氟对小鼠睾丸谷胱甘肽抗氧化系统的影响

doi:10.11843/j.issn.0366-6964.2017.05.021

Abstract

Abstract:

This study was conducted to investigate the effect of sodium fluoride on the glutathione antioxidant system of mice testis. Twenty-four 3 weeks old ICR male mice were randomly divided into control group and 3 fluoride groups. The four groups were orally administrated with 0, 25, 50, and 100 mg·L^-1 NaF for 60 days. After the exposure treatment, the level of T-AOC, ROS and MDA, the activity of GPx, GST and GR and gene transcription of GPx1, GPx4, GST-mu5 and GR in the testis were detected. The mitochondria, endoplasmic reticulum and Golgi apparatus were observed by electron microscope. Results were as follows: Compared with the control group, the contents of T-AOC was decreased, the contents of ROS and MDA were raised, and the activity of GPx, GST and GR were reduced, and the relative mRNA transcription of GPx1, GPx4, GST-mu5 and GR were down-regulated. It was showed that the antioxidant capacity of testis were decreased and in an obvious oxidative stress state, which related to the decrease of the enzyme activity of glutathione antioxidant system and its related gene transcription.

CLC Number:

S859.8

LI Su-juan, XING Yan-gang, XUE Xing-chen, ZHANG Yu-liang, YU Yu-xiang, ZHAO Yang-fei, NIU Rui-yan, SUN Zi-long. Effect of Fluoride on Glutathione Antioxidant System in Mice Testis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 954-962.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.05.021

