绵羊肌肉组织MyoG和 PID1 基因的表达及其与肌内脂肪含量的关系

doi:doi: 10.11843/j.issn.0366-6964.2016.10.006

Abstract

Abstract:

The objectives of this study were to investigate the developmental changes of Myogenin (MyoG) and Phosphotyrosine interaction domain containing 1 (PID1) mRNA expression in different parts of muscles in sheep and research its effect on intramuscular fat (IMF) accumulation.Five Aohan fine wool sheep were slaughtered at 2，4，5，6，12 months，respectively，to collect samples from longissimus dorsi and biceps femoris，for determining the IMF content.The mRNA expression of the MyoG and PID1 in 2 parts of muscles was investigated by real-time PCR，and association between their mRNA levels and IMF contents were also analyzed.The results showed that，2-5 month，with growing of the age，the IMF contents of longissimus dorsi and biceps femoris increased.And 5-12 month，the IMF contents remained unchanged.The IMF content of longissimus dorsi were extremely significantly higher than biceps femoris (P<0.01) at the same age.MyoG and PID1 mRNA expression level was different in 2 parts of muscles and had tissues-dependent.In longissimus dorsi， MyoG expression level was the highest (P<0.01) in 5 month old sheep，PID1 expression level was the highest (P<0.05) in 6 month old sheep，there was an increase and then decrease trend in the 2 gene expression.In biceps femoris，the MyoG and PID1 expression level difference was extremely significant(P<0.01) among different month ages，and MyoG expression level presented increased and declined slowly with growing，PID1 expression level presented declined-increased with growing.In the same month，MyoG and PID1 expression level had significant difference in different parts.Correlation analysis showed that MyoG and PID1 expression level was inordinately positively related to IMF content.In conclusion，MyoG expression has positively effect on IMF accumulation in sheep，and PID1 expression is likely to influence on IMF accumulation.

CLC Number:

S826
S813.3

LUAN Zhao-jin，HE Jian-ning，CHENG Ming，LIU Kai-dong，QU Xu-xian，LIU Nan. Association of the MyoG and PID1 Gene Expression with Intramuscular Fat Content in Sheep Muscles [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1986-1994.

