胎次对奶山羊胎盘提取物抗氧化活性影响

doi:10.11843/j.issn.0366-6964.2017.08.007

Abstract

Abstract:

The objective of this work was to explore the impact of parities on antioxidant activities of extractions from dairy goat placenta, which could provide technical foundation to the application of placenta. The scavenging rate of DPPH radical and hydroxyl radical were taken as the evaluate indices of antioxidant peptides activity, the water-soluble protein content in the extract was also taken into account. The changes of placenta extract indices for different parities were compared and the attenuation of indicators with the change of storage conditions was investigated. The results showed that:1) The polypeptides contents of 1^st, 2^nd, 3^rd parities in placenta were (0.201±0.03), (0.270±0.02) and (0.193±0.02) mg·mg^-1 dry matter, respectively. The DPPH radical scavenging rate of 1^st, 2^nd, 3^rd parities were 32.75%, 53.20% and 37.97%.The scavenging rate of hydroxyl radicals of these 3 parities were 35.45%, 51.94% and 39.35%. The water-soluble protein content were 16.63%, 23.87% and 18.60%, respectively. 2) Compared with the ambient temperature, the decay rate was slower under the condition of 4℃, there was significant difference among parities under different conditions (P<0.05). 3) The preservation rate of DPPH radical scavenging of 2^nd parities at 4℃ was 78.85%.The preservation rate of hydroxyl radicals scavenging activity of 2^nd parities was 71.93%. The preservation rate of the water-soluble protein content was 77.36%. In conclusion, it is demonstrated that the 2^nd parities was the most prominent on antioxidant activity, dacay rate and WSP content and 4℃ was the ideal storage temperature for the preservation of activities.

CLC Number:

S826.2

GAO Shan-shan, GE Wu-peng, PEI Jian-fei, WANG Xi-ning, LI Xiao-peng, LIANG Xiu-zhen, WU Xiao-yong. Effect of Different Parities on the Antioxidant Activity of Dairy Goat Placenta Extract[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1436-1445.

