精子动力学分析与其体内、体外受精能力的相关性

doi:doi: 10.11843/j.issn.0366-6964.2016.08.026

Abstract

Abstract:

This study was performed to predict fertility of bull sperm during in vitro fertilization (IVF) and artificial insemination (AI) according to its kinetics parameters.The sex-sorted sperm from 3 Holstein bulls were estimated with computer assisted sperm analysis system (CASA).Moreover，the IVF and AI with those sperm were carried out.The rate of fertilization，embryo cleavage，blastocyst formation and TCN，ICM/TCN of blastocyst obtained by IVF，conception rate during AI were counted and analyzed.Results showed that VAP (102.82 vs 91.55 vs 80.67)，VCL (201.75 vs 180.23 vs 168.58)，ALH (9.33 vs 8.31 vs 7.42) of sperm were significantly different among bull A，B，C (P<0.05).Additionally above 3 parameters were positively correlated with the fertilization rate，cleavage rate of IVF embryos.Sorted sperm from bull A had a significantly higher BCF (20.36 vs 18.05 & 16.23) and LIN (46.06 vs 42.00 & 40.81) than that from bull B，C (P<0.05).Also，the 2 parameters were positively correlated with blastocyst rate of IVF embryos.Vitality (29.00 vs 38.00 & 80.62)，VSL (68.20 vs 36.50 & 79.00) of sperm from bull C were significantly lower than that from bull A and B (P<0.05)，and had a positive correlation with ICM/TCN of blastocysts.However there was no correlation between the kinetics parameters and conception rate in AI.Conclusively，the fertility of sperm in IVF can be predicted by the kinetics analysis，but that prediction in AI requires further research.

CLC Number:

S823.3+4

HU Ting-xi，LIU Xia，SUN Wei-jun，HAO Hai-sheng，ZHAO Xue-ming，WANG Zong-li，DU Wei-hua，ZHU Hua-bin. Correlation between Sperm Kinetics Parameters and Fertility of Holstein Sex-sorted Sperm in vitro and in vivo[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1727-1732.

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Correlation between Sperm Kinetics Parameters and Fertility of Holstein Sex-sorted Sperm in vitro and in vivo

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