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References

［1］赵有璋.羊生产学［M］.北京：中国农业出版社，2002：69-75.
ZHAO Y Z.Production science of sheep and goat［M］.Beijing：China Agriculture Press，2002：69-75.(in Chinese)
［2］URBAN T，MIKOLASOVA R，KUCIEL J，et al.A study of associations of the H-FABP genotypes with fat and meat production of pigs［J］. J Appl Genet，2002，43(4)：505-510.
［3］杜琛，李金泉，陈秀娟.白绒山羊 PPARγ 基因RNA干扰慢病毒载体的构建及对肌内脂肪细胞增殖分化的影响［J］.畜牧兽医学报，2016，47(4)：671-678.
DU C，LI J Q，CHEN X J.Construction of lentiviral RNAi vector of PPARγ gene in cashmere goat and its effect on proliferation and differentiation of intramuscular adipocytes［J］. Acta Veterinaria et Zootechnica Sinica，2016，47(4)：671-678.(in Chinese)
［4］WANG Y H，BYRNE K A，REVERTER A，et al.Transcriptional profiling of skeletal muscle tissue from two breeds of cattle［J］.Mamm Genome，2005，16(3)：201-210.
［5］郝称莉，李齐发，乔永，等.湖羊肌肉组织H-FABP和PPARγ基因表达水平与肌内脂肪含量的相关研究［J］.中国农业科学，2008，41(11)：3776-3783.
HAO C L，LI Q F，QIAO Y，et al.Association of the H-FABP and PPARγ gene expression with intramuscular fat content in Hu sheep muscles［J］.Scientia Agricultura Sinica，2008，41(11)：3776-3783.(in Chinese)
［6］MEUWISSEN T H E，GODDARD M E.The use of marker haplotypes in animal breeding schemes［J］.Genet Sel Evol，1996，28(2)：161-176.
［7］HASTY P，BRADLEY A，MORRIS J H，et al.Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene［J］.Nature，1993，364 (6437)：501-506.
［8］苏艳红，齐鸷，周越，等.Caveolin-3，MyoGenin，MyoD在大鼠力竭运动后骨骼肌损伤过程的时程表达［J］.北京体育大学学报，2012，35(12)：57-61.
SU Y H，QI Z，ZHOU Y，et al.Effect of Caveolin-3，Myogenin and MyoD on the rehabilitation of skeletal muscle injury after rats’exhausting exercise［J］.Journal of Beijing Sport University，2012，35(12)：57-61.(in Chinese)
［9］BERTI F，NOGUEIRA J M，WOHRLE S，et al.Time course and side-by-side analysis of mesodermal，pre-myogenic，myogenic and differentiated cell markers in the chicken model for skeletal muscle formation［J］.J Anat，2015，227(3)：361-382.
［10］杜琛.绒山羊肌内脂肪沉积特征及脂肪细胞分化机理的研究［D］.呼和浩特：内蒙古农业大学，2014.
DU C.Characteristics of intramuscular fat deposition and mechanism of adipocyte differentiation in cashmere goat［D］.Hohhot：Inner Mongolia Agricultural University，2014.(in Chinese)
［11］BOCIAN M，CIESLAK D，GRAJEWSKA S，et al.A relationship between genotypes at MYOG，MYF3 and MYF5 loci and carcass meat and fat deposition traits in pigs［J］.Anim Sci Pap Rep，2002，20(2)：79-92.
［12］ZHU L，LI X W.The genetic effects of MyoG gene［J］.Hereditas (Beijing)，2005，27(5)：710-714.
［13］姜美华，巨婷婷，刘洋，等.成肌细胞成脂过程中PPARγ、C/EBPα和Myogenin基因启动子的甲基化变化［J］.中国农业科学，2013，46(14)：3010-3021.
JIANG M H，JU T T，LIU Y，et al.Dynamics of PPARγ，C/EBPα and Myogenin gene promoter methylation during myoblasts intracellular lipid accumulation［J］.Scientia Agricultura Sinica，2013，46(14)：3010-3021.(in Chinese)
［14］SHI C M，XU G F，YANG L，et al.Overexpression of TFAM protects 3T3-L1 adipocytes from NYGGF4 (PID1) overexpression-induced insulin resistance and mitochondrial dysfunction［J］.Cell Biochem Biophys，2013，66(3)：489-497.
［15］ZHAO Y，ZHANG C，CHEN X，et al.Overexpression of NYGGF4（PID1） induces mitochondrial impairment in 3T3-L1 adipocytes［J］.Mol Cell Biochem，2010，340(1-2)：41-48.
［16］李娟，史春梅，季晨博，等.NYGGF4(PID1)基因过表达对脂肪细胞中脂肪酸合成酶表达的影响［J］.江苏医药，2012，38(19)：2233-2235.
LI J，SHI C M，JI C B，et al.Effect of NYGGF4(PID1) gene over-expression on fatty acid synthase in adipocytes［J］.Jiangsu Medical Journal，2012，38(19)：2233-2235.(in Chinese)
［17］XU H，XU G，WANG D，et al.Molecular cloning and tissue distribution of the phosphotyrosine interaction domain containing 1 (PID1) gene in Tianfu goat［J］.Gene，2013，515(1)：71-77.
［18］殷捷，凌英会，丁建平，等.山羊PID1基因组织表达谱及其与肌内脂肪含量的相关性［J］.江苏农业学报，2014，30(4)：784-789.
YIN J，LING Y H，DING J P，et al.Expression of PID1 and the association with intramuscular fat in goat［J］.Jiangsu Journal of Agricultural Sciences，2014，30(4)：784-789.(in Chinese)
［19］钱源，曾勇庆，杜金芳，等.猪PID1基因CDS区的克隆及其mRNA表达与肌内脂肪沉积关系［J］.遗传，2010，32(11)：68-73.
QIAN Y，ZENG Y Q，DU J F，et al.CDS cloning and relationship between intramuscular fat content and mRNA expression of PID1 gene in pig［J］.Hereditas，2010，32(11)：68-73.(in Chinese)
［20］张晓卉.民猪MyoG基因的克隆、表达与启动子核心区的定位［D］.哈尔滨：东北农业大学，2012.
ZHANG X H.The cloning，expression and the core area promoter positioning of min pig MyoG gene［D］.Harbin：Northeast Agricultural University，2012.(in Chinese)
［21］CHIBA T，YAMAZA H，KOMATSU T，et al.Pituitary growth hormone suppression reduces resistin expression and enhances insulin effectiveness：relationship with calonic restriction［J］.Exp Gerontol，2008，43(6)：595-600.
［22］钱源，曾勇庆，崔景香，等.莱芜猪PID1基因的功能分析及表达谱研究［J］.畜牧兽医学报，2011，42(5)：621-628.
QIAN Y，ZENG Y Q，CUI J X，et al.Functional analysis and tissue specific expression profile of PID1 gene in laiwu pig［J］.Acta Veterinaria et Zootechnica Sinica，2011，42(5)：621-628.(in Chinese)
［23］WU W，GARI W，TONG M，et al.Over-expression of NYGGF4 (PID1) inhibits glucose transport in skeletal myotubes by blocking the IRS1/PI3K/AKT insulin pathway［J］.Mol Genet Metab，2011，102(3)：374-377.
［24］WANG B，ZHANG M，NI Y，et al.Identification and characterization of NYGGF4，a novel gene containing a phosphotyrosine-binding (PTB) domain that stimulates 3T3-L1 preadipocytes proliferation［J］.Gene，2006，379(1)：132-140.
［25］WU D D，IRWIN D M，ZHANG Y P.Molecular evolution of the keratin associated protein gene family in mammals，role in the evolution of mammalian hair［J］.BMC Evol Biol，2008，8：241.
［26］DA COSTA A S，PIRES V M，FONTES C M，et al.Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets［J］.BMC Vet Res，2013，9：118.
［27］GRAUGNARD D E，PIANTONI P，BIONAZ M，et al.Adipogenic and energy metabolism gene networks in longissimus lumborum during rapid post-weaning growth in Angus and Angus×Simmental cattle fed high-starch or low-starch diets［J］.BMC Genomics，2009，10：142.

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Association of the MyoG and PID1 Gene Expression with Intramuscular Fat Content in Sheep Muscles

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