酿酒葡萄皮渣对单栏饲养方式下公绵羊繁殖性能及睾丸抗氧化性的影响

doi:10.11843/j.issn.0366-6964.2016.11.010

Abstract

Abstract:

This experiment was conducted to investigate the effect of wine grape pomace supplementation to base diet on reproductive performance and antioxidant ability of testis in ram. A total of thirty 5-month-old with average weight of (25±1) kg Dorper (♂) × Small Tail Han sheep (♀) F1 male lambs were randomly selected and divided into 5 groups equally. To establish a stress model, lambs in one of groups were raised freely, while the other 4 groups were confined in stall. Lambs raised freely were fed with a base diet without grape pomace, and other lambs were fed with diets supplemented with grape pomace at 0%, 5%, 10%, 20%, respectively. The experiment lasted for 80 d. At the end of experiment, all lambs were slaughtered and sampled. Testis coefficient, epididymal sperm indicators, antioxidant levels of testis were analyzed, and moreover, expression of Cu/ZnSOD, CAT, GPx4, Nrf2 mRNA and protein in testis were detected. The results showed that, compared with free group, MDA and ROS level of testis significantly increased (P<0.01), testis weight of lambs significantly decreased (P<0.05), and the organ coefficient of testis had a trend to be lower (P=0.06) in confined group with 0% wine grape pomace; epididymal sperm density, acrosomal integrity and motility were lower (P<0.05 and P<0.01, respectively), while epididymal sperm deformity higher (P<0.01), in confined group with 0% wine grape pomace than those in free group. These results showed that ram raised in confined condition could cause oxidative stress and affect the reproductive performance. However, compared with confined group with 0% wine grape pomace, epididymal sperm density and motility were higher, while deformity lower, in wine grape pomace-added groups (P<0.05). MDA level of testis significantly decreased (P<0.01), but SOD, CAT and GSH-Px activity significantly increased (P<0.01,P<0.05 and P<0.01) in wine grape pomace-added groups. Furthermore, the mRNA expression of Cu-ZnSOD and protein expression of Cu-ZnSOD, GPx4 and CAT in testis significantly increased (P<0.05) in wine grape pomace-added groups. The results indicate that wine grape pomace can enhance antioxidant capacity in testis and improve the reproductive performance of separated-raising lambs.

CLC Number:

S826.2

JIN Ya-qian, LIU Wen-zhong, ZHAO Jun-xing, REN You-she, ZHANG Chun-xiang, ZHANG Wen-jia, XIANG Bin-wei, MA Xue-hao, ZHANG Jian-xin. Effect of Dietary Wine Grape Pomace on Reproductive Performance, Antioxidant Ability of Testis in Ram[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2228-2239.

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Effect of Dietary Wine Grape Pomace on Reproductive Performance, Antioxidant Ability of Testis in Ram

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