https://www.xmsyxb.com/EN/Y2017/V48/I5/954

References

[1] LONG H, JIN Y, LIN M, et al. Fluoride toxicity in the male reproductive system[J]. Fluoride, 2009, 42(4): 260-276.
[2] 关丽萍, 李术. 高氟对动物生殖功能影响的研究概况[J]. 东北农业大学学报, 2008, 39(2): 280-284.
GUAN L P, LI S. Research advances of high fluoride on animal reproduction[J]. Journal of Northeast Agricultural University, 2008, 39(2): 280-284. (in Chinese)
[3] 周健, 杨惠芳. 氟的生殖、遗传毒性和对子代健康影响的研究进展[J]. 现代预防医学, 2008, 35(14): 2640-2642.
ZHOU J, YANG H F. Research progression on the effects of reproduction toxicity and inherent toxicity of fluoride on offspring health[J]. Modern Preventive Medicine, 2008, 35(14): 2640-2642. (in Chinese)
[4] CAO J L, CHEN Y, CHEN J J, et al. Fluoride exposure changed the structure and the expressions of Y chromosome related genes in testes of mice[J]. Chemosphere, 2016, 161: 292-299.
[5] DONG C G, CAO J L, CAO C F, et al. Effects of fluoride and aluminum on expressions of StAR and P450scc of related steroidogenesis in guinea pigs' testis[J]. Chemosphere, 2016, 147: 345-351.
[6] HUO M J, HAN H J, SUN Z L, et al. Role of IL-17 pathways in immune privilege: a RNA deep sequencing analysis of the mice testis exposure to fluoride[J]. Sci Rep, 2016, 6: 32173.
[7] HAN H J, SUN Z L, LUO G Y, et al. Fluoride exposure changed the structure and the expressions of reproductive related genes in the hypothalamus-pituitary-testicular axis of male mice[J]. Chemosphere, 2015, 135: 297-303.
[8] FRENI S C. Exposure to high fluoride concentrations in drinking water is associated with decreased birth rates[J]. J Toxicol Environ Health, 1994, 42(1): 109-121.
[9] LU Z J, WANG S L, SUN Z L, et al. In vivo influence of sodium fluoride on sperm chemotaxis in male mice[J]. Arch Toxicol, 2014, 88(2): 533-539.
[10] ORTIZ-PÉREZ D, RODRÍGUEZ-MARTÍNEZ M, MARTÍNEZ F, et al. Fluoride-induced disruption of reproductive hormones in men[J]. Environ Res, 2003, 93(1): 20-30.
[11] 孙子龙, 牛瑞燕, 王俊东. 氟对雄性小鼠生长发育及性腺中氟含量的影响[J]. 中国畜牧兽医, 2012, 39(3): 227-229.
SUN Z L, NIU R Y, WANG J D. Effect of fluoride on growth and fluoride content of gonads in male mice[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(3): 227-229. (in Chinese)
[12] KUMAR A, SUSHEELA A K. Ultrastructural studies of spermiogenesis in rabbit exposed to chronic fluoride toxicity[J]. Int J Fertil Menopausal Stud, 1994, 39(3): 164-171.
[13] KIM J, KWON W S, RAHMAN M S, et al. Effect of sodium fluoride on male mouse fertility[J]. Andrology, 2015, 3(3): 544-551.
[14] YILMAZ B O, ERKAN M. Effects of vitamin C on sodium fluoride-induced oxidative damage in sertoli cells[J]. Fluoride, 2015, 48(3): 241-251.
[15] SUN Z L, ZHANG W, XUE X C, et al. Fluoride decreased the sperm ATP of mice through inhabiting mitochondrial respiration[J]. Chemosphere, 2016, 144: 1012-1017.
[16] GHOSH D, DAS(SARKAR) S, MAITI R, et al. Testicular toxicity in sodium fluoride treated rats: association with oxidative stress[J]. Reprod Toxicol, 2002, 16(4): 385-390.
[17] DORESWAMY K, SHRILATHA B, RAJESHKUMAR T, et al. Nickel-induced oxidative stress in testis of mice: evidence of DNA damage and genotoxic effects[J]. J Androl, 2004, 25(6): 996-1003.
[18] GAO Q, LIU Y J, GUAN Z Z. Oxidative stress might be a mechanism connected with the decreased α7 nicotinic receptor influenced by high-concentration of fluoride in SH-SY5Y neuroblastoma cells[J]. Toxicol in Vitro, 2008, 22(4): 837-843.
[19] GAO Q, LIU Y J, GUAN Z Z. Decreased learning and memory ability in rats with fluorosis: increased oxidative stress and reduced cholinesterase activity in the brain[J]. Fluoride, 2009, 42(4): 277-285.
[20] SUZUKI M, BANDOSKI C, BARTLETT J D. Fluoride induces oxidative damage and SIRT1/autophagy through ROS-mediated JNK signaling[J]. Free Radic Biol Med, 2015, 89: 369-378.
