ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (8): 1720-1726.doi: doi: 10.11843/j.issn.0366-6964.2016.08.025

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Analysis of the Serum Concentrations and mRNA Expression Levels of GnRH and GnIH in Geese during Different Reproductive Periods

ZHANG Ke-shan 1,2,HU Yan-jingke,HAN Xiao-zhe 3,GAO Guang-liang 1,2,ZHONG Hang 1,2,WANG Qi-gui 1,2*   

  1. (1.Chongqing Academy of Animal Science,Chongqing 402460,China;
    2.Chongqing Engineering Research Center of Goose Genetic Improvement,Chongqing 402460,China;3.Southwest University,Chongqing 402460,China)
  • Received:2016-02-25 Online:2016-08-23 Published:2016-08-23

Abstract:

To investigate the role of gonadotropin-releasing hormone and gonadotropin-inhibitory hormone genes (GnRH and GnIH,respectively)during the reproductive process of geese,the Sichuan White goose and Xupu goose were selected as experimental animals.We detected the mRNA expression profiles of GnRH and GnIH in the hypothalamus,pituitary and ovary tissues using real-time reverse transcription PCR.Serum concentrations of GnRH and GnIH were respectively measured using radio immunoassay and ELISA during prelaying and laying periods and the period when laying ceased.GnIH and GnRH mRNA were expressed in the hypothalamus,pituitary and ovary tissues.GnRH mRNA expression of the hypothalamus and the ovary were significantly higher in Sichuan White geese than in Xupu geese in both prelaying and laying periods,particularly in the latter.Serum GnRH concentrations were significantly higher or higher in Sichuan White geese (51.13 and 51.10 pg•mL-1) than in Xupu geese(49.52 and 49.94 pg•mL-1) during the laying and cease period,respectively.During the laying period,GnIH mRNA expression of Sichuan White geese was significantly lower than in Xupu geese;however,there were no significant differences inserum GnIH concentrations between the 2 geese during prelaying,laying or cease periods.These results indicate that GnRH is more important than GnIH in regulating laying performance and changing the laying stages which establishes the foundation for understanding the breeding mechanisms of geese.

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