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References

[1] 王顺新. 酶法水解牛胎盘下脚料的工艺研究[J]. 食品安全导刊, 2016(9):93-95.
WANG S X. Study on the technology of enzymatic hydrolysis of bovine placenta[J]. China Food Safety, 2016(9):93-95. (in Chinese)
[2] 刘旺旺, 侯银臣, 王皓, 等. 不同菌种发酵羊胎盘残留物制备活性肽研究比较[J]. 食品工业, 2015, 36(8):149-153.
LIU W W, HOU Y C, WANG H, et al. Comparative study on activities of placenta peptide produced by different microbiological strains ferment goat placenta residue[J]. Food Industry, 2015, 36(8):149-153. (in Chinese)
[3] HARNEDY P A, FITZGERALD R J. Bioactive peptides from marine processing waste and shellfish:A review[J]. J Funct Foods, 2012, 4(1):6-24.
[4] SARMADI B H, ISMAIL A. Antioxidative peptides from food proteins:A review[J]. Peptides, 2010, 31(10):1949-1956.
[5] KATE R, EL-DIN B A, MICHELLE M, et al. Towards generation of bioactive peptides from meat industry waste proteins:generation of peptides using commercial microbial proteases[J]. Food Chem, 2016, 208:42-50.
[6] AHMED A S, EL-BASSIONY T, ELMALT L M, et al. Identification of potent antioxidant bioactive peptides from goat milk proteins[J]. Food Res Int, 2015, 74:80-88.
[7] EBNER J, ARSLAN A A, FEDOROVA M, et al. Peptide profiling of bovine kefir reveals 236 unique peptides released from caseins during its production by starter culture or kefir grains[J]. J Proteomics, 2015, 117:41-57.
[8] 韩英东, 杨亮, 潘晓花, 等. 胎次和泌乳阶段对荷斯坦奶牛血液乳成分前体物及泌乳相关激素含量的影响[J]. 动物营养学报, 2015, 27(4):1135-1144.
HAN Y D, YANG L, PAN X H, et al. Effects of parity and stage of lactation on milk component precursor and lactation related hormone contents in blood of Holstein dairy cows[J]. Chinese Journal of Animal Nutrition, 2015, 27(4):1135-1144. (in Chinese)
[9] 吴双军, 尚娇, 尚海彬, 等. 不同年龄、胎次和泌乳月对奶牛乳脂率的影响[J]. 安徽农业科学, 2016, 44(14):125-126.
WU S J, SHANG J, SHANG H B, et al. Effects of dairy cow age, fetal times and lactation month on the butter-fat percentage[J]. Journal of Anhui Agricultural Sciences, 2016, 44(14):125-126. (in Chinese)
[10] 金亚东, 李艳艳, 徐晓锋. 胎次和泌乳阶段对奶牛体细胞数和乳损失量的影响[J]. 乳业科学与技术, 2016, 39(3):9-13.
JIN Y D, LI Y Y, XU X F. Effects of parity and lactation stage on somatic cell score and milk production loss in dairy cows[J]. Journal of Dairy Science and Technology, 2016, 39(3):9-13. (in Chinese)
[11] 刘志安, 刘武军, 孟军, 等. 不同胎次放牧伊犁马产乳性能及乳品质相关性研究[J]. 新疆农业科学, 2015, 52(2):360-365.
LIU Z A, LIU W J, MENG J, et al. The correlation study of mare's milk performance and milk quality of different parity grazing Yili horses[J]. Xinjiang Agricultural Sciences, 2015, 52(2):360-365. (in Chinese)
[12] 李双红, 刘永峰, 韩裕睿, 等. 不同胎次奶牛乳中乳蛋白含量的近红外光谱定量分析[J]. 食品工业科技, 2014, 35(4):60-65.
LI S H, LIU Y F, HAN Y R, et al. Quantitative analysis of milk protein content in different parities cows based on near infrared spectroscopy[J]. Science and Technology of Food Industry, 2014, 35(4):60-65. (in Chinese)
[13] 孙雨辰. 牛乳的酶凝固特性及其影响因素研究[D]. 长春:吉林农业大学, 2015.
SUN Y C. Study on enzymatic coagulation properties of bovine milk and the influencing factors[D]. Changchun:Jilin Agricultural University, 2015. (in Chinese)
[14] VASSEUR E, GIBBONS J, RUSHEN J, et al. Development and implementation of a training program to ensure high repeatability of body condition scoring of dairy cows[J]. J Dairy Sci, 2013, 96(7):4725-4737.
[15] 邵尊亚, 雷赵民, 宫旭胤, 等. ‘河西绒山羊’乳营养成分及理化性质研究[J]. 甘肃农业大学学报, 2014, 49(2):25-28.
SHAO Z Y, LEI Z M, GONG X Y, et al. Milk nutritional components and physic-chemical properties of ‘Hexi cashmare goat’[J]. Journal of Gansu Agricultural University, 2014, 49(2):25-28. (in Chinese)
[16] 周洁静, 侯银臣, 刘旺旺, 等. 采用不同蛋白酶制备羊胎盘抗氧化肽的研究[J]. 食品工业科技, 2015, 36(5):156-161.
ZHOU J J, HOU Y C, LIU W W, et al. Study on producting antioxidant peptide from goat placenta by different proteases[J]. Science and Technology of Food Industry, 2015, 36(5):156-161. (in Chinese)
[17] ARZENI C, MARTíNEZ K, ZEMA P, et al. Comparative study of high intensity ultrasound effects on food proteins functionality[J]. J Food Eng, 2012, 108(3):463-472.
[18] 焦艳丽, 杜冰, 郭晓明, 等. 超声提取羊胎盘水溶性活性蛋白[J]. 食品与发酵工业, 2011, 37(11):224-228.
JIAO Y L, DU B, GUO X M, et al. Study on ultrasonic extraction of activity protein from sheep placenta[J]. Food and Fermentation Industries, 2011, 37(11):224-228. (in Chinese)
[19] 鲁伟, 任国谱, 宋俊梅. 蛋白水解液中多肽含量的测定方法[J]. 食品科学, 2005, 26(7):169-171.