[21] LUSHCHAK V I. Glutathione homeostasis and functions: potential targets for medical interventions[J]. J Amino Acids, 2012, 2012: 736837.
[22] AKERBOOM T P, BILIZER M, SIES H. The relationship of biliary glutathione disulfide efflux and intracellular glutathione disulfide content in perfused rat liver[J]. J Biol Chem, 1982, 257(8): 4248-4252.
[23] BALLATORI N, KRANCE S M, NOTENBOOM S, et al. Glutathione dysregulation and the etiology and progression of human diseases[J]. Biol Chem, 2009, 390(3): 191-214.
[24] FORMAN H J, ZHANG H Q, RINNA A. Glutathione: overview of its protective roles, measurement, and biosynthesis[J]. Mol Aspects Med, 2009, 30(1-2): 1-12.
[25] MEREDITH M J, REED D J. Status of the mitochondrial pool of glutathione in the isolated hepatocyte[J]. J Biol Chem, 1982, 257(7): 3747-3753.
[26] HWANG C, SINSKEY A J, LODISH H F. Oxidized redox state of glutathione in the endoplasmic reticulum[J]. Science, 1992, 257(5076): 1496-1502.
[27] YUAN L Y, KAPLOWITZ N. Glutathione in liver diseases and hepatotoxicity[J]. Mol Aspects Med, 2009, 30(1-2): 29-41.
[28] FERNÁNDEZ-CHECA J C, KAPLOWITZ N, GARCIA-RUIZ C, et al. GSH transport in mitochondria: defense against TNF-induced oxidative stress and alcohol-induced defect[J]. Am J Physiol, 1997, 273(1): G7-G17.
[29] GARCIA-RUIZ C, FERNÁNDEZ-CHECA J C. Mitochondrial glutathione: hepatocellular survival-death switch[J]. J Gastroenterol Hepatol, 2006, 21(S3): S3-S6.
[30] JIANG Z, HU Z P, ZENG L W, et al. The role of the Golgi apparatus in oxidative stress: is this organelle less significant than mitochondria?[J]. Free Radic Biol Med, 2011, 50(8): 907-917.
[31] LAMBERT A J, BRAND M D. Reactive oxygen species production by mitochondria[M]//STUART J A. Methods in Molecular Biology. Totowa: Humana Press, 2009, 554: 165-181.
[32] PARADIES G, PETROSILLO G, PISTOLESE M, et al. Reactive oxygen species affect mitochondrial electron transport complex I activity through oxidative cardiolipin damage[J]. Gene, 2002, 286(1): 135-141.
[33] OTT M, ROBERTSON J D, GOGVADZE V, et al. Cytochrome c release from mitochondria proceeds by a two-step process[J]. Proc Natl Acad Sci U S A, 2002, 99(3): 1259-1263.
[34] IMANISHI T, TSUJIOKA H, AKASAKA T. Endothelial progenitor cells dysfunction and senescence: contribution to oxidative stress[J]. Curr Cardiol Rev, 2008, 4(4): 275-286.
[35] MAIORINO M, BOSELLO V, URSINI F, et al. Genetic variations of gpx-4 and male infertility in humans[J]. Biol Reprod, 2003, 68(4): 1134-1141.
[36] CASTELLÓN E A. Influence of age, hormones and germ cells on glutathione S-transferase activity in cultured Sertoli cells[J]. Int J Androl, 1999, 22(1): 49-55.
[37] MANN C L, DAVIES M B, BOGGILD M D, et al. Glutathione S-transferase polymorphisms in MS: their relationship to disability[J]. Neurology, 2000, 54(3): 552-557.
[38] EDDY E M, TOSHIMORI K, O'BRIEN D A. Fibrous sheath of mammalian spermatozoa[J]. Microsc Res Tech, 2003, 61(1): 103-115.
[39] 吴承疆, 朱化彬, 王栋, 等. 牛冻、鲜精子差异蛋白的双向电泳和质谱鉴定的初步研究[J]. 中国畜牧兽医, 2010, 37(11): 100-104.
WU C J, ZHU H B, WANG D, et al. Primary study on identify of the differential proteins between bovine frozen and fresh spermatozoa using 2-DE and MS[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(11): 100-104. (in Chinese)
[40] GOPALAKRISHNAN B, SHAHA C. Inhibition of sperm glutathione S-transferase leads to functional impairment due to membrane damage[J]. FEBS Lett, 1998, 422(3): 296-300.
[41] CHEN S S, CHANG L S, CHEN H W, et al. Polymorphisms of glutathione S-transferase M1 and male infertility in Taiwanese patients with varicocele[J]. Hum Reprod, 2002, 17(3): 718-725.
[42] OKUBO K, NAGAHAMA K, KAMOTO T, et al. GSTT1 and GSTM1 polymorphisms are associated with improvement in seminal findings after varicocelectomy[J]. Fertil Steril, 2005, 83(5): 1579-1580.

