死亡受体通路在黄曲霉毒素B1致雏鸡胸腺细胞凋亡中的作用

doi:10.11843/j.issn.0366-6964.2017.09.021

Abstract

Abstract:

This experiment was conducted to investigate the regulation mechanism of death receptor pathway involved in the aflatoxins (AF) B₁-induced thymocytes apoptosis. Ninety healthy chickens were randomly divided into two groups, and fed on control diet and AFB₁ containing diet (formulated by adding 0.6 mg·kg^-1 AFB₁ into the control diet) respectively for 21 days. Thymus was sampled for detecting the relative weight, T-cell subsets, percentage of apoptotic cells and relative mRNA expression of genes in the death receptor pathway at 7, 14 and 21 days of age. Our results showed that in the AFB₁ group, the relative weight of thymus was decreased; the percentages of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺ T-cells were decreased, and the percentage of apoptotic thymocytes was increased, when compared with those in the control group. Also, the relative mRNA transcription of RIP1, Caspase-8, Caspase-9, Caspase-3, Fas, FasL, ASK1, IKIP and JNK were up-regulated, and those of Bcl-2, NF-κB1 and Bid were down-regulated. It was speculated that the mechanisms of the excessive apoptosis of thymocytes could be related to the following three downstream death receptor pathways:①AFB₁ could directly induce the apoptosis through the Fas-FasL-FADD-Caspase-8-Caspase-3 pathway; ②Fas could take part in the excessive apoptosis through Fas-ASK1-JNK-Bcl-2 pathway which was mediated via mitochondria; ③The constitutive activation of the TNF-α-RIP1-IKIP-NF-κB1-caspase-3 could be a pathway contributing to the apoptosis of thymocytes induced by AFB₁.

CLC Number:

S856.9

PENG Xi, LIANG Na, FANG Jing, WU Bang-yuan. Study on Regulation Mechanism of Death Receptor Pathway Related to Up-regulated Apoptosis of Thymocytes in Broilers after AFB₁ Exposure[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1744-1752.

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Study on Regulation Mechanism of Death Receptor Pathway Related to Up-regulated Apoptosis of Thymocytes in Broilers after AFB₁ Exposure

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Study on Regulation Mechanism of Death Receptor Pathway Related to Up-regulated Apoptosis of Thymocytes in Broilers after AFB1 Exposure

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Study on Regulation Mechanism of Death Receptor Pathway Related to Up-regulated Apoptosis of Thymocytes in Broilers after AFB₁ Exposure