冷诱导RNA结合蛋白参与小鼠H1N1甲型流感病毒感染的应答

doi:doi: 10.11843/j.issn.0366-6964.2016.08.016

Abstract

Abstract:

Cold induced RNA binding protein (CIRP) is a highly conserved multifunctional protein among vertebrates，but its role in viral infection is unclear.The objective of this paper was to explore the response of CIRP to influenza virus infection.The BALB/c mice were intranasal inoculated with H1N1 influenza A virus.Three mice were randomly selected in each group and euthanized at 24，48，72，96，120 h post infection.The heart，liver，spleen and lung of each mouse were collected.Real-time RT-PCR and immunohistochemistry (IMH) were used to detect the transcription of Cirp mRNA and expression of CIRP in each organ.The results of Real-time RT-PCR exhibited that the Cirp mRNA significantly increased in all detected organs.The results of IMH test showed that CIRP protein was highly expressed in cytoplasm of myocardial cells，alveolar epithelial cells，bronchial epithelium cells and spleen lymphocytes.In summary，CIRP is involved in the response to influenza A virus infection，because CIRP can significantly regulate the production of inflammatory factors，the role of CIRP in influenza virus infection should be further studied.

CLC Number:

S852.23

NIE Pei-ting，TANG Cheng，YUE Hua. Cold-inducible RNA Binding Protein of Mice Involved in Response to Influenza A Virus Infection in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1652-1657.

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Cold-inducible RNA Binding Protein of Mice Involved in Response to Influenza A Virus Infection in Mice

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