Effect of Fluoride on Glutathione Antioxidant System in Mice Testis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Guangxing, CHEN Yang, CHEN Kaiting, WU Menglin, ZHANG Di, HUANG Xiaodan. Di (2-ethylhexyl) Phthalate Induces the Apoptosis and Necroptosis of HD11 Cells through the ROS/PTEN/PI3K/AKT Axis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5240-5251.
[2]	YANG Mengran, YANG Chenglin, WU You, WANG Siqi, KONG Xiangyi, NING Can, XIAO Wenguang, FAN Hui, WU Jing, YUAN Zhihang. Study on the Mechanism of Citrinin Induced Intestinal Barrier Dysfunction in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4805-4816.
[3]	GUO Lingjun, ZHU Rui, LUO Yiqian, ZHANG Zhijin, ZHANG Yupeng, ZHANG Dezhi, LI Qianyong. Analysis of Blood Metabolites in Goat Chronic Distiller's Grain Poisoning and Expression Changes of Key Genes in Ethanol Poisoning Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1751-1765.
[4]	HUANG Jingjie, LI Miao, CHEN Yingxian, ZHONG Yalan, ZHANG Tingting, JIANG Tingchaonan, LI Jiancheng. Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Fluorquinolones based on Immunomagnetic Bead Purification [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 766-778.
[5]	DAI Chaohui, LI Hui, ZHAO Weimin, FU Yanfeng, LIAO Chao, LI Bixia, WANG Xuemin, CHENG Jinhua. Research Progress in Toxic Effect of Deoxynivalenol on Pigs and Its Toxicological Mechanism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 24-35.
[6]	ZHANG Kaizhao, HU Hui, XU Zekai, WANG Shiqian, CUI Hongjie, HUANG Xiaohong. Toxicity of Zearalenone on Chicken Embryo Fibroblasts [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1615-1625.
[7]	CAO Qianying, ZHENG Hao, WANG Yaling, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Mechanism of Autophagy Block in PC12 Cells Induced by Zearalenone [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1270-1279.
[8]	LI Yujuan, XIAO Ning, JIA Wendan, LI Xiarong, ZHENG Xiaofeng, SUN Zhiliang. The Effectiveness of Flumazenil-Epinephrine Combination on Treatment for Acute Gelsemium elegans Poisoning [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 938-946.
[9]	WANG Weifu, QIAN Yaguang, LU Ping, HE Shan, DU Ling, LI Yuling, GAO Feng. The Relationship between Swainsonine and Endophytic Fungi in Different Populations of Oxytropis glabra from Inner Mongolia [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 304-314.
[10]	WANG Zilin, TANG Ziheng, LIN Zening, LIU Wenzi, LIU Rong, ZHANG Jiahui, SHEN Xiangguang. Effect of Traditional Chinese Medicine Compound Synephrine on the Detection of β-agonists Using Colloidal Gold Immunochromatographic Assay [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3675-3680.
[11]	ZHAO Wenxing, WU Kexin, GUO Ziyu, ZHANG Yunhao, TANG Lihui, LIU Yiling, MO Chonghui, ZHAO Baoyu, LU Hao. Isolation and Identification of Endophytic Fungi in Thermopsis lanceolata [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2986-2994.
[12]	CHEN Yingxian, MA Yuejiao, LI Jiancheng. A Review of Immunoassay for the Determination of Coccidiostats Residues [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 899-908.
[13]	CAO Yuying, WANG Jie, ZHANG Ximo, WU Qinyao, QIU Jicheng, LI Shuyuan, ZHANG Lu, GONG Xiaohui, BAI Runian, CAO Xingyuan. Residues Depletion and Withdrawal Period Study of Doxycycline Hydrochloride Tablets in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1086-1093.
[14]	WU Fengyang, CUI Jia, YANG Xinyu, CHEN Baojiang. Effect of Zearalenone on Uterine and Ovarian Antioxidant and Inflammatory Indexes and Related Gene Expression in Prepubertal Gilts [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1637-1645.
[15]	WANG Jinming, GAO Yufeng, CHENG Xiaofang, YANG Jiarong, XU Huimiao, ZHAO Yangfei, LI Yanyan. Effects of GSTO1 Gene Silencing on Autophagy and Apoptosis of Fluoride-induced Osteoblasts [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 183-192.