly increased (P<0.01), the level of melanin content was significantly reduced(P<0.05),the capacity of migration and proliferation of melanocytes also reduced. In summary, miR-186-5p was associated with the formation of melanin in sheep skin, in sheep melanocytes, overexpression of miR-186-5p would reduce the melanin production and suppress the migration and proliferation of melanocytes.

CLC Number:

S826.2

XU Dong-mei, ZHAO Yu-jun, DU Bin, ZHANG Ya-ling, ZHU Zhi-wei, XIE Jian-shan, FAN Rui-wen, YU Xiu-ju, DONG Chang-sheng. The Influences of miR-186-5p on Migration and Proliferation of Sheep Melanocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2068-2075.

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The Influences of miR-186-5p on Migration and Proliferation of Sheep Melanocytes

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