ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (6): 1293-1298.doi: 10.11843/j.issn.0366-6964.2016.06.028

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Toxonomic Study of Some Babesia spp.Based on COⅠ Gene in China

GOU Hui-tian1,XUE Hui-wen1,YIN Hong2,SUN Xiao-lin 1* ,LUO Jian-xun 2*   

  1. (1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Science,Lanzhou 730046,China)
  • Received:2015-11-16 Online:2016-06-23 Published:2016-06-23

Abstract:

In order to clarify the taxonomic status of Babesia spp,the COⅠgene were sequenced from 11 isolates of 7 species reported in China;The phylogenetic tree were constructed using COⅠand 18S rRNA sequences downloaded from GenBank and this study,and also the two phylogenetic trees were compared.As result shown,the length of COⅠgene was 935-999 bp,and the COⅠgene had more variation and informative sites than 18S rRNA.The taxonomic status of bovis Babesia spp.based on COⅠand 18S rRNA shared high similarity.However,there were also some differences from two phylogenetic trees.Such as,the COⅠgene can differentiate Babesia spp and Theileria spp clearly.To 18S rRNA,it was more difficult.The taxonomic status based on COⅠgene of Babesia sp.from Xinjiang was more reasonable.The information of two phylogenetic tree suggested that the Babesia motasi isolated from different region may represent different subspecies.This study supported the candidate gene of molecular taxonomy of Babesia spp.

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