LU W, REN G P, SONG J M. Determination of content of peptides in protein hydrolysates[J]. Food Science, 2005, 26(7):169-171. (in Chinese)
[20] WU H C, CHEN H M, SHIAU C Y. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus)[J]. Food Res Int, 2003, 36(9-10):949-957.
[21] 罗群. 考马斯亮蓝法快速测定菜籽粕中可溶性蛋白质的含量[J]. 成都大学学报:自然科学版, 2014, 33(2):125-126, 129.
LUO Q. Rapid determination of soluble protein content in rapeseed meal by coomassie brilliant blue method[J]. Journal of Chengdu University:Natural Science Edition, 2014, 33(2):125-126, 129. (in Chinese)
[22] DE FALCO S. The discovery of placenta growth factor and its biological activity[J]. Exp Mol Med, 2012, 44(1):1-9.
[23] FANG X P, XIA W S, SHENG Q H, et al. Purification and characterization of an immunomodulatory peptide from bovine placenta water-soluble extract[J]. Prep Biochem Biotechnol, 2007, 37(3):173-184.
[24] HAN N R, KIM K Y, KIM M J, et al. Porcine placenta mitigates protein-energy malnutrition-induced fatigue[J]. Nutrition, 2013, 29(11-12):1381-1387.
[25] 刘旺旺, 侯银臣, 程永霞, 等. 羊胎盘多肽的制备及其清除自由基能力的研究[J]. 中国酿造, 2014, 33(9):89-93.
LIU W W, HOU Y C, CHENG Y X, et al. Polypeptide preparation of sheep placenta and its free radicals scavenging activity[J]. China Brewing, 2014, 33(9):89-93. (in Chinese)
[26] 马兴亮. 奶山羊胎盘肽的分离提纯及其对小白鼠免疫功能的影响[D]. 泰安:山东农业大学, 2008.
MA X L. Isolation of dairy goat placental peptide and effect on immune function of mice[D]. Tai'an:Shandong Agricultural University, 2008. (in Chinese)
[27] 魏鹏飞, 张淑君, 滑国华. 不同养殖模式下产犊季节、泌乳季节及胎次对荷斯坦牛产奶量及乳品质的影响[J]. 中国奶牛, 2017(1):21-25.
WEI P F, ZHANG S J, HUA G H. Effects of calving season, lactation season and parity on milk yield and quality in dairy cows[J]. China Dairy Cattle, 2017(1):21-25. (in Chinese)
[28] 王璐菊, 郭全奎, 张勇. 产犊季节及胎次对甘肃会宁地区荷斯坦牛产奶量的影响[J]. 中国牛业科学, 2015, 41(3):42-46.
WANG L J, GUO Q K, ZHANG Y. Effects of different calving seasons and parities on milk production for Holstein cows in the Huining region of Gansu[J]. China Cattle Science, 2015, 41(3):42-46. (in Chinese)
[29] WANG B, LI Z R, CHI C F, et al. Preparation and evaluation of antioxidant peptides from ethanol-soluble proteins hydrolysate of Sphyrna lewini muscle[J]. Peptides, 2012, 36(2):240-250.
[30] ZHU L J, CHEN J, TANG X Y, et al. Reducing, radical scavenging, and chelation properties of in vitro digests of alcalase-treated zein hydrolysate[J]. J Agric Food Chem, 2008, 56(8):2714-2721.
[31] LUO H Y, WANG B, LI Z R, et al. Preparation and evaluation of antioxidant peptide from papain hydrolysate of Sphyrna lewini muscle protein[J]. LWT Food Sci Technol, 2013, 51(1):281-288.
[32] TANG W L, ZHANG M, FANG Z X. Optimization of ultrasound-assisted-extraction of porcine placenta water-soluble proteins and evaluation of the antioxidant activity[J]. J Food Sci Technol, 2015, 52(7):4042-4053.
[33] ZOU T B, HE T P, LI H B, et al. The structure-activity relationship of the antioxidant peptides from natural proteins[J]. Molecules, 2016, 21(1):72.
[34] HE R, JU X R, YUAN J, et al. Antioxidant activities of rapeseed peptides produced by solid state fermentation[J]. Food Res Int, 2012, 49(1):432-438.
[35] 徐雪峰, 李桂娟, 闫浩, 等. 赤灵芝多糖分离纯化及体外抗氧化性研究[J]. 食品与机械, 2017, 33(1):144-147.
XU X F, LI G J, YAN H, et al. Study on isolation and antioxidant activity of the polysaccharide from Ganoderma lucidum[J]. Food & Machinery, 2017, 33(1):144-147. (in Chinese)
[36] 唐文林. 猪胎盘综合利用工艺与应用研究[D]. 无锡:江南大学, 2014.
TANG W L. The comprehensive utilization technology and application of porcine placenta[D]. Wuxi:Jiangnan University, 2014. (in Chinese)
[37] 戴五洲, 郑云林. 胎次及分娩季节对母猪繁殖性能的影响[J]. 黑龙江畜牧兽医, 2016(6):81-82.
DAI W Z, ZHENG Y L. Effects of pregnancy and delivery season on reproductive performance of sows[J]. Heilongjiang Animal Science and Veterinary Medicine, 2016(6):81-82. (in Chinese)
[38] COZZI G, RAVAROTTO L, GOTTARDO F, et al. Short communication:Reference values for blood parameters in Holstein dairy cows:Effects of parity, stage of lactation, and season of production[J]. J Dairy Sci, 2011, 94(8):3895-3901.
[39] 曹迎春, 陈铁桥. 繁殖母猪胎次与生产性能及疾病的关系[J]. 畜牧与兽医, 2003, 35(3):25-26.
CAO Y C, CHEN T Q. The relationship between sow parity and production performance and disease[J]. Animal Husbandry & Veterinary Medicine, 2003, 35(3):25-26. (in Chinese)

Effect of Different Parities on the Antioxidant Activity of Dairy Goat Placenta Extract

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